EMAGE mouse gene expression spatial database

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EMAGE:10924

Fam199x family with sequence similarity 199, X-linked ( MGI:2384304)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003359. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003359_01	euxassay_003359_02	euxassay_003359_03	euxassay_003359_04

euxassay_003359_05	euxassay_003359_06	euxassay_003359_07	euxassay_003359_08	euxassay_003359_09

euxassay_003359_10	euxassay_003359_11	euxassay_003359_12	euxassay_003359_13	euxassay_003359_14

euxassay_003359_15	euxassay_003359_16	euxassay_003359_17	euxassay_003359_18	euxassay_003359_19

euxassay_003359_20	euxassay_003359_21	euxassay_003359_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10924_wholemount_strong.wlz

10924_wholemount_moderate.wlz

10924_wholemount_weak.wlz

10924_wholemount_possible.wlz

10924_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10924_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

homogeneous

weak expression: see section 10 11 12 13 14

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 18 19 moderate expression: see section 10 16 17

thyroid gland

moderate

regional

moderate expression: see section 14 15

vibrissa

moderate

regional

moderate expression: see section 07 08 weak expression: see section 21 22

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 10 11 15 16

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

weak

regional

weak expression: see section 04 05 06 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 17

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21

vagus x ganglion

weak

regional

weak expression: see section 08 16

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 08 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 14 15 weak expression: see section 16

maturing glomerular tuft

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17 18 19

left lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11

right lung

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6752
Entity Detected:	Fam199x, family with sequence similarity 199, X-linked ( MGI:2384304)
Sequence:	sense strand is shown >T6752 CAGTGACTCGTCGGGCTTGGCGGCGGTGGCGGCGGCTTCTGAGGTTTCAGAGGAGCGGACTGTGTGGCCG GTGGGCTGCTGCGGCCTCGCAGCAACCTTTGTGCAGAGCCCTCCCCTCCTCGGGCGGCTGCGGTGGGCAC ACTCGGGGGCGGAGGCCACCGCGGCCAGGTTAGCGTGGGGGAAGGGGCGAGTGCCAGAGTAGCCCATCTG CAGCCAGTGAGACCAAGCCGGCCCATCTCCCACCATGTCCGACGAGGCGTCGGCCACCACTTCCTATGAG AAGTTTTTAACCCCAGAGGAGCCCTTCCCTTTCCTAGGAGCTCCTCGCGGGGTGGGCACCTGCCCCAGCG AGGAACCTGGCTGCCTGGACATCAGCGACTTCGGCTGCCAGCTCTCCTCCTGCCATCGCACGGATCCGCT CCACCGGTTTCACACCAACAGGTGGAACCTCACTTCGTGTGGTACAAGTGTCGCAAGCTCTGAATGTAGT GAGGAGCTGTTCTCATCCGTTTCTGTTGGAGATCAAGATGACTGCTACTCCCTGTTAGATGATCAAGACT TCACTTCTTTTGACTTGTTCCCCGAGGGGAGTGTCTGCAGTGATGTCTCCTCATCTATCAGCACGTACTG GGATTGGTCAGATAGTGAATTTGAGTGGCAGTTACCAGGCAGTGACATTGCCAGTGGAAGCGATGTGCTT TCTGACGTCATACCCAGTATTCCAAGTTCACCTTGCCTGGTTTCTAAAAAGAAAAACAAACACCGGAATC TAGATGAGCTTGCTTGGAGTGCGATGACAAATGATGAGCAGGTGGAATATATTGAGTATCTGAGCCGTAA AGTCAGTACTGAGATGGGCCTCCGGGAGCAACTTGACATTATTAAAATCATTGATCCTTCTGCTCAGATC TCCCCTACAGACAGTGAGTTCATTATTGAACTTAACTGTCTCACAGATGAAAAACTGAAGCAGGTCAGAA ACTATATCAAGGAGCATAGCCTTCGTCAGCGGCCTACAAGGGAGGCCTGGAAGAGAAGCAACTTCAGCTG TGCAAGCACCAGTGGAGTGAGCGGTGCCAGCGCCAGTGCCAGCAGCAGCAGTGCCAGCATGGTCAGCTCT GCAAGCAGCAGTGGCTCCAGTGTTGGAAACTCTGCTTCAAACTCCAGTGCCAACATGAGCCGAGCACACA GTGACAGCAACCTGTCTGCAAGTGCGGCAGAGCGGATTCGGGATTCAAAAAAGCGATCCAAGCAGCGAAA GCTCCAGCAGAAGGCCTTCCGCAAGAGGCAGCTGAAGGAGCAGAGGCAGGCGCGGAAGGAGAGGCTCAGT GGGCTCTTCCTCAACGAAGAGGTGCTCTCCTTGAAAGTGACTGAGGAAGACCATGAAGCAGATGTTGATG TTTTGATGTAATAGGGTGAATCCGTCAGTGTCCCTTTGAAGCGTTATTCTCTCTTCATTAGTTTACATTC ATCTTGACCAAACTTTTGGTTAGGGTTGTGCTGATGTAATTAATAATTTAGGCCGGTGGTTTTCAGAGAG TAGCCCCAGCACCCTCAGCTTTCAGCATCCCGAAAAGACCTTAGAATTGTGTTTGGGCCCCATTCCAGAG CCACAGGCAGTGTGGGTTTGGAGCAGCACTGTGTTTTAACTACGCCAGACTATTCTCGTGCACATTCTCA TTTAAGGACCACTGGGAAGTGTCTTAACTACTTTAACTTTATGGTGATTAAGTAGGCTTTTCAAAGACTA CTGTATTTACAGTTTGGGAAACTTCAAAGTACTGATATCAAGGAGTTAATTTTGTCAGTATACATTTAGG GTCCGTAATTCAGTTCTCTTCTTAATGGTAAGATTTTTTTTAAGTTTACTTTTTCAAAAGGGCCATAGTA TAATTCTTAGTTCCTAGGGGCAAATCTTTTTTGAGGTGAGGGTGCACATAAGGAATGCATTGCTGTTTAC AATAGTGCCTTCATTAGGTTTAGTTCTGTTTTCACTAATTATTAATGCAAAGGTATAAAAAAGGGCCCTA GTCTTTTCCAATTAGATGTGAGTTTCTTTGTTTTTCTCTACTTTATGTCAAGTGAGAATCATTTCCGCAA GTGATAACTACTGAGAAGTAATGCTACATTAGTGAAATTTTAGACTTAACGTTGTGTTTCAAGTGGGGAT TAAGTTTCAAGTGGAGATTCCAGTTAGGCAATGATACCAATCAAATTTTCCTGTTGTAGCCTTAAAGTTA AGGCTGTCAAAGAATCTCTAAACTAGAACGTTTATCTGTTTCTCTCTATCAAAAAATTTTAATGTAGTAT ATCTCAATTTTATATGTTTGACGTGTGGACAGGTTGATGTTTCAAATTAACTGACAGACCACTTGGAGTT CAACAAACTTTAGAGACTGCTGTGGGTATATAAAGCAGTATGCAGTAGAAAACACCTTTGTTGGTGCCTA GTAGGACTCTTATATCTAAATGTGAAATCATGGAGTTTTTGAAATCATGTTTTCAAAGAATTCATTATCA TGACCTTAGAAATAGCCTGGCTCCTTTTCTTGTATGTGTCTTTGAGCATTGGCATGGGCTAGAGAAGATA CAAACTGAGACTACTGACAATCAATTTGTTTTCTGACTTTCAGGATTTTCACTTTTACTCTTGCATCTAT CTAGAAAGCAACCTATAATCTTTTGAAAAGGCCACGGAGTTCAGAGGTTAATAGAGTTTCAACATCTTTA ACTTTAGTTCTTCAAAGAGATTAACAACTTAAAATTCTTAATTTTTACTTGCTATAAAGGCTTAGATTTT ACTGAATTAAAAACTGAAGTTTTTGTCCTGGTCTGTTGACAGACACCTTTAATCCTAGCGTTCAGGAGGC TGAGGTGAGGATTTGTAAGTTCAAAACAAGCCTAGGCTACATAGTGCTACGAAAATTTTCTATACCTTTA AAATTTTTTATTTTTTTTTTGGTTTCTTTGAAGGCTATAGAATTGAAACTGAGGGTAAAAATTAGTACCT TGCAGCATTGCTGAGGCCTGTGGTTGGGCTTTAACTTTCGAATGCCTACTAAGATCAGAATTTAAAATGC TACGAAGATAAAAGTGTGAATATGTGTGCTTTCAGTTACAGTAGTATTTCTGACTTTCCTCCTACCTCCT TTGGTACTCCATCTTAGTTTAAAGGAACCAAAGAGATCTTGGGGTCAGATCCTAGAATACTTAATAAAGA ATGTACGCAGTTTTCCCTGTAAGTACAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3672969 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3672969 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10920 same embryo
	EMAGE:10919 same embryo
	EMAGE:10923 same embryo
	EMAGE:10922 same embryo
	EMAGE:10921 same embryo
	EurExpress:euxassay_003359 same experiment
	MGI:4824736 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source