EMAGE mouse gene expression spatial database

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EMAGE:11490

Serpinb6b serine (or cysteine) peptidase inhibitor, clade B, member 6b ( MGI:894688)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006907. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006907_01	euxassay_006907_02	euxassay_006907_03	euxassay_006907_04

euxassay_006907_05	euxassay_006907_06	euxassay_006907_07	euxassay_006907_08	euxassay_006907_09

euxassay_006907_10	euxassay_006907_11	euxassay_006907_12	euxassay_006907_13	euxassay_006907_14

euxassay_006907_15	euxassay_006907_16	euxassay_006907_17	euxassay_006907_18	euxassay_006907_19

euxassay_006907_20	euxassay_006907_21	euxassay_006907_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11490_wholemount_strong.wlz

11490_wholemount_moderate.wlz

11490_wholemount_weak.wlz

11490_wholemount_possible.wlz

11490_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11490_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

regional

strong expression: see section 07 08 09 14 15 16 17

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10

submandibular gland primordium mesenchyme

moderate

regional

moderate expression: see section 16 17 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 07 08 17 18

eyelid

moderate

regional

moderate expression: see section 05 06

naris

moderate

regional

moderate expression: see section 14 16 17

naso-lacrimal duct

weak

regional

weak expression: see section 09 10 11 18 19 20

aorta

strong

regional

strong expression: see section 09 10 11

umbilical artery embryonic part

strong

regional

strong expression: see section 13 14 15

rectum

moderate

regional

moderate expression: see section 12

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 12 16 17 weak expression: see section 15

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 16 17

ovary

strong

regional

strong expression: see section 05 06 07 08 17 18 19 20 21

left lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 moderate expression: see section 02

right lung

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9945
Entity Detected:	Serpinb6b, serine (or cysteine) peptidase inhibitor, clade B, member 6b ( MGI:894688)
Sequence:	sense strand is shown >T9945 AGATTCTGCTTGCTGACAGCCTGAACCTGGGGTTCCAGGCTTACCATCATGGATCCACTGCTGGAAGCAA ATGCCACTTTTGCCTTAAACCTTTTGAAAACACTGGGTGAAGACAGCTCCAGAAATGTACTTTTCTCACC TATAAGCGTCTCCTCAGCCCTGGCCATGGTCTTCATGGGGGCAAAAGGAACTACTGCAAGCCAGATGGCT CAGGCACTGTCTTTGGATAAATGCAGTGGCAAAGGAGGTAGAGATGTCCACCAGGGATTCCAATCACTTC TCACAGAAACGAACAAGACTGGCACACAGTATGTGCTCAGAACAGCCAACAGGCTCTTCGGGGAAAAGAC TTTCGATATCCTAGCTTCTTTTAAAGATTCCTGCCGCAAGTTCTATGAAGCAGAGATGGAAGAGCTGGAC TTTAAGGGTGCTACAGAGCAGTCCCGACAGCACATCAACGCCTGGGTAGCCAAAAAGACAGAAGATAAAA TTACAGAGTTGCTATCTTCGGGTTCAGTAAATTCAAACACTCCACTGGTCCTTGTGAATGCCATCTACTT CAAAGGAAACTGGGAGAAGCAGTTTAACAAAGAGGACACTCAAGAAATGCCTTTCAATGTCACCAAGGAT GTGGTGAAACCTGTGCAAATGATGTTTCAGAAGTCTACCTTTAAGATGACCTATGTGGAAGAGATATCCA CCAACATTCTGTTGCTTCCCTATGTTGGCAATGAGCTAAACATGATCATCATGCTTCCAGATGAACACAT TGAACTAAGTATGGTGGAAAAGGAGATAACTTATAAGAAATTCATAGAGTGGACGAGGCTGGACAAGATG GAAGAAGAAGAGGTGGAGGTTTTCCTCCCAAAGTTTAAGCTGGAGGAGAACTACGACATGAAAGATGTCC TGTGCAGGCTGGGCATGACTGATGCCTTTGAGGAGGGCATGGCAGACTTTTCAGGAATAGCATCTAAAGA AGGCTTGTTTCTGTCCAAGGTCATTCACAAGTCCTTTGTGGAGGTCAATGAGGAGGGCACAGAGGCTGCA GCTGCCACTGCAGCCAATATAGGTTTTAGGTGTATGGTCCCCTACTTCTGTGCCAACCACCCTTTCCTTT TCTTCATCCAACACAGCAGGACCAGTGGGATTGTGTTCTGTGGTCGGTTCTCCTCCCCATGAGAAAGGAT GCTGTGGTGTCAGGCCACTGCCTTTGCCCATTCTTTTTCTCATGATTTGTCTGCATCAGTAAATGTTGTG ACAATTCTGCACTTGGTAGCACACACCTGAAAAGCTAACATTTAGCAGATGAAGGTGAGAGAATCAGAGA TCATGCAGGGCTAGCCTCAACTGCATAGCAAGTTCAAAGCAAGCCTGGGCTACACTAGACCATTAAACAC AAAACCAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30276988 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30276988 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11492 same embryo
	EMAGE:11494 same embryo
	EMAGE:11491 same embryo
	EMAGE:11495 same embryo
	EMAGE:11493 same embryo
	EurExpress:euxassay_006907 same experiment
	MGI:4827994 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source