EMAGE mouse gene expression spatial database

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EMAGE:10873

Hspa2 heat shock protein 2 ( MGI:96243)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003311. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003311_01	euxassay_003311_02	euxassay_003311_03	euxassay_003311_04

euxassay_003311_05	euxassay_003311_06	euxassay_003311_07	euxassay_003311_08	euxassay_003311_09

euxassay_003311_10	euxassay_003311_11	euxassay_003311_12	euxassay_003311_13	euxassay_003311_14

euxassay_003311_15	euxassay_003311_16	euxassay_003311_17	euxassay_003311_18	euxassay_003311_19

euxassay_003311_20	euxassay_003311_21	euxassay_003311_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10873_wholemount_strong.wlz

10873_wholemount_moderate.wlz

10873_wholemount_weak.wlz

10873_wholemount_possible.wlz

10873_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10873_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 09 10 11 12 13

submandibular gland primordium

moderate

regional

moderate expression: see section 04 05 06 07 08 14 15 16 17

pancreas

moderate

regional

moderate expression: see section 07 weak expression: see section 08 09 10 15

vibrissa

moderate

regional

moderate expression: see section 03 05 06 17 18 19 20 weak expression: see section 04

medulla oblongata basal plate

weak

regional

weak expression: see section 06 07 13 14

facial vii ganglion

moderate

regional

moderate expression: see section 17 weak expression: see section 02 03 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 16 weak expression: see section 04

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 15 16 17 weak expression: see section 01 02 03 04 18 19

vagus x ganglion

moderate

regional

moderate expression: see section 06

cervico-thoracic ganglion

weak

regional

weak expression: see section 08 14

cervical ganglion

weak

regional

weak expression: see section 07 15

thoracic ganglion

weak

regional

weak expression: see section 11 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 09 10 11 14 15 weak expression: see section 08

lower jaw incisor

moderate

regional

moderate expression: see section 09 10 11 14 15

upper jaw incisor

moderate

regional

moderate expression: see section 10 11 14 15

testis

moderate

regional

moderate expression: see section 03 04 05 06 17 20 weak expression: see section 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6239
Entity Detected:	Hspa2, heat shock protein 2 ( MGI:96243)
Sequence:	sense strand is shown >T6239 GAGGTCTCCGGGAGACGCTAGTCGCGCTCGTGGAGAGTTGTGAGAAGCGACCGTTGGCTCAGGTCCCGGC TTGCGCTTGCCTCTCCTGACGCTTTCGTCCTAACGTTGCTTTGCCTGTTTTTTCAGTCAGGATGTCTGCC CGCGGCCCGGCTATCGGCATCGACCTGGGCACCACTTACTCGTGCGTGGGGGTATTCCAACATGGCAAGG TGGAGATCATCGCCAACGACCAGGGTAACCGCACCACCCCCAGCTACGTGGCCTTCACTGACACCGAGCG TCTCATCGGCGACGCCGCCAAGAACCAAGTGGCCATGAACCCCACAAACACCATCTTCGACGCCAAGCGG CTGATCGGACGGAAGTTCGAGGACGCCACAGTGCAGTCCGATATGAAGCACTGGCCGTTCCGAGTGGTGA GCGAAGGTGGGAAGCCCAAAGTGCAGGTGGAATATAAAGGGGAGATGAAGACCTTCTTCCCTGAGGAGAT TTCTTCCATGGTCCTCACTAAGATGAAGGAGATCGCCGAAGCCTACCTGGGGGGCAAGGTGCAGAGCGCA GTCATCACTGTTCCTGCCTATTTCAACGACTCGCAGCGCCAGGCCACCAAGGATGCGGGCACCATCACCG GCCTCAACGTGTTGCGCATCATCAATGAGCCCACAGCAGCGGCCATCGCCTATGGCCTGGATAAGAAGGG CTGTGCGGGCGGCGAGAAGAACGTGCTCATCTTTGACCTGGGCGGGGGCACCTTCGATGTGTCCATCCTG ACCATCGAGGATGGCATCTTTGAGGTGAAGTCCACGGCCGGCGATACCCACCTGGGTGGCGAAGACTTCG ACAACCGTATGGTCAGCCACCTGGCGGAGGAGTTCAAGCGCAAACACAAGAAGGACATTGGGCCCAACAA GCGCGCTGTGCGCCGGCTGCGCACCGCCTGCGAGCGCGCTAAGCGCACCCTGAGCTCGTCCACGCAGGCC AGCATAGAGATCGACTCGCTCTACGAGGGCGTGGATTTCTACACGTCCATCACCCGCGCCCGCTTCGAGG AGCTCAACGCCGATCTCTTCCGAGGGACCCTGGAGCCGGTGGAAAAGGCGCTGCGCGATGCCAAGCTAGA CAAGGGCCAGATCCAGGAGATAGTGCTGGTGGGCGGCTCAACCCGCATCCCTAAGATCCAGAAGCTCCTG CAAGATTTCTTCAACGGCAAGGAGCTGAACAAGAGCATTAATCCCGACGAGGCGGTGGCCTACGGCGCCG CTGTGCAGGCGGCTATCCTCATCGGCGACAAGTCGGAAAATGTGCAGGATCTGCTGTTACTCGACGTGAC TCCATTGTCGCTCGGCATCGAAACAGCTGGCGGTGTCATGACCCCACTCATCAAGAGAAACACCACGATC CCCACCAAGCAGACGCAGACCTTCACTACCTACTCAGACAACCAGAGCAGCGTGCTGGTGCAAGTGTACG AGGGCGAACGGGCCATGACCAAGGACAATAACCTCTTGGGCAAGTTCGACCTGACTGGGATCCCCCCAGC ACCCCGTGGGGTCCCCCAGATCGAGGTCACCTTTGACATCGATGCCAACGGCATCCTTAACGTCACTGCT GCGGACAAGAGCACCGGTAAAGAAAATAAAATCACCATAACCAACGACAAGGGTCGGCTGAGCAAAGACG ACATTGACCGGATGGTGCAGGAGGCGGAGCGGTACAAATCGGAAGATGAAGCAAATCGCGATCGCGTGGC AGCCAAAAATGCGGTCGAGTCCTATACCTACAACATCAAGCAGACCGTGGAAGACGAGAAACTGAGGGGC AAGATTAGCGAGCAGGACAAAAACAAGATCCTCGACAAGTGTCAGGAGGTGATCAACTGGCTTGACCGAA ACCAGATGGCAGAGAAAGATGAGTACGAACACAAGCAGAAAGAGCTTGAGAGAGTGTGCAACCCCATCAT CAGCAAACTTTACCAAGGCGGTCCAGGCGGCGGCGGCTCCTCTGGAGGGCCCACCATCGAGGAAGTGGAC TAGGCTTGCGCTGGAGTCCGCGTAAACCTCTTTTCCTTTCTTTTCTTTTTTCTTTTTTTTCCTTTTCTCC CTTTTGTTTTGGTTTCTTTTAAATGTCCTTGTGCCAAGTAAGATCTATTATTGGAAGTCTTTAGCCTGGT GCATACATATGAAAGGAAAGGTGCAACAACTTAGTTTAGTTATAAAAGGTTAGTTCTAAAAGTTTGATTT GGGGGAAAAATGAGGTTTCTCTTTAATGCATTTAGAGCGATTGCTGATTTAAGCTCTTTTTGTTGTTGTT AAGCTTACGTATGTACATGGAGATTTGCTTGAAAGGAGAACCCTGATGCTCGCACACCTGACCTGTGGAA GCTTGGTTACACTAAGACACAGGAAAGCTTGAATTGTTTATTTTTATGTACTATGAGATGAAAGTAAGCA CTGCAGTGATATTAAAGACAGGTATATTCTGTAAACACATAAATGCACATTTAAAGTAAAGCTGAAATTG ATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3499661 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3499661 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10871 same embryo
	EMAGE:10869 same embryo
	EMAGE:10874 same embryo
	EMAGE:10870 same embryo
	EMAGE:10872 same embryo
	EurExpress:euxassay_003311 same experiment
	MGI:4825478 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source