EMAGE mouse gene expression spatial database

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EMAGE:13032

2510009E07Rik RIKEN cDNA 2510009E07 gene ( MGI:1919440)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013634. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013634_01	euxassay_013634_02	euxassay_013634_03	euxassay_013634_04

euxassay_013634_05	euxassay_013634_06	euxassay_013634_07	euxassay_013634_08	euxassay_013634_09

euxassay_013634_10	euxassay_013634_11	euxassay_013634_12	euxassay_013634_13	euxassay_013634_14

euxassay_013634_15	euxassay_013634_16	euxassay_013634_17	euxassay_013634_18	euxassay_013634_19

euxassay_013634_20	euxassay_013634_21	euxassay_013634_22	euxassay_013634_23	euxassay_013634_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13032_wholemount_strong.wlz

13032_wholemount_moderate.wlz

13032_wholemount_weak.wlz

13032_wholemount_possible.wlz

13032_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13032_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 21 weak expression: see section 05 06

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 06 07 08

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13 14 moderate expression: see section 10 15

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 09 15 16 17 weak expression: see section 08 10 11 14

telencephalon ventricular layer

strong

regional

strong expression: see section 04 05 18 19 20 moderate expression: see section 08 09 13 15 16 17 21 weak expression: see section 06 07 10 11 14

rest of cerebellum marginal layer

strong

regional

strong expression: see section 03 04 05 06 07 08 12 13 21 22 moderate expression: see section 09 10 11 17 18 19 20

midbrain ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 moderate expression: see section 08 09 17

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 09 20 weak expression: see section 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 09 20 weak expression: see section 18 19

dorsal grey horn

weak

regional

weak expression: see section 10 11 12 13 14 15 16 17

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 15 16 17 18 19 20

neural retina

strong

regional

strong expression: see section 01 02 23 24 moderate expression: see section 03

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9221
Entity Detected:	2510009E07Rik, RIKEN cDNA 2510009E07 gene ( MGI:1919440)
Sequence:	sense strand is shown >T9221 GAGAGGAGAAAGAAGGGAGGAGGGAGCAGGGCGCAGAAGGGAGCAGGGCGCGGGGAGGCAGCAGGCCTGG CCGGGTCGCTGCTCCCTCCCCGGGCGGCTCCTCGGCAGCGGGTGTTCGCCGCGCCTCCAGCCCGCAACCC ACCCTCGCCCTGCCCGGCCCGCGCCTCTGCCCTGTCCTAGGCTCCCGGCCCGGCTCTTCAACCGGAGAGA TGAGTGCGGCCACGGCGCCCGAGCGCGGCTGGAAGAGCGAGAAAGTGGACGAGGCGCAGGCCCTGGCGCG GAGCTGCGCCGCCCGCAGACCCGACTTCCAGCCGTGCGACGGGCTGTCCATCTGTGCCACGCACAGCCAC GGCAAGTGCTTCAAGCTGCACTGGTGCTGTCACCTGGGATGGTGTCACTGCAAATATGTGTATCAGCCTA TGACCCCCGTGGAGCAGCTCCCAAGCACAGAGATCCCTGCAAAGCCCCGAGAGCCGACTAACACCATTCA AATCTCCGTCTCGCTCACTGAACACTTTTTGAAATTTGCGTCTGTCTTCCAGCCTCCGCTTCCCCCTGAC TCGCCAAGGTACTGTATGATTTCCGATCTCTTCATCGACAACTATCAGGTTAAATGTATTAATGGGAAGA TGTGCTATGTGCAGAAGCAGCAGGCGCCCCACTCCCAGAAGATGAGCCCTGAGGAGGTCTCTGCACACGA TGCCTTAATTTCTAAAGAGAGTGACACTCCGAAACTGGGTCACTGCTCCTCCCCTTCCGGTTCCGAGGAC TCGGGGATCAACGCCATTGGGGCTCACTATGTGGAATCCTGTGATGAGGACACAGAAGAAGGGGCGGAGC TGAGTTCCGAAGAAGATTACAGCCCTGAGAGCAGCTGGGAACCCGATGAGTGCACCCTGCTCTCCCCATC GCAGTCGGATCTGGAAGTGATTGAAACGATGGAGACCACTGTGTAGGCAGCTGGGAACTGCGGCCGGAGG TCCACCCTGAGCCTCTTCTGTTCTTTGATTGGACGAGTCCTTTTCCAATACTCTTGGCCTTTTCCAAGGC ATATTACTAACAGTCGCAGTCCAGTGGTCTGCTTAGTGTCCCCTTATATTACCTTGTAACGACATTGTTT CCTTTTGTATCTTGCTTTCTATGTAGCATCTGGTGTCATGAGTTGTGACCCAGCCTTAATTTCACTGAGA ACTTTGAGAGCAGTGGTGACCACAGCGTATCCAATGGAAGAGCTACCCAGAGTGGAAGTAACCATATTTT ACCAGTAGCCTGTCCTACGTGATGAAGGCTCAGCTTCCCTTTGGCGTGTCATGTGACACCTCTGTCCTGT TGGTTTCCGGGTGGGGGAGCAGCTCACTTCTGGAAACAGTGTTGCAGCCACTGTTCCCTCCCTTGCTCTT CTGTTTGCCACCCACATTGGCTCAGGACAAGAGTGGCTTCGGCTACTTCTAGGAGGTCCTTGGAGGCCCT TTTGACTCCTGGTGATGGCCATGTCACAGCATCTGTGACTGAAAGGGTCTCTGGGGTTTGTGTCCAGTCC AGCTGGGATTCAGGAGGACTCAGTCCTGGTTCTCACCTCGAAGGGGCCACATTTTAGTACAGGACACAAG TCAAGCAGCTCGCTGAGCCAGGAGGATAATGGGATCAGGGGAAGGAGAGTGGAGTTGGCCTGGGCTGGCT GAGAATGTGGCTGCCGTGTCTGAGCTGACCCCAGGTGGATTCTACAGCGCCCACTGCCCTTGATTGTTTT GCAGTTCAGGGAGAAGCATTTAGGTTTCGTAGGTATTAGAGTTAGGAGGTAACACATCTGACATCTTAAG GAAAAAATACACACTAGTTTTAGTTAGCATGAAAATGCTAAGAGACAGAAGTCCTTTAGGGATTAGGAAT GAATCTAATAGGTTTGATCAATCCTGCTTCGTTTGGTTGTATGAAGCATTAGAATGGCTTAGATGAGAAA TGGACTAGAATTTTGTATGATGCATGGATTTTATGCAAATGCAAAAACAGTTGAAATAGCTCACTGCTGA GTCAGGAAGATGTTTTATGTAACACTTCTTCACTTTACTCCACTGGAATCCACACACATTGTGTGGATTC TCTCTCACACACAAAATAGTGAGTAATTCTTATGTAGCTTTCTTCAAAACTCTGTTCCTAATTTGCTATT AACTGTGTATTCTCATGTGATCTTATTATCTGCCATTTCCCCAAATATATCTGTGAGGCATAGTACAAAT CTGGGACTTTTAAAAATATACTGTCATAAAACAAGAGTCCCAGGGCTGCAGGGAGACCTGCCTCAGATCC TGGCTGTCTGAGCAGGCACTGTGGCCTTGCTGTGTGATCAGCATCTCTCTACATCCCTCTGTCTCATTAG TGTGTACTGCAGGCAAGGAGAGCATGCTCCCTAGGCCTCCTCATTTGGGGGCTCTTCACTATGTACGCAA GGAAGAATCTGCTTCCCTAGTTGGATATGGAGTCCCAGTGCTTACTACTAGAACCTTCTTTAGGCTGCCT ATCCCCAAACACCCAGTGTTCAGAAGGCAGAATATATGCCTTCCTCTGGAAAGCATTAGTTAAAACGCCC TGTGTGAGATGAGAATGTAGCAACTCCTCCCTCTCCCCACATACTTAAGGACTCACACCAAGGACCAGCA GACACAGGGAGTGAGGATTCACCAAAGACCAGCAGGTACAGGGAGGGCAGGTGGGAGCCACCATATCTTG GCTGTGGGGTCATTCTGCTGGCCTGGGATGGTCTGGTGACACCTGGTGGGCCCAACTGAAGGTCGTAGTG CCTCACTGTCATGGAAAGGAAACACATGAGCCACATATCCCTCATCCTGCACTCGTGGCTGTGATGGGCA GCAGCGGCACCATCCTCAGACCCTGAGGTGGAGAGGCAGACACACGGCAGGACTTACTCCTGCACCTTTC CTATCTACTTTTTTGCTACAGGGCCACTGCCAAATACTAAACTAACTGGGATCTAGAACCCGGGCCCCAA AGCCATGAAAACACACGGTCATTGCCAAAGTTCTGAAGGCTAGCTGTGTGTTGGGCTTGGTCCCTTTATT AGTATAACATCTGTGACTTCTGTCCAGGGTCTTGTTTTCTAAACCCCTGAAACTGCCACTCAGACTTGCC ACCATTGTGATGGGATTTTTGGATTTAATAACAATTGGTGATCACATTAAATATTTGCACCAACTTTATA ACTACATTCACTAAGCTTCACTTGCCGTTTTATAAAATTGGACACGTTCCCTTGCATTCTTGGATCTCCA AACAGCCTTTTCTAAAGAAGCACAGCCGAGAGGTTTTTGGTTTTCTCCTCCTAACAGACTTCATACCAGT AACTGTCTGAAATATGACTAAGCCAGAATCATTTCTCTATGCTTGCAGTCTGAAATCAGCTCAAGAGACT ATAAAAAAAATTGTATAGCTTAACCACAATTCTAGGCCTATTCTGTTCTTTGTTCTATGAAAGAATTTCT CTATAATCCTGATATTATAGTGCTGATTTGTATATGCGTATTCATAGGCACTTTAGAAATACTTAGACAC AACATGCACTGTAGGCGGTAAGCACAGTGGTGTTAAATAGGCTTTTGACTAACACTTGTACAGTGAGAGA GACAAAAGCTTGCAATCTGGACCTTATAGGATGCTTGCCTGGAGCTAGGCTTGGACCACGTGGATAGGTT TTTGTGGGATTCTGGCACTGCTACCACTTTTGAAAGGTTGTTGGGTACCAGGGTAATCCCACTAAATGTG GAGTATACTTTTCTTACCTAACCAAGATCTTAAGTTTCAAATGTGTCCATAGCTTTTGTTTTTTGTTTTT TGTTTTTCTGAGAAATGACAAAGCAATTAAAGTCCTTTTTGGGATAACTATTTGATATCTTTAAAAAAAA TGAACTTTAAAACCTGGGGTTTTTTTTGCCTTTAACATTTAAGGAATAATATGAGAATCTGAGGACAACC CTGAAGACAAAGTTGTTTTCCTAGAAGAAAAGACTAATGCCTCAAGGTGAACAGACTAAAACCTGAGCCA CAGAATCCAAACACAACACAGCACAACACAGTGTAATATGGAGCCACAATGTAACACAACACAACACAGT ATAATAACAAATTAACAACACAACACAGTATAACAAAATAACAACACATCACAGTGTAATAACAAAATAA CAACACAACACTGTATAATAACAAAATAACAACACAACACTGAGCCTCCGATCAGAAGCACATGGCTGTT TTCTGACAAGCCTTTAGGACATGATCTATGATCTGATTGTTTGTTTTACTTTTTAACCTCACCTGCTTGC CTGATTGAAGTAACTTGTTCTTCTAGGGTTGATCAGAACAAATTATTCTTATGCAAATTTGATAGCAACC AGACACTTTAGAAAGTCAAAATATCCATATATGCCTGTAGTGCAGATACTGCCATGTAAGGGGAAAAAAA AAGCACAAATTTAAGTAAAATACTCTTTATGTCATCTGCTCTTGATTGTTCCCAAATCCTATTTACAAAA CTTTATTGTCATGGTTTTGGCTTTTTACTTGAGGAAAATATTCTAACTGGTCTCTTTCCTTCCTGTTTGT CCAGCGGTGCTGTGAACTAGAACCGTGGGTAGAGGCCGGGTACCCTGGGGTTTGTGGAGAAGGTGTCACT GTGCCCCTTCTTTGTACTACACAGCCCAGAGCACCATCTTAAACCTCAGTATAAACACAAGGGCATTTCT CAAAATGAATTTGTTTTTAAATTTCACCTTGTCGTGACTATGGTACGGGAACACGGGTGTCCCAGAAGGG CTGGTGCAGTTCAGGGGGAGCTGCTTGCTGCGGAGGTAAGGCACCTGGAAGCCAGCCTAGGGTTTGGATG GCGCCTCCAGGGCACTGGGTCTGCACGTGGGTTAAGGCTGTCTGTCCTTTGCAGTAGTCACCTGCTGTGC TAGGTTCTTTTGAATCTGTTTTGAAGTTTAAGCACAATGTTGACCCTTCTGTAATTTCCTAAAATTACAT TGTTTTCCTAAGACAGCTCAACTTTAGGAAAGTACTGCTGTACAATAAACGTGGTTTCCTTTTGTACTAG AAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6833156 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6833156 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13033 same embryo
	EMAGE:13031 same embryo
	EMAGE:13030 same embryo
	EurExpress:euxassay_013634 same experiment
	MGI:4822674 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source