EMAGE mouse gene expression spatial database

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EMAGE:11976

Camk2b calcium/calmodulin-dependent protein kinase II, beta ( MGI:88257)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009572. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009572_01	euxassay_009572_02	euxassay_009572_03	euxassay_009572_04

euxassay_009572_05	euxassay_009572_06	euxassay_009572_07	euxassay_009572_08	euxassay_009572_09

euxassay_009572_10	euxassay_009572_11	euxassay_009572_12	euxassay_009572_13	euxassay_009572_14

euxassay_009572_15	euxassay_009572_16	euxassay_009572_17	euxassay_009572_18	euxassay_009572_19

euxassay_009572_20	euxassay_009572_21	euxassay_009572_22	euxassay_009572_23	euxassay_009572_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11976_wholemount_strong.wlz

11976_wholemount_moderate.wlz

11976_wholemount_weak.wlz

11976_wholemount_possible.wlz

11976_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11976_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 21 22 23 24

submandibular gland primordium mesenchyme

moderate

regional

moderate expression: see section 09 10 11 12 18 19 20

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 19 20 21

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 09 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 20 21 moderate expression: see section 18 19 weak expression: see section 08 09

trigeminal v nerve

strong

regional

strong expression: see section 10 11 18 19

spinal cord

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 12 18 weak expression: see section 13

cervical ganglion

moderate

regional

moderate expression: see section 10 18 weak expression: see section 19

thoracic ganglion

weak

regional

weak expression: see section 14 15 16 17

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 17 18 19

neural retina

strong

regional

strong expression: see section 02 03 04 moderate expression: see section 01

nasal cavity olfactory epithelium

strong

spotted

strong expression: see section 13 moderate expression: see section 09 10 11 12 14 16 17 18 19 20

vomeronasal organ

moderate

spotted

moderate expression: see section 17

tongue muscle

moderate

spotted

moderate expression: see section 11 12 13 14 16 17 weak expression: see section 15 18

stomach

moderate

spotted

moderate expression: see section 06 07 08 09 10 11 12

midgut

moderate

spotted

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 22 23

kidney calyx

moderate

regional

moderate expression: see section 08 09 10 11 19 20 21 22

tail paraxial mesenchyme

weak

regional

weak expression: see section 12 13 14 15 16 17

extraembryonic component

moderate

spotted

moderate expression: see section 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9355
Entity Detected:	Camk2b, calcium/calmodulin-dependent protein kinase II, beta ( MGI:88257)
Sequence:	sense strand is shown >T9355 ATCGCCACCGCCATGGCCACCACGGTGACCTGCACCCGTTTCACCGACGAGTACCAGCTATACGAGGATA TTGGCAAGGGGGCTTTCTCTGTGGTCCGACGCTGTGTCAAGCTCTGTACCGGCCATGAGTATGCAGCCAA GATCATTAATACCAAGAAGCTGTCGGCCAGAGATCACCAGAAACTGGAGAGAGAAGCTCGGATCTGCCGC CTGCTGAAGCATTCCAACATTGTACGCCTCCATGACAGCATCTCTGAAGAGGGCTTCCACTACCTGGTCT TCGATCTGGTCACTGGTGGGGAGCTCTTTGAAGACATCGTGGCAAGAGAGTACTACAGTGAAGCTGATGC CAGTCACTGCATCCAGCAGATCCTGGAAGCTGTTCTCCATTGTCACCAAATGGGGGTCGTCCACAGAGAC CTCAAGCCTGAGAACCTGCTTCTGGCCAGCAAATGCAAAGGCGCCGCAGTGAAGCTGGCAGACTTCGGCC TGGCCATCGAGGTTCAGGGAGACCAGCAGGCATGGTTTGGATTTGCGGGAACGCCAGGCTACCTGTCTCC CGAGGTCCTTCGGAAGGAGGCCTACGGCAAACCTGTGGACATCTGGGCATGTGGGGTGATCCTGTATATC CTGCTGGTGGGCTACCCACCTTTCTGGGATGAGGACCAACACAAGCTGTACCAGCAGATCAAGGCTGGGG CGTATGATTTCCCATCCCCTGAGTGGGACACCGTTACTCCTGAAGCCAAAAACCTCATCAACCAGATGCT GACCATCAACCCTGCCAAGCGCATCACGGCCCATGAGGCCCTGAAGCACCCATGGGTCTGCCAACGTTCC ACCGTGGCCTCTATGATGCACAGACAGGAGACTGTGGAATGTCTGAAGAAGTTCAATGCAAGGAGGAAGC TCAAGGGAGCCATCCTCACCACTATGCTGGCCACACGGAATTTCTCAGCCAAGAGTTTACTCAACAAGAA AGCAGATGGAGTCAAGCCCCAGACAAACAGCACCAAAAACAGCTCGGCCATCACCAGCCCCAAAGGATCT CTCCCTCCTGCTGCCCTGGAATCTTCCGACAGCACCAACACAACCATAGAGGATGAAGATGCCAAAGCCC CCAGGATCTCTGACATCCTCAACTCAGTGAGGCGGGGCTCAGGGACCCCAGAAGCTGAGGGCCTCCCACC AGTGGGCCCCCCACCCTGCCCATCTCCGACTCTCCCTGGCCCCTTGCCCACCCCATCCCGGAAGCAGGAA ATCATCAAGACCACAGAGCAGCTCATTGAGGCCGTCAACAATGGGGACTTTGAGGCCTATGCGAAAATCT GTGACCCAGGCCTGACCTCATTTGAGCCTGAAGCTCTGGGCAACCTGGTCGAAGGGATGGATTTCCACAG ATTCTACTTTGAGAACCTGCTGGCCAAGAACAGCAAGCCGATCCACACCACCATCCTGAACCCGCACGTG CACGTCATTGGCGAGGATGCGGCATGCATCGCCTACATCCGCCTCACACAGTACATCGATGGCCAGGGCA GACCCCGTACCAGCCAGTCCGAAGAGACCCGTGTGTGGCACCGCCGCGACGGCAAGTGGCAGAATGTACA TTTCCACTGCTCGGGCGCTCCAGTGGCCCCGCTGCAGTGAGAGCTGCGCCTGGTTTCGCCGGACAGAGTT GGTGTTTGGAGCCCAGCCGCCCTCGGGCGCACGGCCTGCCTGTCGCATGTTTGTGTCTGCCTCGTCCCCT CCCCTGGTGCCTGTGTCTGCAGAAAAACAAGACCAGATGTGATTTGTTTTTAAAAATAAGATGATGACAA CGACAACCACACGCAAACCAACAAACAAAATTTTGGCATCGGATGAAATGGAAAACAAGCAAACAAACAA AAACCTAAAGAAAGGAAAAAGCTGTAAAAATCTGGCATTTGTGGGGCCACCCCACCCCCAAGCTCCACTG TGTCTGTTGTGCTCCTGGCTTTGGGGACCCTCAGGGCGTGAATGTGCCCATGCCGACCGTGTTAGTCTGT AGGTACCGCACTCACCCCATACCTTGGGCGTCTGCATCCTCCCTCCCATGCATGGGCCCCACGTGCTAAC CTGGCTGTTGTGGTGTCAGGTGTCTTGGGAAGGGCTGGGGGCTGCCTCCTTTCCTGCTAGTTGTGCCTGA GAGTTGCTGCTGCTCCTGCTTTCCCCTACCTGTCCCTTGTCCCGTCCTGTGAAGGGCTGGGAGGGTGCTC CTCTAGCCAAGGACCTACCTTTGCCCCATGGAGTGTGTAGAAGCTTGTCCCCGAGGTCATCAGAAAAGAA CCTGGGCTCTCAGCCCCGGTTCTTTGGGGAGTGAGGTATGGAGGTACGGATCTTGCCTCCAGACCTCCTG CCTGGCAGGGGTTGGTTAAGAAGGCCTGCTGGCTGCAGTGCCTACCCATGTAGCTATGAAGAGAGGACTG GGCAGAGGATGGCCTCCTTCCTGTTTGGGGTACTTGTCCCTTCCCTGCCACCAGCAGCTGAGCAACAGTG GCCCACCCCTGGCCTGGGAAGTTCCTCTCTGAAGTTGGCAGCAGACCCTAATGAAGATGGAGAACCTGAG ATACTGGACGGATACTGGGAACAGGCCAGGCTGCGTCTAGGGTGTTTGTCCACTCAGCTTGGAGCCTGGG TGTTCGAGACTTGGTTAAATGTTGCAGAATGTGGTAAAGAGGGGCCAGAAGTTGGGTTCTAGACTTCTGG GCAATGCAGGTCCATACAAGTGTTTGATGAAATGGGAGTGCTTTCTTGACCTGGGCCTAGAGAAGAGCAT ATGCCACCTAGAGGCATGTGGAAGGCCAAAATTGGTTAGGGCTGTATTGTGTGGGGCTCTTCCAGGTAGG CCAGAGACACAGCTTAGAGAGAAGGCAGGCTGGGGGCAGAGATATATGGCTGTGGGGCCTGGATACCAGT GGGTGGGTGGAGGGCCCATCAGGACAGTGTGGCACCTCCAAGGGCCCAGTGGTGCTCCTAGGTGCCGGCT GCCTGGACTCGTTGTGGGTGGCCCTGGCCCTTGGTGACTGAGGGAATCATTAAGGCTGGACATGTTACAA AGTCAGGCTAGGGAGATATAGGTCTGAAGCCGGGTTGGGTGGGGGTGGTCAGAGGCTTTGACTGCAGAGG TTCATGGGCCTCCACCCCAACGCTGACCACTTTAAATTCATGTCATTTTCAACCAGGATTTTCTTTATCT TTTACTACAAATCAAGCCAAGGGAGGGGCACGAAGTGGGGAACGGGACACAGGATCCTAAACTCCAAGGG GACTGTCCACCTACAGACACTCAGTGGATACCACCTTTCATCCACACTGTGCCCAGGACAGCTGTTCCCT ACCCATGGACACAGGGTAAACATCTGCCACCCATGGACACAGGGTAAACATCTGAGTGGCCCAAGGCCAT GGAACAGTGGCAGGGCTTGCCATGGACAGTGTGAGGCCCTGTGGCCTGCCACCCTGGCATCCTGGGGTCT CCTTTCCTCTCCCACCCACCTTGTTATCTCCACAAAGCTGCCTGTCTGGGATAATTTGGGGATTTTTTTT TTCTGGGGGACAATTCTTTTGCATGACCCTCTAAAGAGCAGGACACTCCTGCTCTAACTGGCTAGGTGTT CGCAGGTGGTGGCGTCGACCGCTGGCCAGTGCTGTAGTGTGTTACTGCCTGCAGTACTGGTGGCCCTCCT CTCTTCTCTCTTTTTGCTGAGTTCCACTGTCTTTTCTTTCTGAGCCTTGTAGGTGTACAAAACTTATTCT TATTTTGTTCTGTCTCGGGAAACTGCAAATAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6851995 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6851995 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11979 same embryo
	EMAGE:11978 same embryo
	EMAGE:11977 same embryo
	EMAGE:11975 same embryo
	EurExpress:euxassay_009572 same experiment
	MGI:4823603 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source