EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10929

Mtap methylthioadenosine phosphorylase ( MGI:1914152)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003372. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003372_01	euxassay_003372_02	euxassay_003372_03	euxassay_003372_04

euxassay_003372_05	euxassay_003372_06	euxassay_003372_07	euxassay_003372_08	euxassay_003372_09

euxassay_003372_10	euxassay_003372_11	euxassay_003372_12	euxassay_003372_13	euxassay_003372_14

euxassay_003372_15	euxassay_003372_16	euxassay_003372_17	euxassay_003372_18	euxassay_003372_19

euxassay_003372_20	euxassay_003372_21	euxassay_003372_22	euxassay_003372_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10929_wholemount_strong.wlz

10929_wholemount_moderate.wlz

10929_wholemount_weak.wlz

10929_wholemount_possible.wlz

10929_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10929_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 04 05 06 20 21 22 23

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 11 18 19 20 21

vibrissa

moderate

regional

moderate expression: see section 07 08 23 weak expression: see section 09 10 22

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 11 12 16 17 18

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 15 16 17

metencephalon rest of alar plate marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 weak expression: see section 13 14 15 16 17 18 19 20 21

metencephalon rest of alar plate ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 22 weak expression: see section 13 14 16 17 18 19 20 21

pons mantle layer

moderate

regional

moderate expression: see section 07 08 17

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 weak expression: see section 15 16

ventral grey horn

moderate

regional

moderate expression: see section 10 11 12 15 16 weak expression: see section 13 14

lower jaw incisor

moderate

regional

moderate expression: see section 13 14 17 18 19

upper jaw incisor

moderate

regional

moderate expression: see section 13 14 17 18 19

cranium

moderate

regional

moderate expression: see section 01 02 03 04 05

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 22 23 weak expression: see section 09 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5973
Entity Detected:	Mtap, methylthioadenosine phosphorylase ( MGI:1914152)
Sequence:	sense strand is shown >T5973 ACGCCGGCCCCGCAGTCTCCTCCCGGTCCCACGCTGCGGCCCGCTGTGGACTTCCCTGGTGCAGACATGG CCTCGGGCTCCGCCTGCACGGCGGTGAAGATTGGAATAATTGGTGGAACAGGCTTGGATGATCCCGAAAT TTTAGAAGGAAGAACTGAGAAATATGTGGATACTCCATTCGGCAAGCCATCCGATGCCTTAATTTTGGGG AAGATAAAGAACGTTGATTGTGTACTTCTTGCAAGACACGGCAGACAACACACCATCATGCCTTCAAAAG TCAACTACCAGGCAAACATCTGGGCCTTGAAGGAAGAGGGCTGTACACATGTCATCGTGACCACAGCTTG CGGGTCCTTGAGAGAGGAGATCCAGCCTGGTGACATGGTCATCATTGACCAATTCATTGACAGGACATCC CTAAGGCCTCAGACCTTCTATGATGGGAGTCATTGCAGTGCCAGAGGAGTGTGCCACATCCCCATGGCTG AACCGTTTTGCCCCAAAACAAGAGAGGTCCTCATAGAAACTGCTAAGAAGCTAGGACTTCGGTGCCATTC AAAGGGGACAATAGTCACAATTGAGGGGCCCCGTTTCAGCTCCCGGGCAGAAAGCCTTATATTCCGTACT TGGGGCGCAGATGTTGTCAACATGACCACTGTTCCAGAGGTGGTACTTGCTAAGGAGGCTGGAATTTGCT ATGCAAGCATTGCCATGGCAACCGACTATGATTGTTGGAAGGAGCATGAAGAAGCAGTGTCAGTGGATGG GGTTTTAAAGACCATGAAAGAAAATGCCAATAAAGCCAAAAGTTTACTCCTCACTACTATACCTCAGATA GGGTCGATGGAATGGTCAGAAACGCTCCGTAACCTAAAGAACATGGCCCAGTTTTCCGTTTTACCACCAA GACATTAAAACAGCATGGCTGACACAAGAAGACGTTCTACTTCTAGTCTTTGGGGGATTTTGCTTAAACA AAAATGGGAAAGATCCGCAGCCTTCATGCCTTTGCCTAGAAAGAAAGACACTGTTAGCGACTCAACCAAG ATCCCAACTCCTCCGGGACCCAGAAGGAAAGCAGACAGCCTGCCAAACAGAGCCGGAGAGCACATCTGTC TCACTTTCCCTATTAAACTGACAACAAGGATTACTAGTGAAAACATCCACTACCCTACAGCGCTAAGGAG CCAATACGAGCTCGAGATGGGACTGTGTGATTGTAAACTGGCGCCATGTTTCCGACACTTGGTATTCTAA GAGTTTCTCTTGAAGAAGTTATGAGATATTCCTTCAAAGAATCATGTGTAAAGAAGAATATTACCTGCCA TAGTAAAATAATCAAAACTATCTGAATATCAAAAAAAATTAGCTGTGCCGTGTTTCCGTTATACTTAGGT TATGGAGCAATCAAGTGGAAATCATGTTTCCTCACGAAGAAATGATTAATCTTTTTCTCTACTTTTAAAT ATAATCAAATAATAAAGCTGTAAAAATTAAAATGGACTTAGAGAACTTTGAAACTCCTTTGGTGGTGAAG TGAAGACAACAACAATAATATAACTGCGCTGTGTATGTGAATCAAGATATATAAGCCAAATAACCCCCTT TCCCAAATAAAGAAATAAAGGAAAATGCTGTGTGAGGAGCTTACTAAACCTTTGCTAAGAAAGATCCTGG GTCACCACACAGCTAAGATGATGTAACTGAGAAAGCCAAGGCCGGTGTTTAATGCAAGCCAGTGTGACTC ATCACAGTGTATCGAGAGTTGACACATAATCAGTTGGCAATTTTTTTCTTTTGTTTAAAAGAAGAAAGTA GAAGCCTAACTTTTTATTTTTCAAATTACTGTTGTTACAGGTGCCCTACAGCGCCCGTAAAGGGTCTGTA AAGTGAGTGTGCCTGTGTCTGAAATTGACTTCAAGATAATAAGCTGTTGATTGTAAGCAATAAATGAATT ATTTGCTCCAGTGTTAATGTCGAGCGAGGCCCTGGATAAGTGCAATTGTTACTTCCGCCCATGTTGCAGT GCTCCCATCTCTGGCTGTAAGCAGTCCGTGGAGACTCCGTGAGGCCTGATCAGGGCTTTAGGTTGTTTTT ACTCACTGCAGTGTCAACACTTCTTTCCCCTTAGCCACTGCGGGGTGGGCATCCGTTTTGTTGCCTTTAG AAAATGCTGCTCTCTTCAACATGGAGAATGACTTTCTTCAGTGGCCTTTCTAGCATCATGTGCAGGCTTC TTCCCCTTTTCCTTAATCCTTCATCATGATGAAACATGCATATAATCACTGGGTCTGGCTCAACATGATG CTGTCCGTTCTGTCCATCTTCCTACGGGTATCGTAATTCCATTCTTTTTCTGACAAGATGAAATCTCCTT GTGTATCCTTTCCGCATCCTTATCCATGCATGTGTTGATGGACGTGTGGGCTGCTCCCATAATGTACCTG TTCTGAGTAGTGTTTCAATAAACATCAGTTTGCAGACTTCTCAGAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3485422 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3485422 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10927 same embryo
	EMAGE:10930 same embryo
	EMAGE:10926 same embryo
	EMAGE:10925 same embryo
	EMAGE:10928 same embryo
	EurExpress:euxassay_003372 same experiment
	MGI:4826480 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source