EMAGE mouse gene expression spatial database

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EMAGE:12099

Dlx1 distal-less homeobox 1 ( MGI:94901)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008426. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008426_01	euxassay_008426_02	euxassay_008426_03	euxassay_008426_04

euxassay_008426_05	euxassay_008426_06	euxassay_008426_07	euxassay_008426_08	euxassay_008426_09

euxassay_008426_10	euxassay_008426_11	euxassay_008426_12	euxassay_008426_13	euxassay_008426_14

euxassay_008426_15	euxassay_008426_16	euxassay_008426_17	euxassay_008426_18	euxassay_008426_19

euxassay_008426_20	euxassay_008426_21	euxassay_008426_22	euxassay_008426_23	euxassay_008426_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12099_wholemount_strong.wlz

12099_wholemount_moderate.wlz

12099_wholemount_weak.wlz

12099_wholemount_possible.wlz

12099_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12099_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand

moderate

regional

moderate expression: see section 01 02 03 04

foot

moderate

regional

moderate expression: see section 04 05 06 07 21 22 23

vibrissa

moderate

regional

moderate expression: see section 05 06 07 08 20 21 22 23 24

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

cerebral cortex mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 21 22 23 24

telencephalon mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 21 22 23 24

inner ear

strong

regional

strong expression: see section 02 03 04 23

cochlea

strong

regional

strong expression: see section 08 09 18 19 20

utricle

strong

regional

strong expression: see section 05 06 07 19 20 21 22

retina

strong

spotted

strong expression: see section 01 02 03 04 05 24

neural retina

strong

spotted

strong expression: see section 23

anterior naris epithelium

strong

regional

strong expression: see section 13 17

external naris epithelium

strong

regional

strong expression: see section 12 13 18 19

naso-lacrimal duct

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 19 20 21 22 23

esophagus

strong

regional

strong expression: see section 13 14 15

tongue muscle

moderate

regional

moderate expression: see section 10 12 13 15 16 17 18 19 weak expression: see section 11 14

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11

midgut

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

lower jaw skeleton

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 18 19 20 21 22 23 24

lower jaw incisor

strong

regional

strong expression: see section 11 12 13 16 17

upper jaw skeleton

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 18 19 20 21 22 23 24

upper jaw incisor

strong

regional

strong expression: see section 12 13 17 18

tail paraxial mesenchyme

strong

regional

strong expression: see section 13 15 16 moderate expression: see section 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9421
Entity Detected:	Dlx1, distal-less homeobox 1 ( MGI:94901)
Sequence:	sense strand is shown >T9421 CGCAGCCCATTGTGCGTCCCGCTCCGCGCGCTCTGTTGCAGAGGAGCCCCGGCCGGGACGTGTGGACCGG ATTCTCCCCAGGCCGGGCCGCGTCGCCCGCTCTAGTCCAGCCGAGCCCGAGCTTCTCGGACCAATCCCCG GTGATTATGCAAGAGAGCCGACCAATCAGCTCCACCAGCTCATGAATATTTATGACCTTGGCTGAGTCAA AGCTTTGAACCGAGTTTGGGGAGCTCGGCAGCATCATGCTTAGACTTTTCAAAGAGACAAACTCCATTTT CTTATGAATGGAAAGTGAAAACCCCTGTTCCGCTTAAATTGGGTTCCTTCCTGTCCTGAGAAACATAGAG ACCCCCAAAAGGGAAGCAGAGGAGAGAAAGACCCACACCCAGACCCCGCGAGAAGAGATGACCATGACCA CCATGCCAGAAAGTCTCAACAGCCCCGTGTCGGGCAAGGCTGTGTTTATGGAGTTTGGGCCGCCCAACCA GCAAATGTCTCCTTCTCCCATGTCCCACGGGCACTACTCCATGCACTGTTTACACTCGGCGGGCCATTCG CAGCCCGACGGTGCCTACAGTTCGGCCTCATCCTTCTCCCGACCGCTGGGCTACCCCTACGTCAACTCGG TCAGCAGCCACGCGTCCAGCCCCTACATCAGTTCCGTGCAGTCCTACCCCGGCAGCGCCAGCCTCGCCCA GAGCCGCCTGGAGGACCCAGGGGCGGACTCCGAGAAGAGTACGGTGGTGGAAGGCGGCGAGGTGCGCTTT AACGGCAAAGGGAAAAAGATCCGTAAACCCAGGACAATTTATTCCAGTTTGCAGTTGCAGGCTTTGAACC GGAGGTTCCAACAAACTCAGTACTTAGCTCTGCCTGAGAGGGCGGAGCTGGCGGCCTCCTTGGGACTCAC ACAGACGCAGGTCAAGATATGGTTCCAGAACAAACGCTCCAAGTTCAAGAAGCTGATGAAGCAAGGCGGG GCAGCTCTGGAGGGCAGCGCGCTGGCGAACGGCAGAGCCTTGTCTGCCGGCTCCCCACCGGTACCACCCG GCTGGAATCCGAACTCCTCATCTGGGAAGGGCTCCGGCAGCAGCGCTGGCTCCTACGTCCCCAGCTATAC ATCATGGTATCCCTCAGCGCACCAAGAAGCTATGCAGCAGCCTCAACTGATGTGAGGTTATCAGTTCGCA CTCACCCGCTTCCTGCCCGTCTCCCTTGGCCCGGCCCCTCCCGCCTCCAGGTTCATCCACCTTTTCGCAC GCCCTGGGCCAGGAGGAGAAGAGCCCACGAGCGGGAGCAGGGGTGGGGGTGGGGAGCTAAAAATAAAAGA AAAAGGAACAGTGAAGGCAGAGGGCTTGCTGCTTCTCGGAGCCCCTAAAAGTCTGACTCAGCGACCTTTC AGCCCGAATCTGGTTTGGGCCACGGTGAACAGCAGAGTGGCCTCAAAGCGCAGGCACAGCCACTGGAGAC AGCCAGTGCCACAAGATAACCCGCTGGACCGGACCAGACTCTCATCTTTGTGAGACTATCAGGAAAAACA AATACGTAGGAGGGGGAAATACGCTCTTTTTTGATTCTGTCCGTCTTTGTCTCCTTTTTGCACTGTCTGT GCGCCGAAGTCAAGGTCCTCGTGTGTCTGCTGTCCTCATTCTACGACCTCTCCAAAAAGTCACCAGGTCT GAGCCACACCGGTCTGCCGGCAGCCCATCCACCGAGTCCTGGACCACTTTTTCCTGAATGGTCTTCTTCC GGGCCCTGCTTGACCATCTATGTAGCTTGTTTGGGAAATGGGGGAAATCAGAGGGAGGGGGGTTAATTTA TTGAAAAACAGACTTCATCTGACGCTGAGTGTTGGCCAATTCCAAATTCTCTGGGACATGGAGTGGGCGC TGTCCAAACATACTATTTACCTTAAACACACCAGAAGAGGGAACAAGGACAGGAAAGTGACTTTTAAGGT GCACAGTAACTTTTGTACTTGGCTGAAATGCAGAAAGGGAAAACAGGATGCCGTGCATTTTACATGCTCC AAATTATTAAAAATAACAACAAAAGAAAGACCGAAGTAAGGAAGTAAGATATGTAAAACCAATAGGATGT TGTCAAAAACGTTTGGACAAGAACACCTGGCCAAGAAGAAAAATCATACTTTGGGCAGCAAGAGAGAGGA CCAATGAGCCTTGGAGAGAAAATAATTTGTCCTTTATGTAAAAGCACCCCAATTCCAGGTCGCTTTAAAG TAAGACACAAGCAGCGGCTCGACCACAGAACACAAGTCATCACCCTGAATGCACGGGCTCTGGTGCAAGA TCTAATTGCTGTACATATTATTGTTGTTATTATTATTAATTATTATTATTATTGTTCTGTAAACATGTTG CACAAGCTTAGCCTCTTTCCGTTCTGTTGTGTGTGGCTGTAAACCCGATGCTTTGTGAAATGAGAATCTT GACATTTTACTTGTGAAATTTGGAAAATGTGACCGATTGAAATCCACCGTGTTTTGTGTTCTCTATGTCA AAGTTTAGTTTTATATTGAGAATGTTAACTTATTGCTTTGTATCTTGGGGGGGGGGCGAACTTTGTAAAT AAGTTATAAAGTTTCTTTGAGACAGTAAAATTATGGTTTCAAGAAAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30360273 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30360273 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12095 same embryo
	EMAGE:12097 same embryo
	EMAGE:12096 same embryo
	EMAGE:12098 same embryo
	EurExpress:euxassay_008426 same experiment
	MGI:4824327 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source