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EMAGE:11310

Eny2 enhancer of yellow 2 homolog (Drosophila) ( MGI:1919286)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11310 EMAGE:11310 EMAGE:11310 EMAGE:11310 EMAGE:11310
"Pseudo-wholemount" of euxassay_006635. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006635_01 euxassay_006635_02 euxassay_006635_03 euxassay_006635_04
EMAGE:11310 EMAGE:11310 EMAGE:11310 EMAGE:11310 EMAGE:11310
euxassay_006635_05 euxassay_006635_06 euxassay_006635_07 euxassay_006635_08 euxassay_006635_09
EMAGE:11310 EMAGE:11310 EMAGE:11310 EMAGE:11310 EMAGE:11310
euxassay_006635_10 euxassay_006635_11 euxassay_006635_12 euxassay_006635_13 euxassay_006635_14
EMAGE:11310 EMAGE:11310 EMAGE:11310 EMAGE:11310 EMAGE:11310
euxassay_006635_15 euxassay_006635_16 euxassay_006635_17 euxassay_006635_18 euxassay_006635_19
EMAGE:11310
euxassay_006635_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11310Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11310_wholemount_strong.wlz
11310_wholemount_moderate.wlz
11310_wholemount_weak.wlz
11310_wholemount_possible.wlz
11310_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11310_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
olfactory cortex ventricular layer
moderate moderate
regionalmoderate expression: see section 10 11 14 15
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7076
Entity Detected:Eny2, enhancer of yellow 2 homolog (Drosophila) ( MGI:1919286)
Sequence:sense strand is shown

>T7076
GTAGCTTTCTGTGTGCTTAGGTGCCCGAGCTACTGAGGGTCTAAGTCCGGGCAGCCGAAGAGTGTGGTAG
GTAACGGTCGTCAGCGCAAGGGTCATTTCGTCGCTGGGAAGGGACGGCCCTCGCCCGCGGTGATGGTGGT
TAGCAAGATGAACAAAGATGCGCAGATGAGAGCAGCGATTAACCAAAAGTTAATAGAAACTGGAGAAAGA
GAACGCCTCAAAGAGTTGCTGAGAGCTAAATTAATTGAGTGCGGCTGGAAGGATCAGCTAAAGGCACACT
GTAAAGAGGTAATTAAAGAAAAAGGACTAGAACACGTTACTGTTGATGACTTGGTGGCTGAAATCACTCC
AAAAGGCAGAGCCCTGGTACCTGACAGTGTAAAGAAGGAGCTCCTACAGAGAATAAGAACATTCCTTGCT
CAGCATGCCAGTCTTTAAGGGTGAATTAGAATGTGTTGGTCTGTGGATTTATTTCTGAAAGTAAAACTTG
CCATAATTAGAAATTGATTTCCCAAATAAAGTCCTTTTTTGTATGATGGTATACAGTTTTCAGTAATGTA
TACATTGTATTGATTTTTTCCCTAAATGTGTTATTTTAATAAATATCTCATGAATGAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4012930 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4012930 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11309 same embryo
 EMAGE:11307 same embryo
 EMAGE:11308 same embryo
 EMAGE:11312 same embryo
 EMAGE:11311 same embryo
 EurExpress:euxassay_006635 same experiment
 MGI:4824567 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS