EMAGE mouse gene expression spatial database

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EMAGE:11220

Gdap2 ganglioside-induced differentiation-associated-protein 2 ( MGI:1338001)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003406. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003406_01	euxassay_003406_02	euxassay_003406_03	euxassay_003406_04

euxassay_003406_05	euxassay_003406_06	euxassay_003406_07	euxassay_003406_08	euxassay_003406_09

euxassay_003406_10	euxassay_003406_11	euxassay_003406_12	euxassay_003406_13	euxassay_003406_14

euxassay_003406_15	euxassay_003406_16	euxassay_003406_17	euxassay_003406_18	euxassay_003406_19

euxassay_003406_20	euxassay_003406_21	euxassay_003406_22	euxassay_003406_23	euxassay_003406_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11220_wholemount_strong.wlz

11220_wholemount_moderate.wlz

11220_wholemount_weak.wlz

11220_wholemount_possible.wlz

11220_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11220_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 19 20 21 22 23 24

vibrissa

weak

regional

weak expression: see section 04 05 06 18 20

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 09 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 18 19

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 11 17

cervical ganglion

moderate

regional

moderate expression: see section 10 17

thoracic ganglion

moderate

regional

moderate expression: see section 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 17 18 19

not examined

homogeneous

not examined expression: see section 01 02 22 23 24

neural retina

moderate

regional

moderate expression: see section 01 02 22 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 14 15 16 17 18

vomeronasal organ

moderate

regional

moderate expression: see section 10 11 weak expression: see section 14

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10

liver right lobe

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20 21 22 23 24

orbito-sphenoid

weak

regional

weak expression: see section 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6816
Entity Detected:	Gdap2, ganglioside-induced differentiation-associated-protein 2 ( MGI:1338001)
Sequence:	sense strand is shown >T6816 CGCTTCAGCCTCCGCCTCTGGCAAGCAAAGTGTACGGATGTCATCCGCCTGAATTCTGCCACGGATCTGA GTGAGCTGCGGGCCGCCGCCCGGATGGGCCCGCCCGAGCGCTCTCAAATCTCCAGGGATTGTTGAATTGC CAAAAAGTTTGGTTCACGAGCTGAAGCTCCCATTCCATGGATCCCTTGGGTGCACCTTCCCAGTTTGTGG ATGTGGATACCCTGCTAAGCTGGGGCGACTCGTACGAAGATGAAGTAGACTGCGCTGACAGTACTGCCGA GGCCTTCCAGGAAGACGCCAGCAGATCACCTTTTGTTTATAGTCGGGACGTCAATGGAAAAGTGGTTCTT TGGAAAGGAGATGTGGCATTACTGAACTGCACAGCCATTGTGAATACCAGCAATGAAAGCCTCACAGATA AGAACCCTGTGTCAGAGAGCATCTTCATGCTTGCGGGGCCAGATTTGAAGGAGGACCTTCAGAAACTCAA AGGTTGCCGAACTGGTGAAGCAAAATTGACGAAAGGCTTTAACCTAGCTGCTCGGTTCATCATTCACACA GTGGGACCTAAGTACAAGAGCCGCTACCGCACTGCAGCGGAGAGTTCCCTGTACAGCTGTTACAGAAACG TGCTTCAGCTGGCAAAAGAACAGTCCATGTCTTCTGTTGGCTTCTGTGTCATCAATTCTGCCAAGCGAGG TTATCCACTAGAAGATGCAACACACATCGCTCTTCGTACTGTGAGGCGGTTCCTAGAGATCCATGGGGAA AACATTGAAAAAGTAGTATTTGCTGTCTCAGAACTCGAAGAGGCCACTTACCAAAAGCTGCTACCTCTGT ACTTCCCAAGGTCATTAAAGGAGGAGATCCGGTCATTGCCGTACCTACCTGCAGACATTGGAAATGCAGA AGGGGAGCCCGTGGTGCCCGAGCGGCAAATTAGAATCAGTGAGAAACCCGGTGCCTCGGAGGACAATGAA GAGGAAGATGAGGATGAAGGCTTGGGAGTGGATCTGTCTTTTATTGGCTCTCATGCTTTTGCTCGGATGG AAGGAGATATTGACAAGCAAAGAAAGTTGATCCTCCAGGGACAGTTGTCTGAAGCCGCCCTGCAGAAGCA GCATCAAAGAAATTATAATCGCTGGTTATGCCAAGCAAGATCTGAAGACCTGTCTGATATTGCTTCTCTG AAAGCTTTATACCAAACAGGGGTAGATAACTGTGGCCGCACAGTGATGGTGGTAGTTGGAAGAAACATTC CTGTGACACTGATAGATATGGACAAGGCCCTCTTGTATTTTATCCATGTAATGGATCACATTGCTGTGAA GGAGTATGTGCTGGTGTATTTCCACACACTGACCAGCGACTACAATCACCTGGACTCCGACTTCCTGAAG AAACTCTACGATGTGGTTGACATCAAATACAAGAGGAATTTGAAGGCTGTTTATTTCGTTCATCCAACAT TCCGTTCAAAGGTCTCAACATGGTTTTTCACCACCTTTTCTGTCTCAGGACTGAAGGACAAGATCCACCA CGTAGACAGCCTGCAGCAGCTCTTCTCTGCCATATCACCAGAGCAGATAGACTTTCCTCCTTTTGTCCTT GAATATGACGCGAGGGAAAATGGGCCTTATTTTGCATCTTATCCTCCATCACCAGATTTGTGACCTGCGC CTTCCCAGTGCTTCTCGGTCACAGGATTCCATTTAACCACAACTCGCCACCTATCGGAGTGATGTTCATT TTTGCTGTACAGATCCAGAGAGCTTTTGTCCCCACCTCTCTGGTATTTTTTTATTGACTATATATTTTCT GGCACATAAACAATCTAAAACTGGTAGGCCATTCTGAACTGCATACATTTTAAATTTGTATATTTGTATC AAATGGAAATGCTCATTTTTAGAGCATTAATAGAAACTGGGGATCAACTTTTGAGTGTCTCCAGTTTCCT AAAGGACACTGGACTCTGTCACACAGGCCACCAACGTTGCTCACATCATCTCTTACAGTGCACGCTTCCG GACTCTGTTACATAGGCCGCCACCATTGTTCATAGCATCTCTTAAACAGTGCACGCTCTCTCTGTGTATT TAAGTGTTTCTCAAAATATATAGAACCAATGTATGCTAAACGGATGCATTGTTCCCTCAGATCCTGGCTT TAATACCATTTACATTTTATACAGACGATCTGATTATAATATAGAATTGCCCATGACCAAGAATATTGAA ATGTGTATGATCAAAACTGTAAAAACCTTCAACAACATAGAATTGGCAGATTGTTGGCCCAAGCTACAGC TGAGCTGTGCGTGCTTCTTGGGTTGTCTACTTTGTTCCTATTGACACTGCTCTGATCTCAGTGTGGAGAG CATGAGACTGTACCTTCAACTGTCTTCTTTAATCATGTGACTCGTGACTCGTCTCTTTACCAAAAGAATG TTTGTGCTCCCAGCACTCCTAAGTAAAGACCATTTCCCAGGACTCCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3710712 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3710712 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11223 same embryo
	EMAGE:11219 same embryo
	EMAGE:11222 same embryo
	EMAGE:11221 same embryo
	EurExpress:euxassay_003406 same experiment
	MGI:4825030 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source