EMAGE mouse gene expression spatial database

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EMAGE:11166

Cdk5rap1 CDK5 regulatory subunit associated protein 1 ( MGI:1914221)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003468. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003468_01	euxassay_003468_02	euxassay_003468_03	euxassay_003468_04

euxassay_003468_05	euxassay_003468_06	euxassay_003468_07	euxassay_003468_08	euxassay_003468_09

euxassay_003468_10	euxassay_003468_11	euxassay_003468_12	euxassay_003468_13	euxassay_003468_14

euxassay_003468_15	euxassay_003468_16	euxassay_003468_17	euxassay_003468_18	euxassay_003468_19

euxassay_003468_20	euxassay_003468_21	euxassay_003468_22	euxassay_003468_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11166_wholemount_strong.wlz

11166_wholemount_moderate.wlz

11166_wholemount_weak.wlz

11166_wholemount_possible.wlz

11166_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11166_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 16 17

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19 20 21 not examined expression: see section 12

pancreas

moderate

regional

moderate expression: see section 11 12

adenohypophysis

moderate

regional

moderate expression: see section 11 12 13 14 15 weak expression: see section 16

vibrissa

moderate

regional

moderate expression: see section 07 08 09 22

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22

naris

moderate

regional

moderate expression: see section 13 14

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 16 17 18 19 20

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 14 17 18

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 17

upper jaw incisor

moderate

regional

moderate expression: see section 13

liver

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

metanephros

moderate

regional

moderate expression: see section 09 10 11 12 19 20 21 22

testis

moderate

regional

moderate expression: see section 08 09 10 11 22 23

lung

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6725
Entity Detected:	Cdk5rap1, CDK5 regulatory subunit associated protein 1 ( MGI:1914221)
Sequence:	sense strand is shown >T6725 GGAGATTTGGGGCTGCAGGTGCCATGCATCCTTTACGGTGTGTCCTCCAAGTACAGAGGTTGTCAGCACC ATTCACCTCCATGTGCTGGGTGTTGCTTAGGACCTGCAGAGCACAAAGCAGTGTGTCCAGCACTCCTTGT CCCAGTCCAGAGGCGAAGAGCTCAGAAGCTCAGAAGGACTTCAGCTCCAGGCTAGCCACTGGACCGACTT TTCAGCATTTTTTAAGAAGTGCTTCAGTTCCTCAAGAGAAACCATCTTCTCCAGAGGTGGAGGACCCACC TCCCTATCTCTCGGGGGATGAACTTCTAGGAAGGCAGAGAAAAGTCTACCTCGAGACCTATGGCTGTCAG ATGAACGTGAACGACACAGAGATAGCCTGGTCCATCTTACAGAAGAGTGGCTACCTTCGGACCAGCAACC TCCAAGAGGCTGATGTGATCCTTCTTGTCACGTGTTCTATCAGGGAGAAGGCCGAGCAGACCATCTGGAA CCGCTTACATCAGCTCAAAGTCCTGAAGACAAAGCGGCCACGCTCTCGAGTACCTCTGAGGATTGGGATT CTAGGCTGCATGGCTGAGAGGCTGAAGGGAGAGATCCTCAACAGGGAGAAGATGGTAGATCTCTTGGCTG GTCCAGACGCCTACCGAGACCTTCCCCGGCTGCTGGCTGTGGTGGAGTCAGGTCAGCAAGCAGCAAATGT GCTTCTCTCTCTGGATGAGACCTACGCAGATATCATGCCAGTCCAGACGAGCCCCAGTGCCACTTCTGCC TTTGTATCCATTATGCGGGGCTGCGACAACATGTGCAGTTACTGTATCGTGCCGTTCACGCGTGGCCGGG AGAGGAGCCGGCCAGTTGCCTCCATTCTCGATGAAGTGAGGAAGCTGTCCGAGCAGGGCCTAAAAGAAGT GACACTTCTTGGTCAGAATGTTAATAGTTTTCGGGACAATTCGGAAGTCCAGTTCAATAATGCAGGGTCT GCTAACCTCAGCCGTGGCTTTACCACCAACTATAAACCCAAACAAGGAGGGCTTCGTTTTTCTCACCTTC TGGATCAGGTTTCAAGAATCGATCCTGAAATGAGGATCCGATTCACCTCTCCTCACCCCAAGGATTTTCC TGATGAGGTTCTTCAGCTGATTCGTGAGAGACACAACATCTGTAAGCAGATCCACCTGCCAGCCCAGAGT GGAAGCAGCCGTGTACTGGATGCCATGCGGAGAGGATATTCAAGAGAAGCATATGTGGCATTAGTTCATC ATGTCAGAGAGACTATCCCAGGTGTGAGCCTTAGCAGCGACTTCATCACTGGCTTTTGTGGAGAGACAGA GGATGATCACCGGCAGACAGTGTCTTTACTTAGAGAAGTGCAGTATAATACCGGTTTTCTCTTTGCATAC AGCATGAGGCAAAAGACACGAGCATATCACAGGCTGAAGGATGATGTTCCAGAAGAAGTAAAATTAAGGC GTTTGGAGGAACTCATTACTGTCTTCAGAGAAGAAGCTTCAAAAGCCAATAAGACCTCAGTGGGTTGTAG CCAGCTGGTACTGGTTGAAGGGTTCAGCAAACGTTCTACCACAGACCTTTGTGGCCGGAATGATGCAAAC CTTAAGGTGATTTTCCCTGACGCAGAGGTGGAGGATATCACTAACCCTGGGCTCAAGGTCAGAGCTCAAC CTGGGGACTATGTGCTGGTGAAGATCACCTCTGCCAGCTCTCAGACCCTGAAAGGACACATTCTCTGCAG GACCACTATGAAAGACTCATTGACGTATTGCACGACCTGAGCTGCTGGATTAGGAGTGGACTTGGGCAGG TTTTTCTCATGCTTTGGAGACATGTAATAGTCTTGAAGCACCTTTGCACCAAATGGAAGAGTACATTTCT TTGTTGTCGATAGAACAACTGTTACAATAATAAATTTCATAAATTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3666341 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3666341 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11169 same embryo
	EMAGE:11164 same embryo
	EMAGE:11168 same embryo
	EMAGE:11165 same embryo
	EMAGE:11167 same embryo
	EurExpress:euxassay_003468 same experiment
	MGI:4823781 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source