EMAGE mouse gene expression spatial database

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EMAGE:10936

Plekha2 pleckstrin homology domain-containing, family A (phosphoinositide binding specific) member 2 ( MGI:1928144)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003375. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003375_01	euxassay_003375_02	euxassay_003375_03	euxassay_003375_04

euxassay_003375_05	euxassay_003375_06	euxassay_003375_07	euxassay_003375_08	euxassay_003375_09

euxassay_003375_10	euxassay_003375_11	euxassay_003375_12	euxassay_003375_13	euxassay_003375_14

euxassay_003375_15	euxassay_003375_16	euxassay_003375_17	euxassay_003375_18	euxassay_003375_19

euxassay_003375_20	euxassay_003375_21	euxassay_003375_22	euxassay_003375_23	euxassay_003375_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10936_wholemount_strong.wlz

10936_wholemount_moderate.wlz

10936_wholemount_weak.wlz

10936_wholemount_possible.wlz

10936_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10936_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 12 13 14 15 16

vibrissa

weak

regional

weak expression: see section 04 05 06 07 19 20 21 22

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 14

pons ventricular layer

strong

homogeneous

strong expression: see section 13 14

midbrain ventricular layer

strong

regional

strong expression: see section 14

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 weak expression: see section 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 weak expression: see section 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 weak expression: see section 09 18 19 20 21 22 23

vagus x ganglion

weak

regional

weak expression: see section 09 19

ventral grey horn

strong

regional

strong expression: see section 14 15 moderate expression: see section 17 weak expression: see section 13

dorsal root ganglion

moderate

regional

moderate expression: see section 12 13 17 18 19 weak expression: see section 10 11

vomeronasal organ

moderate

regional

moderate expression: see section 05 06 19 20 21 22

meckel's cartilage

strong

regional

strong expression: see section 03 04 moderate expression: see section 05 06 07 08 16 18 19 20 21 22 23 24 weak expression: see section 09 10 11

lower jaw incisor

moderate

regional

moderate expression: see section 12 16

palatal shelf

moderate

regional

moderate expression: see section 08 09 18 19 20 21

upper jaw incisor

moderate

regional

moderate expression: see section 12 16

testis

moderate

regional

moderate expression: see section 06 07 08 weak expression: see section 09 21 22 23

frontal bone primordium

moderate

regional

moderate expression: see section 01 02 03 04

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 21 22 23 24

clavicle

moderate

homogeneous

moderate expression: see section 10 11 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6196
Entity Detected:	Plekha2, pleckstrin homology domain-containing, family A (phosphoinositide binding specific) member 2 ( MGI:1928144)
Sequence:	sense strand is shown >T6196 CAAGCACAGGGGTGGTAGCCCCTCAAGGACTGCCCGGGCAGCGGGTAGGGGAGGAGCGCAGAGAACGTCC CAGGGTGATGTGAACAGAGCCCAGGAATGCCTTATGTGGATCGGCAGAACCGAATCTGTGGGTTTCTGGA TATTGAAGACAATGAGAACAGTGGCAAATTCCTCCGGAGATACTTTATCCTGGATACCCAGGCCAACTGC CTCCTCTGGTACATGGACAATCCCCAGAACCTGGCCGTTGGGGCAGGAGCTGTCGGATCTCTGCAGCTGA CCTACATCTCGAAGGTGAGCATAGCTACCCCAAAGCAGAAACCTAAAACACCATTCTGCTTCGTTATCAA TGCCCTGTCTCAGAGATATTTTCTTCAAGCCAATGACCAGAAAGATCTGAAGGACTGGGTAGAAGCCTTG AACCAAGCCAGCAAGATCACTGTACCCAAAGCTGGGACAGTACCCTTGGCCACAGAAGTTCTCAAAAACT TAACAGCTCCTCCCACCCTAGAGAAGAAGCCGCAGGTGGCCTACAAGACTGAGATCATCGGGGGTGTGGT GGTACAAACGCCTATCAGCCAGAACGGTGGGGATGGGCAGGAAGGGTGCGAGCCAGGGACTCACGCCTTC CTGCGAAGGTCTCAGAGCTACATCCCCACGTCAGGCTGCCGCCCTTCCACTGGGCCTCCCCTCATTAAGA GTGGCTACTGTGTGAAGCAAGGGAATGTGCGGAAGAGTTGGAAACGACGCTTCTTTGCCCTCGATGACTT TACCATCTGCTACTTCAAGTGTGAGCAGGACAGAGAGCCTCTGCGTACCATACCGCTCAAGGATGTTCTC AAGACTCACGAGTGTCTGGTCAAGTCTGGTGATCTCTTAATGAGGGACAACCTGTTTGAAATCATAACCA CCTCCAGGACGTTCTACGTACAGGCGGACAGCCCTGAGGACATGCACAGCTGGATCGAGGGGATTGGAGC AGCTGTCCAGGCTCTGAAGTGCCACCCTAGGGAGCCGTCCTTTTCAAGGTCCATTTCTTTGACTCGACCT GGAAGTTCTACCCTTACAAGCGCGCCTAACTCCATCTTGTCAAGAAGGCGGCCACCAGCAGAAGAGAAAA GAGGTCTCTGTAAGGCCCCTTCGGTGGCCTCCTCCTGGCAACCCTGGACACCTGTCCCCCAGGCTGAGGA AAAGCCGTTGTCGGTGGAGCATGCTCCAGAGGACTCTCTATTCATGCCTAACCCTGGGGAGAGCACAGCT ACAGGGGTGCTGGCAAGTTCTCGAGTCAGGCACAGGTCGGAGCCCCAGCACCCCAAGGAGAAGCCATTTG TATTCAACCTTGATGACGAAAACATACGAACCTCTGATGTGTGATATGCAGTGCCCGTTGCGTGCAGGAG AGCCAGGGGCTGTGACTTATTTTCTCTGCCATGGTAGAGGACAGAGTCTAATGGCACTCACAGTGGAGGG GCTCGTCTAGCTGGCTTTGGTTTTGCTATTATTGACACCATTTATTTAACTGGGGAAAGCCACTAGGTTA GACCCACAGGCATACTCTATTACCTCTTGAATGTCCCCATTTCCCCAGGTTTCCTAGTGAGGGGTTAGTT TTCATACTTCCTGTCTGGAGGAGGCTAGAGTCCAGTTTCACCCATAAGCTTTTCCCTCTTGTATTTCTTT TATAGTCTTGGTTTTGCACAGGATCTCAGGCTAAGAACTGCTTTCATCTACCCCTGTTTATTGACTGAAG TCTGGGGATCAGTCAGACCATTAGCATCCAGACTGACAGGCATCGGGTGAGACTCAGGTTATACAGTTCC GATTGGGATCTATCTGAGAGCGGTCGTAAGAGTCTGGGGAGTGAAAGGTGAATTTTTGGGATATCTCCCC CAGGCAGTTCTTTCAGGATACCTCCCAGGGGAGCAGACAGGACCTCTTGTGAGTCATGAGGGCTAATTAA ACTGCTATAACCACTTGTAGGAATCATGTCGTCCTCTTGATGCTCCATGACCCCAAATGACATAGAACTG AGTGACAGAGCCACCAGTCTATTTTCCGTGTCATGTCCCGAGGATATGCGGTTATCAGGGTCAAATGTTA TGATTGCAGAGACCAGAACAGAGACTTCCCTTGAAACTGAGGTGTGTGTGTGTGTGTGTGTGTGTGTGTG TGTGAAAGAGAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAAACC ACTCATGTTAGGACAATCTCTCTCTCAGAGGCTTACATTAATATAAAGCAGCTACTGAGAAGAGATGGAT GGTTGTTTATTGCAATAAGCATCTTTGATCCATGTTTTCCAGACAGGAGAATGACTCTTTCTTGGCACAG TGAATCTTGAATTCCTGTCTCACAGCTCTTTTATAGCCAGTCGACTTTTGTGTAATGCTAAGTGCTTGTA TACTGTGCAGCCTGCTGGCCCACTTGCTCTTTGCTTTATTGCACTGCCTGTTAAATGAAGTCATGCACAC ACCAGGTGGAACTCTAGCGTCTGATAAGAGCCTATATACTCACTAGTGAAGAGACTTAAATGTGATTTTT CCCCCCCAGTACTTACATTTTTCATGGAATAGAAACTGGGAATAAAGAAATTGAATCAGAGGATGAATTG TTGGCTAGGAATTTCCTAGGTTCTAGAAGAGTTTAGAAGATTAGATTTCCACCCAAAATTTAGGGTATGG CTTTTCCACTGGGCAAGATACTATTTCCAGTGCCTTTGGGGTCATTTCTATCAGCCTTCAATGAACTGTG CTACCAGAAAATTATGGCCATTTCCTACAGGGAGAGAGGTCATGGCTCATTCCTATCTGTGTAACTTTTA TCTGCTAGGTAAATTAACCCTGTTTTTAATTAGAAATTTGTAGCTCTAAGAGACTAGGAGGCATTTTTTA TTTAAGCTAGTTAGCACTCTGTGTCCATAGTTAATGCAAAAGCAAAAACAGGCATAAAGCTCTCCGGGTG AGAGGAAGCATGCTCTTTCAAATGTGACTGTGACCCTGAATCTGGTGCCACACTGAAGACTTCTGGGCTC CGTGTGTGGGAGCTGTCCCCCGACTTTGTCTGGCCTTGTGATCCTGATGAAAAGAAAACCAAACAAAAAT CAGTGGGCTTCTTTCTCTTTCTGTTCATGATCTTGTGTGTCTCCCATCCCCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498236 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498236 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10934 same embryo
	EMAGE:10933 same embryo
	EMAGE:10938 same embryo
	EMAGE:10935 same embryo
	EMAGE:10937 same embryo
	EurExpress:euxassay_003375 same experiment
	MGI:4827263 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source