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EMAGE:12294

Frmd5 FERM domain containing 5 ( MGI:2442557)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12294 EMAGE:12294 EMAGE:12294 EMAGE:12294 EMAGE:12294
"Pseudo-wholemount" of euxassay_010946. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010946_01 euxassay_010946_02 euxassay_010946_03 euxassay_010946_04
EMAGE:12294 EMAGE:12294 EMAGE:12294 EMAGE:12294 EMAGE:12294
euxassay_010946_05 euxassay_010946_06 euxassay_010946_07 euxassay_010946_08 euxassay_010946_09
EMAGE:12294 EMAGE:12294 EMAGE:12294 EMAGE:12294 EMAGE:12294
euxassay_010946_10 euxassay_010946_11 euxassay_010946_12 euxassay_010946_13 euxassay_010946_14
EMAGE:12294 EMAGE:12294 EMAGE:12294 EMAGE:12294 EMAGE:12294
euxassay_010946_15 euxassay_010946_16 euxassay_010946_17 euxassay_010946_18 euxassay_010946_19
EMAGE:12294 EMAGE:12294 EMAGE:12294 EMAGE:12294 EMAGE:12294
euxassay_010946_20 euxassay_010946_21 euxassay_010946_22 euxassay_010946_23 euxassay_010946_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12294Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12294_wholemount_strong.wlz
12294_wholemount_moderate.wlz
12294_wholemount_weak.wlz
12294_wholemount_possible.wlz
12294_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12294_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
peritoneal component
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08
foot mesenchyme
moderate moderate
regionalmoderate expression: see section 07 08 09 24
adrenal medulla
strong strong
regionalstrong expression: see section 09 10 11 12 19 20 21
pancreas
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 18 19 20
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
vibrissa
weak weak
regionalweak expression: see section 07 08 23 24
facial vii ganglion
weak weak
regionalweak expression: see section 05 06 07 22 23 24
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 08 09 21
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 05 06 07 08 10 11 19 20 21 22 23 24
vagus x ganglion
weak weak
regionalweak expression: see section 10 21
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 08 09 10 weak expression: see section 04 05 06 07 19 20 21 22 23 24
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 11 19
spinal cord
strong strong
regionalstrong expression: see section 12 13 14 15 16 17 18 19
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 12 13 18 19
cervical ganglion
strong strong
regionalstrong expression: see section 11 19 moderate expression: see section 20
thoracic ganglion
strong strong
regionalstrong expression: see section 14 15 16 17 18
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 11 12 13 14 19 weak expression: see section 09 10 18 20 21
retina
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 16 17 moderate expression: see section 15 18 19 20 21
vomeronasal organ
strong strong
regionalstrong expression: see section 13 14 16 17
heart atrium
moderate moderate
regionalmoderate expression: see section 07 08 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 09 10 11 12
heart ventricle
moderate moderate
regionalmoderate expression: see section 08 13 14 15 16 17 18 19 weak expression: see section 09 10 11 12
esophagus
moderate moderate
regionalmoderate expression: see section 15 17
stomach
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 weak expression: see section 02
stomach mesentery
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11
rectum
moderate moderate
regionalmoderate expression: see section 16
midgut
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17 18 19 20 21 22 23
rest of midgut mesentery
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17 18 19 20 21 22
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 12 13 14 weak expression: see section 17 18
lower jaw molar
moderate moderate
regionalmoderate expression: see section 08 09 22 weak expression: see section 21
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 12 13 14 weak expression: see section 17 18
upper jaw molar
moderate moderate
regionalmoderate expression: see section 08 09 22 weak expression: see section 21
bladder
moderate moderate
regionalmoderate expression: see section 15 16
kidney calyx
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 19 20 21 22 23
kidney pelvis
moderate moderate
regionalmoderate expression: see section 21
collecting duct
moderate moderate
regionalmoderate expression: see section 12
urethra of male
moderate moderate
regionalmoderate expression: see section 15 16 17
left lung
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13
right lung
moderate moderate
regionalmoderate expression: see section 14 15 16 17 18 19 20 21 22 23 24
trachea
moderate moderate
regionalmoderate expression: see section 14 15 16
tail paraxial mesenchyme
moderate moderate
regionalmoderate expression: see section 14 15 16 17 18 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8431
Entity Detected:Frmd5, FERM domain containing 5 ( MGI:2442557)
Sequence:sense strand is shown

>T8431
GGAGGCGGTGGTGGCTCTGTAGAAGGAAGAAGAGTGGGGCGGGAGGAAGCAGCGGTGGCGATCCGGTCAG
GGTGATGGTGCAGGTGCTAAGGGCCGGCGCGGAGCTGCCGCGCTGAGGGGCGACGGGTCCGGAGTCCGCA
GCGCCGCCGCGTCGCTCCCGAGGACGGGCGAGCGGGCGGGAAGATGCTGAGCAGGTTGATGAGCGGCAGC
AGCAGGAGTCTGGAGCGCGAGTACAGCTGCACCGTGCGGCTGCTGGACGACAGCGAGTACACCTGCACCA
TCCAGAGAGATGCCAAAGGCCAGTACCTGTTTGACCTCCTTTGCCACCACCTGAACCTACTTGAGAAAGA
CTACTTTGGGATTCGTTTTGTGGACCCAGATAAGCAGCGGCATTGGTTGGAGTTTACAAAGTCAGTGGTG
AAGCAGCTGAGATCCCAGCCTCCGTTCACCATGTGCTTCCGCGTGAAGTTTTACCCTGCCGACCCCGCTG
CCCTGAAAGAGGAAATAACCAGGTACTTAGTCTTCCTTCAGATCAAAAGGGATCTCTACCACGGCCGTCT
TCTCTGTAAAACATCGGATGCCGCCTTGTTAGCGGCCTATATCCTTCAAGCGGAGATTGGGGATTATGAC
CCGGGAAAGCACCCTGAAGGCTACAGCTCCAAGTTCCAATTTTTCCCGAAACATTCAGAGAAACTGGAAA
AGAAAATTGCTGAGATTCACAAGACCGAACTCAGTGGTCAAACACCAGCAACATCAGAGCTGAATTTCTT
AAGAAAGGCCCAGACGTTAGAGACCTACGGAGTGGACCCTCACCCATGTAAGGATGTGTCGGGAAATGCT
GCGTTTCTGGCCTTCACTCCTTTCGGGTTTGTTGTTCTTCAAGGAAACAAGAGGGTCCACTTCATTAAAT
GGAATGAGGTGACCAAGCTGAAATTTGAAGGAAAGACTTTCTATTTATATGTAAGTCAGAAAGAGGAAAA
AAAGATTATTCTCACATATTTTGCCCCAACTCCAGAAGCCTGCAAGCACCTCTGGAAATGTGGAATTGAG
AACCAAGCCTTCTACAAGCTGGAAAAGTCAAGCCAAGTCCGCACAGTGTCCAGCAGCAATTTGTTCTTTA
AGGGGAGCCGGTTCCGATACAGTGGCCGGGTCGCAAAGGAAGTAATGGAGTCCAGTGCCAAGATCAAACG
GGAGCCGCCAGAAATACACAGAGCAGGGATGGTCCCTAGCCGCAGCTGTCCTTCTATAACCCATGGCCCA
CGACTGAGCAGCGTCCCCAGGACACGAAGAAGAGCTGTGCACATCTCTATCATGGAAGGCCTTGAGTCGC
TACGAGACAGTGCCCATTCCACCCCAGTGCGGTCCTCTTCCCATGGGGACACCTTCCTGCCTCACGTGAG
AAGCAGCCGGGCAGACAGCAATGAACGTGTCGCTGTGATAGCGGATGAGGCCTACAGCCCCGCAGACAGT
GTGCTGCCCACCCCTGTAGCCGAGCACAGCCTGGAGCTGATGCTGCTCTCCCGGCAGATCAACGGGGCCA
CGTGCAGCATTGAGGAGGAGAAGGAGTCGGAGGCCAGCACCCCGACTGCGACAGAGGCGGAGGCCCTGGG
AGGAGAGCTGAGGGCCCTGTGTCAGGGGCACGGCGGGTCAGAGCAAGAACAGAGAGTGCATCTGAAGGGA
CCACAGCTACAGCAGCAGCAGTGGAAGGGGTGGGGTAAGTCTGTCCCCTTAGACTAGAGCCTAGTGAACA
CCTGAGTTGTATTTAATGTTTTGCTTTTCTTTCTGAAGAACCTCAAGACTTTTAGTGCTTCTGGGGTGTC
AAAGTGGTGCCAGAGTGTGCGGTCAGAGATGTCCCACAGCACAGTTACCAGAGACCTGTGCTGAGTCACA
CGAGAGCCTGCAGAGCCGCAGGGAGCTGTGCTTGTCTTGTTACAGTATGCCTCTCCCCCCAAAAGACTAT
TTCAAGCTCTATTTTAACAGCAAAATGTTATTTTCCTATCTATTCCTATTTTGTTTTCACTTTATGAATC
AAAAGATGCAAGAGAGAGACAGATCTGAGGAAGGCATCGAGAAGGAGCAGCGGCCTTGGAAGGGACCAGG
AAGCACTAAGCAGGACCTCTAAGCAATGCCTACAGGTGACAGAATTTGACCATGGCAGAGACCAGCACTT
ACTATAGGAATGCCACCCTTAGGGTCAACCCCTGCTCCTGGAAACAAGGTCTAAGAGCACTTAGTGTCGT
CCCTCACAGCAGCATACGTGTACACACACACACACACACACACACACACACACACACACGCACGAGCTGC
AGTCCCTAAGCCTCTACACTTCTTACCACCAATAACACACAAGAGCATGTTTTCATCTCAGGGAGACCGC
AAGTCATCTGAGGCCCAAGGACTCACGTAAAGGACAGTCAAAGGGCACACTCCTGGGACCAGAAAACAGT
GGAGCGGAGCAGCCCCAACAGATTGGGCGGTAGAATATTTGAGCTTCCCTTATTTGTTGCCTGGGGTAGA
ACCATGTCCACATTCAGAATATGACAGCCATTAAAAGCAACAACAACACCCAAAAACAAACAAATGGGGT
TTTGAAGATGCTGGGAGGGCATTTACATAATTTTAAAGAAAGATGACATCCTTATCTAGACCAGAGGTCA
ACAAACCTCTTTTAATAAAATTTTGATTTTGTGGACCATGCTCTGCCACCCTAGCACGCCTCTGATAATG
CATGCATAGAAGAACATGGCTTGCTCTGAGAAAATTTTATAAAAACATAGGGCTGAGGATGTGTAGCTCA
GAGGGTAAAGTATTTTTCTAACATGTATAAGGCCCTGGATTTGACTTCAAACAATACACAAAACCAGGTG
TGGTAGCATATACCCGTGGTCCCAGCATTCAGCAGGGAGACGGGAAGATCAGAAGTTCAAGGCAATCCTC
AGTCAGTGAGTTAATCCCAGTCTGGGATACATGAGGTGTCTCAGACCACCGGCTTCCCACTCCCTAAAAA
CAAACCTAAATCTGCAATATGCTGAGAGACTGGTCCCCAGCAGTACGATGCAAACCCTGGTCTACACAGG
GCCAGCTTCCATTGCTGTTTTGTGTAAGCTACCCAGAACCATTTCCCAATGGGCAGGGTGCTCCAACAAC
CAACCAGCAGTGATGTGTGTCATCTGATACCAAGATCAAGAAGTACTGCCACTGTGAACAGCGGTGACAC
TATGAACGGGTCCAGGCCTATCTGATCATTTCCGTACCAGCAACAATTTGTGTGAGATCTAAAACATATA
CCACATGACAAGACAAACTGAGGGGATCCTAGTAAAGACCAGAGGTTACTTAAAAGCAATGAGCCACTGC
TGCTTGTCAACTGCATAACCAAGGTGTGTACTCAGCACAGGACTGACAGCAAAGGCTACTGGGAAACAGG
GCTGTGTGAACTGGGATTTCTATTCTTATGCAACATAGCAGATTGCCTTCTAGAGTGGTGCTCCTACCTC
AGTCTGTAGAACACAGTGTCTATGCAGTGAGTGGTAGTCTCGCCTCTGACCTTTCATAGCCTCGAGAGAG
GCAGCCTGCCATTGCCTGGTGTGTTTCTTGTGTCTAGAGTCCTAACAGTGCTGCGATCCGTGCCATTTCC
AACATCTGACCCAGAATCTTCAGGTCATTTTCTCCCTCAAGGCTTAGCCCAGAAAATAAGGACAGAAAAG
GCTCCCTTCGATTCGGTTTCCAAATAGTTTTCAATAGTTCAGTAACAAACCCCCTTTACTGGTGACTAGT
GTTTTTCATCATTTCCCCATCATTACAAAAGTTTCTGTTTTCGTCTGGTTTTGCCGTATTCTGTGTCTCT
TCTTTCCTCTCAGAATTATTGATCTCACTGTAGAAAAGCAGATCTGCCTAGCATTATTGATCAGAGGAAA
TACTTACTGAATAGTGGACTGCATCTTTAAGCAGTAGAGGATAAACTACCTACAAATGTGAATTTCTTAG
TTTCACTTAAAAGTATACACCTGTTAACCTTTCGTGTGCCAAATCTAAGTTACGTTTTCCTTCAAAGCAA
TGTACTCTTTTCTACATATGTAGTATGTAGTTAGCATAAAATCATTGGTGCCATGAAGATTATTTTTAAA
TCCAATAAATATTTAAATACCGTAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5698263 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5698263 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12298 same embryo
 EMAGE:12293 same embryo
 EMAGE:12297 same embryo
 EMAGE:12295 same embryo
 EMAGE:12296 same embryo
 EurExpress:euxassay_010946 same experiment
 MGI:4824924 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS