EMAGE mouse gene expression spatial database

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EMAGE:12295

Nuak1 NUAK family, SNF1-like kinase, 1 ( MGI:1925226)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010978. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010978_01	euxassay_010978_02	euxassay_010978_03	euxassay_010978_04

euxassay_010978_05	euxassay_010978_06	euxassay_010978_07	euxassay_010978_08	euxassay_010978_09

euxassay_010978_10	euxassay_010978_11	euxassay_010978_12	euxassay_010978_13	euxassay_010978_14

euxassay_010978_15	euxassay_010978_16	euxassay_010978_17	euxassay_010978_18	euxassay_010978_19

euxassay_010978_20	euxassay_010978_21	euxassay_010978_22	euxassay_010978_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12295_wholemount_strong.wlz

12295_wholemount_moderate.wlz

12295_wholemount_weak.wlz

12295_wholemount_possible.wlz

12295_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12295_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 05 06 07 22 23 moderate expression: see section 08

cerebral cortex mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 14 16 17 18 19 20 21 22 23

telencephalon mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 21 22 23

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 22 23 weak expression: see section 06 07 08 09 10 20 21

dorsal root ganglion

moderate

regional

moderate expression: see section 12 19 20 weak expression: see section 11 13 18

neural retina

strong

regional

strong expression: see section 01 02 03 moderate expression: see section 04 weak expression: see section 05

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 16 17 18 19 20 21

vomeronasal organ

moderate

regional

moderate expression: see section 16 weak expression: see section 14

aorta

moderate

regional

moderate expression: see section 13 14 15 16

mandible

moderate

regional

moderate expression: see section 10 11 20 weak expression: see section 05 06 07 08 22 23

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 14 17 18 19

lower jaw molar

moderate

regional

moderate expression: see section 08 21

maxilla

moderate

regional

moderate expression: see section 10 11 20

upper jaw incisor

moderate

regional

moderate expression: see section 13 14 17 18

upper jaw molar

moderate

regional

moderate expression: see section 09 21

metanephros

strong

regional

strong expression: see section 09 10 11 12 13 19 20 21 22

axial skeleton

moderate

regional

moderate expression: see section 14 15 16 17

orbito-sphenoid

moderate

regional

moderate expression: see section 23 weak expression: see section 05 06 07

clavicle

strong

regional

strong expression: see section 12 19 moderate expression: see section 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9405
Entity Detected:	Nuak1, NUAK family, SNF1-like kinase, 1 ( MGI:1925226)
Sequence:	sense strand is shown >T9405 CAATGCTGCTGCGGGGGCGACCTTGAACGCACCCCGGTTTCCCTCGGTGGGGACCACCCCGAGCCCCTGT GCCGGGACCTGCCCTCTCCAACTCGGCTCGCCCTGCGCTTGGTCATGGAAGGGGCGGCAGTGTCCGCGGC AGGGGATGGCCCTGCTGTAGAGACCGGCTTGCCGGGCTCTCCGCTGGAGGCGGTGGCCGGGGCGACTGCA GCCCCAGTGGAGCCCAGGAAGCCGCACGGGGTGAAGCGACATCACCACAAACACAACTTGAAGCATCGCT ATGAGCTGCAAGAGACCCTGGGCAAAGGTACCTACGGCAAAGTCAAGAGAGCCACCGAGAGGTTTTCAGG CCGAGTGGTTGCTATAAAATCCATCCGTAAGGACAAAATTAAGGACGAGCTAGACATGGTTCACATCAGA CGAGAGATTGAGATCATGTCATCCCTCAACCATCCTCATATCATCAGTATTTATGAAGTGTTTGAGAACA AAGATAAGATTGTGATCATCATGGAATATGCCAGCAAAGGAGAGCTGTATGATTACATCAGTGAGCGCAG GCGCCTCAGCGAGAGGGAGACCAGACACTTCTTCCGGCAGATTGTGTCTGCCGTGCATTACTGTCACAAG AATGGTGTGGTCCACCGGGACTTGAAGCTGGAGAACATACTGCTTGATGACAACTGCAACATTAAGATTG CTGACTTTGGGCTCTCCAACCTGTACCAGAAGGACAAGTTCTTGCAAACATTTTGTGGGAGCCCACTCTA TGCGTCGCCCGAGATTGTCAACGGCAGGCCTTACCGAGGGCCAGAGGTGGACAGCTGGGCCCTGGGTGTC TTGCTTTACACTCTCATTTATGGAACGATGCCCTTCGATGGCTTTGATCACAAAAACCTCATCCGGCAGA TCAGCAGCGGAGAGTACCGGGAGCCCACACAACCCTCAGATGCTCGGGGACTCATCCGGTGGATGCTGAT GGTGAATCCCGATCGCAGAGCCACCATCGAGGACATCGCCAACCATTGGTGGGTGAACTGGGGCTACAAG AGCAGCGTCTGTGACTGTGATGCCCTTCCTGACTCTGAGTCTCCACTCTTGGCACGGATCATCGATTGGC ATCACCGTTCCACGGGGCTGCAGGCTGAGGCTGAAGCCAAGATGAAGGGTCTGGCCAAACCCGGGGCCTC TGAGGTTGTGTTGGAGCGGCAGCGGTCACTGAAGAAGTCCAAGAAAGAGAACGACTTTCCCCAGTCTGGC CAGGACTCCGTACCTGAAAGCCCATCCAAGCTGAGTTCCAAGAGGCCCAAGGGCATCCTGAAGAAACGGA GCAACAGCGAACACCGCTCCCACAGCACTGGCTTTATCGAAGGCATTGTGAGCCCTGCCTTACCATCCCC TTTCAAAATGGAGCAAGATTTGTGCCGGACTGCCATCCCCCTCCCCAGCTCCCCCGAGGCAGATATGTCA GGGAAGCTCAGCCTCAAACAGTCTGCCACGATGCCCAAGAAAGGCATCTTGAAAAAGACCCAGCAGAGAG AATCTGGCTACTATTCATCCCCAGAGCGCAGCGAGTCCTCGGAACTGCTGGACAGCAACGATGTGGTCAT CAGTGGTGGCCTCTCGTCCCCTCCCCCAGACCCAGCCAGGGGAACTTCCCACAGCCTGTCCTGCCGGAGG AAGGGCATCTTAAAACATAGTAGCAGGTACTCAGATGGTGGCACAGACCCTGCCCTCACCCGCCCTGAAA TGCCCACACTGGAATCCTTGTCACCTCCCGGTGTCCCCTCCGATGGCATCTCTCGGAGCTACAGTCGCCC GTCCAGTATCATCAGTGATGACAGTGTCCTGTCCAGCGACTCTTTCGACTTGTTAGAGCTTCAAGAGAAC CGGCCAGCCCGCCAGCGCATCCGCAGCTGCGTCTCTGCTGAAAACTTCCTCCAGCTTCAGGACTTTGAGA CACCACACAACCGGCCCCGGCCCCAGTACCTGAAGCGGCTGGCAGACAGTAGCTTCTCCCTTCTCACTGA TATGGACGATGTGACTCAGGTGTATAAGAAAGCGCTGGAGATCTGCAGCAAGCTCAACTAGCCCTTCAGG GCACAGAGGATGGGATGGGTGGGTGGGTGAGGCCAAGGGAGAAGGGGAACAGAACTCAGCCTGAAGGCTG GTTACCTCTTTGCTGGTCAAGGCAACGGATTGGGAAAGGACTAGTAATGTCTGGCCTGGCCAGTTTACAC AGCCTTGAGTCAGCACCTAAAAGGGATAGAAACCATTCTTTACCAGTTCTGCCTGTGCCAGTTAGAATGG GGGTCCTAACTGGCATTCCCAGGTGACATCTCACGGGGGAGGTGGTGTTGGCCAGCAAGAAATGGGAAGC GGCATTATGGGGAAGGGTGTGTTTCCTAGAAGTCATCTAGATGTTTCTGGAGGAGGGGAAGAAAGACTGA GACCATCTGCTTTGGATGGATCCATTAGCAAGCCTGGTCTGACTACACAGGGGCTAGCAAGACTCCAGCA ACCTGAGTGTGAATTGGTGATCAATTGGTAATGTCTGTACTGTGGTCTGAGTTTAGTAGACTAGGCACAC CAGGGAGCATTGAGTGAGTACCAATAACTGCCAGGGAATCAGGACCGGAGGCAACAGCATACCTGCCCTG GCTAAGCCCTGGGAGTTAGGTTAAAGCAGGCTGTTTTAAAGAAAAACAGTTAACTCTTTCATGGCACAGC ATGACATGTGTTAATTTGCTCCTGGACCTTGAGTAGAAGACAGGGCTTGCTTAGAGAGTGAATATGCTGA ACTCTGCTACCGAAATAAGTCTGGTGGTGGTGACTTTCTGGTTTTCTGGGATCTGCCTCTTCTTTCTCCT CTCTCTTGCCTTGTTCTTCTTTAGTGGGATCTTCTGAAGGTGAGCAGGAATAAAGATGGCAGTGTGAGAG CCCAGGGCTTTGTGTGTAATGGGGCACAGAGCTGCTGGGGCCTTTGCTCAGTGAATGGACTGACGGTGAA GCATGCCAGCAAGGGGCGACTTAACACTTTCTTAAATATCTCAGTATTAACTGGAACCCCGCCTCTTGGC TTCTCACCTGCACAAGGACAGGATGCTACTCTGAGTCTCCAAAGCATCACTGGCTCAGCCATAGGCCCCA TTCACCCCAAGTGTGGAATCTTGACTGTAGAAGAGTTGCTTCTAGGGAGTTAGTATGTAGGTGAAATATG TGGCCGAGGGAAAGCCCAGTTTGACAGAAGGTTCTTGTCTCGCCAGGCCTTAGTATTCTGCCTCGTGGTT TTGATCTCCCTCATTTTGGGGGAATAAACAAGTTCCATTCATCCATCCTTCCATCCATCCATCCATCCAT CCATCCATCCATCCACAGAATAAGATGTTGAGAGAGCAGAGTGTGGGGGGAGGAGGCTTAGGGACGTTTC CTCGGGAGCCATCCAGATGGCTCTGGAGGAGGCAGAAAGGAGGTGTATGGGGTGACAGGATGCTTAAAAA TGTTCCAGCTAGTTATCTAATCTCTGAACACTAAGATTACAGGTGATGTCATCCCCTCAGAGGGGACCCT GGGGACACTGGGGGCGGGGGTGGGGGGGATTGAAGTGGTCTTTCTGGGCTGACCACGGAATCCACTGGCT TAGTCAAATTAGAATCCTTCTTCTAAGGTGGCTGCCTTGTGGGAGCCTCTCAGACAGGGTTGGGGGAGGA TGCATCCGTCACTGCACTGAGGACAGTTGTCAGGGAGATGCTGCTCTATACAGCAGAAGGCTGCTTTGCA TCACTGTGTTTGGGCCCCGCGCTTTCCTTTGGGCTGAAGTGAGCAGGATGTAGTGGGAAGCTGCTTCGAT TCTTTTCCTTCAGTTTCACCTCCTACCTGAACTGTGGCAGGGGCTAAAAGGGAGGAAAATCTATAGGTAG AGAAGTGGCTCTGGCGCTTGGAGGTGTATAGACGGCAGCATGTAACCTCTGAATCCTCCCGCTCCCTGCC TCCTCTCGTGAACAACCCCGCCTGCTGCTACACTGCTGGCCCCTGAGCAGGCAGCTGGTCTCGGGTGTAG CAGCCATTGCTGGTTGATGGAATTTTTCCCTTTTGCCAGCTGATTGTGTCTGTTTAGTATTCCCCACCAT GCAGTTGACTCCATTAGGAAAACCACGCCCTTCGGCTTTCTCCCCTTCTCGATCACCAGGGTCACCAGTG TGTTCTCTTTTGGCGGGACTCTCAAAGACAAGGGGTTGGGGAGGGGAAACCAAATGTTTCACACCAAGAA CAGAGGCTCTCGGGTGTGGGGTGTGTCCTCCGTTCCAGGGCTGACACTGAGTTGAGTCCAGGTCTGCCTT GATCTGGAGGTCTCAGGCACCTGCAGTGGCTATGAGGGCTGCTTCCCACGGGTGGAGGCTGGAGACGTGG CTGAGTGGTTTCCCAAGGCCCTCCCCGCCCCCCTCAGTTCTGATACCTGCTTCTAGCTGAGATGCTGACT GTCCCTGCAGGAAATGAGTTAGATCAGAGAATAAAGCCCTAAAAAAAGCAGTTTCCCAATGGAGCCCTGT TATCCCTGCCAGTCAGTCCCCAGCCAAAGAAGGGATGCCTGACTCATTTCCGCGGAGTCCCCAGTGAGAG ACACAGATGACCCCATGGGCAGAGTGGGAAAGTCGCTGCGTGAGCTGTCCTCGTCCTTCCTTTGCTTCTA GGGCACTTCTATTCTTCGTTTACACATCTGCCAAAGGGGGTGGAGTTGTACTTCGTTTTACAGGTAAATT TCAAGGCACAGTCAGTTTTTAAGAAAGTGCTCCAAGACCCTAGGCAACGTGACAGTTCTCAGTTCCCTCT GAATTTTAGTCTCTGCTACCTGGTGCTCTTTACCAAAGGAGCTCGCTGCAGGGTGAAATTCAGGCGTGTT CTGCCCAGGCCTGCTGTCTTGAGTACAAATGTGAATGATGGACTGACTGCTTGTTGCCAAACTGGAAATG TTTTGTAGGGATTTACTGGCATGGTATCATTCCTAGGAAGAAGAAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30355405 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30355405 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12298 same embryo
	EMAGE:12294 same embryo
	EMAGE:12293 same embryo
	EMAGE:12297 same embryo
	EMAGE:12296 same embryo
	EurExpress:euxassay_010978 same experiment
	MGI:4826823 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source