EMAGE mouse gene expression spatial database

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EMAGE:11593

Ftsj3 FtsJ homolog 3 (E. coli) ( MGI:1860295)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006943. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006943_01	euxassay_006943_02	euxassay_006943_03	euxassay_006943_04

euxassay_006943_05	euxassay_006943_06	euxassay_006943_07	euxassay_006943_08	euxassay_006943_09

euxassay_006943_10	euxassay_006943_11	euxassay_006943_12	euxassay_006943_13	euxassay_006943_14

euxassay_006943_15	euxassay_006943_16	euxassay_006943_17	euxassay_006943_18	euxassay_006943_19

euxassay_006943_20	euxassay_006943_21	euxassay_006943_22	euxassay_006943_23	euxassay_006943_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11593_wholemount_strong.wlz

11593_wholemount_moderate.wlz

11593_wholemount_weak.wlz

11593_wholemount_possible.wlz

11593_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11593_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

telencephalon mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 07 11 12 13 14 15 18 19 20 21 22

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 10 11 12 15 16 17 18

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 15 16

pons mantle layer

moderate

regional

moderate expression: see section 07 17

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 18

trigeminal v ganglion

strong

regional

strong expression: see section 17 18 19 moderate expression: see section 03 04 05 06 07 08 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 17 moderate expression: see section 08

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 17 18 weak expression: see section 08 19

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 16 17 18 moderate expression: see section 07

ovary

moderate

regional

moderate expression: see section 06 07 08 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7074
Entity Detected:	Ftsj3, FtsJ homolog 3 (E. coli) ( MGI:1860295)
Sequence:	sense strand is shown >T7074 GTTGAAAACCTGCGTAGTTAAGGGCTATCTAACGTCTGGAGTGAGTGATACTCCCCCATCCCGCCATGGG CAAGAAAGGCAAAGTAGGCAAGAGCCGGCGGGACAAGTTCTATCACTTGGCCAAGGAGACTGGTTATCGT TCGCGCTCTGCCTTCAAACTGATCCAGCTCAATCGCCGCTTTCAGTTCCTGCAGAAAGCCCGGGCCTTGT TGGACCTGTGTGCTGCGCCTGGCGGATGGCTGCAGGTGGCTGCCAAGTTTATGCCTGTATCCAGTCTTAT TGTAGGAGTGGACCTCGTTCCAATCAAGCCTCTCCCCAATGTGGTGACTCTCCAGGAGGATATCACAACA GAACGCTGTAGGCAGGCCCTGAGGAAGGAGCTGAAGACCTGGAAAGTTGATGTTGTGCTTAATGATGGGG CTCCCAATGTGGGGGCTAGTTGGGTCCATGACGCTTACTCCCAAGCTCACCTAACTCTGATGGCTCTGCG TTTGGCTTGTGATTTTTTGGCCCGTGGTGGTTGTTTCATCACAAAAGTCTTTCGTTCCCGAGACTATCAG CCCTTGCTGTGGATCTTCCAGCAGCTGTTCCACCGTGTCCAGGCCACGAAGCCCCAAGCCTCTCGCCATG AATCTGCAGAAATCTTTGTAGTCTGTCAAGGATTCCTGGCTCCGGACAAGGTTGATGCTAAATTCTTTGA TCCCAAGTTTGCCTTTAAGGAGGTTGAAGTTCAGGCTAAAACTGTTACTGAGTTGGTGACAAGGAAGAAG CCAAAGGCTGAAGGCTATGCCGAAGGTGACCTTACTCTTTATCACCGAACCTCAGTTACGGACTTCTTGC GAGCTGCCAATCCTGTTGACTTCCTCTCCAAGGCCAGCGAAATCTCAATAGATGATGAAGAGTTGGCACA GCACCCAGCTACAACTGAAGACATTAGAGTATGCTGTCAGGACATCAAAGTGCTGGGGCGAAAGGAACTT AGGTCTCTTCTGAATTGGAGAACAAAGCTTCGGCGATATGTGGCTAAGAAGCTGAAAGAGCAAGCAAAGG CATTGGACATCAGCCTCAGCTCAGAAGAAGAAGAAGAAGGTGATGAAGAAGAGGCAGTAGCTGAGACAAA GCAGGCGCCAGAGGAAGAAGAAGAGAGAGAGGAAGAACAGCTTAATCGGACTCTGGCAGAGATGAAAGCC CAAGAAGTGGCAGAACTCAAGAGGAAGAAGAAGAAGTTGCTTCGTGAGCAGAGGAAGCAGCGGGAGCGTG TCGAGCTGAAGATGGATTTGCCTGGGGTTTCTATTGCAGATGAGGGGGAGACAGGCATGTTCTCCCTGCG AACCATCCGTGGCCAGCAGCTATTAGAGGAAGTAACACAAGGAGACATGAACGCTGCAGACACCTTTCTG TCTGATCTGCCAAGGGATGACATCTATGTGTCAGATGCTGAGGACGATGATGATACTTCTCTGGAAAGTG ACCTGGATCCAGAAGAGCTGGCAGGAGTCAGGACACACTCGGATCTGAAGGAGCAGAAGTACTTGCGGTT TACTAAAGTAGATGACAATAAAGAGGAAGGAGGAGAAAATCCATTGCTGGTACCACTGGAAGAAAAGGCA GTACTGCAGGAAGAGCAAGCTAGCCTGTGGTTCTCCAAGGACGGCTTCAGTGGGATAGAGGATGATGCTG ACGAAGCCCTGGAGATCAGTCAGGCACAGCTGCTGTACAAGAGCCGTCGGAAGGAGCAGCAGCCAACAGA TCCACCTCCACCACCTACTAACTTGAAGACTGAGAAGAAATCTCCCCAGGGCCAGAATGAGGTCCCTAAG GAGACAGAAGCCATCTTGGGGACAGAGGCTGTCACTGACCCTGGAGGGGAGGAAAGAGGCAACAGCTCAG ATAGTGACAGCAGCAGTAGTGAGGATGAAGATAGCTGGAAAGTGTCCCGTGGTGTGAAGCGAGGCCGTGG CTCTAAGGCAGATGAGGATGGGTTTGAGGTGGTGCCTATTCAGGACCCAGTGAAATACCGAATTCTGGAC CCTGAAGGCCTTGCTCTAGGTGCTGTTATTGCCTCTTCAAAAAAGGCAAAGAGAGACCTCATAGATAACT CGTTCAACCGGTATGCTTTTAATGAAGAGGAGGGAGAACTTCCTGAGTGGTTTGCACAGGAAGAAAAGCA GCATCGGATACGCCAGCTGCCTGTTGATAAGAAGGAGGTGGAACATTACCGAAAACGCTGGCGGGAAATC AATGCACGGCCCATCAAGAAAGTAGCTGAGGCCAAGGCCAGAAAGAAACGGAGGATGCTGAAGAAGCTGG AACAGACCAAAAAGAAGGCAGAAGCTGTGGTGAATACAGTGGACATCTCAGAACGGGAAAAGGTGGCACA GCTGGCCAGTCCAGTACATACTGCTGGACTTGGCATAGCAGTCATTGTTAGAGTTTTTCATCTCGCCTTT AAGGGTGTGAACCACAATAATCTCCCAGGATGCAAGCCAAAAGGTCACGCAAAGATAGTTGTTGCTCGAA TGAAGAAGGATCAGAGAGCACAACAACGGAAGGAACAGAAGAAAAAACACAAGCGCAAGTGAGCAGAATT GTCTGGAAAGACTGAAGGACAGAGAGGGAGGTGTACAGTTCTCTCTGGAGGCTTTGTGGGATTGGGAGAG CTGCTACAGAGGAGTACCATGAAAGCACTGAGGAAACATTTCTTCATCGTGTTACACTGACAACCCACAT CTTGGCTTGTGATGGATCAAATCTTTTATGAAGCCGTCAGGGGGACTCAGTATTGCTGGCCTTTGTTGTA GCCTGTGGTTCCTTCGGAGTCCTCTCCCACCCTTTTCCAGTATTTGAATAAAAAGATAAAAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4012961 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4012961 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11592 same embryo
	EMAGE:11594 same embryo
	EMAGE:11595 same embryo
	EMAGE:11591 same embryo
	EurExpress:euxassay_006943 same experiment
	MGI:4824941 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source