EMAGE mouse gene expression spatial database

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EMAGE:11231

Acot1 acyl-CoA thioesterase 1 ( MGI:1349396)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003190. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003190_01	euxassay_003190_02	euxassay_003190_03	euxassay_003190_04

euxassay_003190_05	euxassay_003190_06	euxassay_003190_07	euxassay_003190_08	euxassay_003190_09

euxassay_003190_10	euxassay_003190_11	euxassay_003190_12	euxassay_003190_13	euxassay_003190_14

euxassay_003190_15	euxassay_003190_16	euxassay_003190_17	euxassay_003190_18	euxassay_003190_19

euxassay_003190_20	euxassay_003190_21	euxassay_003190_22	euxassay_003190_23	euxassay_003190_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11231_wholemount_strong.wlz

11231_wholemount_moderate.wlz

11231_wholemount_weak.wlz

11231_wholemount_possible.wlz

11231_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11231_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 08 09 17 moderate expression: see section 07 10 11 18 19 20 21

foregut-midgut junction

weak

regional

weak expression: see section 16 18 19

epidermis

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 17 18 21 22 23 24

vibrissa

moderate

regional

moderate expression: see section 05 24 weak expression: see section 06 07 08 23

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15 16

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 12 13 14 17 18 19

telencephalon ventricular layer

strong

regional

strong expression: see section 04 05 08 09 21 moderate expression: see section 01 02 03 06 07 10 11 12 13 14 15 16 17 18 19 20 22 23 24

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16

metencephalon rest of alar plate ventricular layer

strong

regional

strong expression: see section 07 08 09 16 17 18 19 20 21 moderate expression: see section 04 05 06

pons ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 moderate expression: see section 06

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

spinal cord ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15

naris

moderate

regional

moderate expression: see section 17 weak expression: see section 18

nasal septum

moderate

regional

moderate expression: see section 17 weak expression: see section 18

hindgut

weak

regional

weak expression: see section 14 15 16 17 18 19

rectum

weak

regional

weak expression: see section 16

midgut

moderate

regional

moderate expression: see section 12 13 21 weak expression: see section 14 15 16 17 18 19 20

left lung

strong

regional

strong expression: see section 04 05 08 09 10 11 12 moderate expression: see section 06 07 13

right lung

strong

regional

strong expression: see section 16 17 moderate expression: see section 15 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4895
Entity Detected:	Acot1, acyl-CoA thioesterase 1 ( MGI:1349396)
Sequence:	sense strand is shown >T4895 CCACGCGTCCGGCTCGAGATGGAGGCGACGCTGAACCTTGAGCCCTCGGGCCGCAGCTGCTGGGACGAAC CGCTGAGCATCGCCGTGCGCGGCCTGGCCCCGGAGCAGCCCGTCACGCTGCGCTCTGTCCTGCGCGACGA GAAGGGCGCGCTCTTCCGAGCCCACGCGCGCTACCGCGCGGACTCCCACGGCGAGCTGGACCTGGCGCGC ACGCCCGCGCTGGGCGGCAGCTTCTCGGGGCTCGAGCCCATGGGGCTGCTCTGGGCCATGGAGCCCGATC GGCCTTTTTGGCGATTGGTCAAGCGCGACGTGCAGACGCCCTTCGTGGTGGAGCTGGAGGTGCTGGACGG ACACGAGCCCGACGGCGGGCAGCGGCTTGCACATGCGGTGCACGAGCGTCACTTCTTGGCTCCGGGGGTG CGGCGCGTGCCCGTACGAGAGGGCCGGGTGCGCGCCACGCTCTTCCTGCCCCCAGAACCTGGACCCTTTC CTGGGATCATAGACCTTTTTGGAGTTGGAGGTGGCCTTCTGGAGTATCGGGCGAGCCTGCTGGCTGGGAA GGGCTTTGCCGTCATGGCTCTGGCTTATTACAACTACGATGACCTCCCCAAGAACATGGAAACCATGCAC ATGGAGTACTTTGAAGAAGCCGTGAACTACCTGCGCAGCCACCCCGAGGTAAAAGGACCTGGAATTGGGC TGCTTGGGATTTCCAAAGGGGGTGAACTTGGCCTTGCTATGGCCTCCTTCCTGAAGGGCATCACAGCTGC TGTGGTCATCAATGGCTCCGTGGCTGCTGTTGGGAACACCATCTCCTACAAGGATGAGACTATACCCCCT GTGACTATCCTGAGAAATCAGGTCAAAATGACCAAGGATGGCCTCAAGGATGTTGTAGATGCTTTGCAAA GCCCTCTGGTAGACAAGAAGAGCTTCATTCCCGTGGAAAGGTCTGACACGACCTTCCTGTTCCTCGTAGG TCAGGACGACCACAACTGGAAGAGCGAGTTCTATGCTGATGAGATCTCCAAACGCCTGCAGGCGCACGGG AAGGAGAAGCCCCAGATCATCTGCTACCCAGCAGCAGGGCATTACATCGAGCCCCCTTACTTCCCGCTGT GCAGCGCTGGCATGCACCTCCTGGTGGGTGCCAACATCACCTTTGGAGGGGAGCCCAAGCCTCATGCCAT GGCCCAGTTGGATGCATGGCAGCAGCTCCAGACTTTCTTCCACAAACAGTTGGGTAGTGAGTGTTTGCAT GTGTCTCCTAAAATATAACCTATTATATGATGGTTTGGAGGTTGGGGAAAGGTACAAATACATTGTAAGA ATATCATTTCAGATGACTTCATCTGAACATAAAACTGCTTTAGATTTACATTTAAAAAAATTGATATAAT TATCAAAATAGTGCTGATTCAAGGGCTGGGAATGGAGTTTCATTGTCAAGTCCTTGACTGGCGCATGCAG GATCCTGGGTTCAATCCAGCTGCGAGAAATAAAGCACGTTCTATGGAAACTGGAGCTATCCAAGAAAAGT GCCAGGAATAAAAAGGAACCATGATTAAACACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5100478 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5100478 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11233 same embryo
	EMAGE:11232 same embryo
	EMAGE:11230 same embryo
	EMAGE:11229 same embryo
	EMAGE:11228 same embryo
	EurExpress:euxassay_003190 same experiment
	MGI:4822923 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source