EMAGE mouse gene expression spatial database

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EMAGE:11167

Xrcc6 X-ray repair complementing defective repair in Chinese hamster cells 6 ( MGI:95606)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003500. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003500_01	euxassay_003500_02	euxassay_003500_03	euxassay_003500_04

euxassay_003500_05	euxassay_003500_06	euxassay_003500_07	euxassay_003500_08	euxassay_003500_09

euxassay_003500_10	euxassay_003500_11	euxassay_003500_12	euxassay_003500_13	euxassay_003500_14

euxassay_003500_15	euxassay_003500_16	euxassay_003500_17	euxassay_003500_18	euxassay_003500_19

euxassay_003500_20	euxassay_003500_21	euxassay_003500_22	euxassay_003500_23	euxassay_003500_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11167_wholemount_strong.wlz

11167_wholemount_moderate.wlz

11167_wholemount_weak.wlz

11167_wholemount_possible.wlz

11167_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11167_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 04 05 06 07 08 20 21 22 23

thymus primordium

strong

regional

strong expression: see section 11 12 13 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 17 18 19 20

pancreas

moderate

regional

moderate expression: see section 11 12 weak expression: see section 13 16 18

vibrissa

moderate

regional

moderate expression: see section 09 21 22 23 weak expression: see section 07

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 17 18

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 weak expression: see section 09 10 11

liver right lobe

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22 23 24

testis

moderate

regional

moderate expression: see section 08 09 10 weak expression: see section 07 21 22 23

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6729
Entity Detected:	Xrcc6, X-ray repair complementing defective repair in Chinese hamster cells 6 ( MGI:95606)
Sequence:	sense strand is shown >T6729 CGTGGACGTGGTGCCCACCTTCTGCTGTCCCAGTGGCTTGCCCGCCTGCGGTTAAGTGAGCAAACCAACA TGTCAGAGTGGGAGTCCTACTACAAAACTGAGGGCGAGGAAGAGGAAGAGGAGGAGGAGAGCCCCGACAC AGGTGGAGAATATAAATATTCAGGAAGAGATAGCTTGATTTTTCTGGTTGATGCCTCCAGGGCTATGTTC GAATCTCAGGGTGAAGATGAACTCACACCTTTTGATATGAGCATCCAGTGTATCCAGAGTGTGTACACCA GTAAGATCATAAGCAGCGATCGGGATCTCCTGGCAGTGGTGTTCTATGGAACCGAGAAAGATAAAAATTC AGTGAATTTCAAAAATATTTATGTCTTACAAGATTTGGACAACCCAGGCGCTAAGCGAGTGCTAGAGCTC GACCAGTTTAAGGGACAACAGGGGAAGAAGCACTTCCGAGACACGGTTGGCCATGGGTCTGACTACTCTT TGAGTGAAGTGCTCTGGGTCTGTGCCAACCTCTTCAGCGACGTCCAGCTCAAGATGAGTCACAAGAGGAT CATGCTGTTCACCAATGAAGACGACCCCCATGGCCGTGACAGTGCTAAAGCCAGCCGGGCCAGGACCAAA GCCAGCGACCTCCGGGACACTGGGATCTTCCTTGACTTGATGCATCTGAAGAAGCCAGGAGGCTTTGATG TATCCGTGTTCTACAGGGACATCATCACCACCGCTGAGGACGAGGACCTTGGGGTTCACTTCGAGGAGTC AAGCAAGCTGGAAGACCTGCTAAGGAAGGTTCGAGCCAAGGAGACCAAAAAGCGAGTTCTGTCCAGGTTA AAGTTTAAGCTCGGTGAAGACGTAGTACTCATGGTGGGCATTTATAACTTGGTCCAGAAAGCTAACAAGC CTTTTCCAGTGAGACTCTATCGGGAAACAAATGAACCAGTGAAAACCAAGACAAGGACTTTTAATGTAAA CACCGGCAGTCTACTCCTGCCTAGTGACACCAAGCGGTCTCTGACTTACGGGACACGTCAGATTGTGCTG GAGAAAGAGGAGACAGAGGAGCTGAAGCGGTTTGATGAGCCAGGTTTGATCCTCATGGGCTTTAAGCCCA CGGTGATGCTGAAGAAGCAGCACTACCTGAGGCCCTCTCTGTTCGTGTACCCAGAGGAGTCCCTGGTCAG TGGGAGCTCAACCTTGTTCAGCGCTCTGCTCACCAAGTGTGTGGAGAAGAAGGTCATAGCAGTGTGTAGA TACACACCCCGGAAGAACGTCTCCCCGTATTTTGTGGCTTTGGTGCCCCAGGAAGAGGAGCTGGATGATC AGAACATTCAGGTGACTCCAGGAGGCTTCCAGCTTGTCTTCCTCCCTTATGCCGATGACAAGCGGAAGGT GCCCTTTACTGAGAAGGTGACGGCCAACCAGGAGCAGATAGACAAGATGAAGGCCATTGTTCAAAAGCTC CGCTTCACATACAGGAGCGACAGTTTTGAGAATCCAGTCCTGCAGCAGCACTTCCGCAACCTGGAGGCCC TAGCTTTGGACATGATGGAGTCGGAGCAAGTGGTAGATCTGACACTACCCAAGGTTGAAGCCATAAAGAA AAGACTGGGTTCCCTGGCAGATGAGTTTAAAGAACTTGTCTATCCTCCAGGTTATAATCCCGAGGGAAAA GTTGCCAAGAGAAAACAAGATGATGAAGGTTCTACGAGTAAAAAGCCCAAGGTAGAGTTATCAGAAGAAG AGCTGAAGGCCCATTTTCGTAAGGGCACACTGGGTAAGCTCACTGTACCTACACTGAAGGACATATGCAA GGCTCATGGGCTTAAGAGTGGGCCGAAGAAGCAGGAACTGCTAGATGCTCTTATCAGACACTTGGAGAAG AACTGACTAAAGCTCAGAAGCCCAGCCACTTTCTACACTGCAGCCAGGCCACCTGCTTGTGTTCTTACCA AGCTAGGTGGTAGCTACAAAGAGCTACTTAAGTGCAAAATCATGGAGATTACAGTCTCCCACCTCACTGT GCCTTACTCTGTGAATAAAGAGCTCTAACTTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3669715 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3669715 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11169 same embryo
	EMAGE:11166 same embryo
	EMAGE:11164 same embryo
	EMAGE:11168 same embryo
	EMAGE:11165 same embryo
	EurExpress:euxassay_003500 same experiment
	MGI:4829247 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source