EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14126

Serpinf1 serine (or cysteine) peptidase inhibitor, clade F, member 1 ( MGI:108080)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001804. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001804_01	euxassay_001804_02	euxassay_001804_03	euxassay_001804_04

euxassay_001804_05	euxassay_001804_06	euxassay_001804_07	euxassay_001804_08	euxassay_001804_09

euxassay_001804_10	euxassay_001804_11	euxassay_001804_12	euxassay_001804_13	euxassay_001804_14

euxassay_001804_15	euxassay_001804_16	euxassay_001804_17	euxassay_001804_18	euxassay_001804_19

euxassay_001804_20	euxassay_001804_21	euxassay_001804_22	euxassay_001804_23	euxassay_001804_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14126_wholemount_strong.wlz

14126_wholemount_moderate.wlz

14126_wholemount_weak.wlz

14126_wholemount_possible.wlz

14126_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14126_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 24 weak expression: see section 22

mesenchyme

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 24 weak expression: see section 22

vertebral axis musculature

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 24 weak expression: see section 22

gland

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 24 weak expression: see section 22

visceral organ system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07

integumental system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 24 weak expression: see section 22

diencephalon meninges

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon meninges

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 24

hindbrain meninges

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain meninges

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

spinal cord meninges

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

cardiovascular system

moderate

homogeneous

moderate expression: see section 14 18

liver and biliary system

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 24 weak expression: see section 22

reproductive system

moderate

homogeneous

moderate expression: see section 12 13 14 15 16 17

skeleton

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 24 weak expression: see section 22

tail

moderate

homogeneous

moderate expression: see section 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4926
Entity Detected:	Serpinf1, serine (or cysteine) peptidase inhibitor, clade F, member 1 ( MGI:108080)
Sequence:	sense strand is shown >T4926 CAGAGTGCAGGCTGTGAGAGAAGCTGCCGCAACCACAGTTCCGGGATGCAGGCCCTGGTGCTACTCCTCT GGACTGGAGCCCTGCTCGGGCACGGCAGCAGCCAGAACGTCCCCAGCAGCTCTGAGGGCTCCCCAGTCCC GGACAGCACGGGCGAGCCCGTGGAGGAGGAGGACCCCTTCTTCAAGGTCCCTGTGAACAAGCTGGCAGCA GCTGTCTCCAACTTCGGCTACGATCTGTACCGCCTGAGATCCAGTGCCAGCCCAACGGGCAACGTCCTGC TGTCTCCACTCAGCGTGGCCACGGCCCTCTCTGCCCTTTCTCTGGGAGCTGAACATCGAACAGAGTCTGT CATTCACCGGGCTCTCTACTACGACCTGATCACCAACCCTGACATCCACAGCACCTACAAGGAGCTCCTT GCCTCTGTTACTGCCCCTGAGAAGAACCTCAAGAGTGCTTCCAGAATTGTGTTTGAGAGGAAACTTCGAG TCAAATCCAGCTTTGTTGCCCCTCTGGAGAAGTCCTATGGGACCAGGCCCCGGATCCTCACGGGCAACCC TCGAGTAGACCTTCAGGAGATTAACAACTGGGTGCAGGCCCAGATGAAAGGGAAGATTGCCCGGTCCACG AGGGAAATGCCCAGTGCCCTCAGCATCCTTCTCCTTGGCGTGGCTTACTTCAAGGGGCAGTGGGTAACCA AGTTTGACTCGAGAAAGACGACCCTCCAGGATTTTCATTTGGACGAGGACAGGACCGTGAGAGTCCCCAT GATGTCAGATCCTAAGGCCATCTTACGATACGGCTTGGACTCTGATCTCAACTGCAAGATTGCCCAGCTG CCCTTGACAGGAAGTATGAGCATCATCTTCTTCCTGCCCCTGACCGTGACCCAGAACTTGACCATGATAG AAGAGAGCCTCACCTCTGAGTTCATTCATGACATCGACCGAGAACTGAAGACTATCCAAGCTGTGCTGAC TGTCCCCAAGCTGAAGCTGAGCTTCGAAGGCGAACTTACCAAGTCTCTGCAGGACATGAAGCTACAGTCG TTGTTTGAATCACCCGACTTCAGCAAGATTACTGGCAAACCCGTGAAGCTCACCCAAGTGGAACACAGGG CTGCTTTCGAGTGGAATGAAGAGGGGGCAGGAAGCAGCCCCAGCCCAGGCCTCCAGCCCGTCCGCCTCAC CTTCCCGCTAGACTATCACCTTAACCAACCTTTCCTCTTTGTTCTGAGGGACACGGACACGGGGGCCCTC CTCTTCATAGGCAGAATCCTGGACCCCAGTAGTACTTAATGTCTCAGTGCTCTACAGAACCCCCAGAGGG AAGCTGATTATACATTCCAGGAAGGCGGCCGGTAGCTTCAGTGTAGCCTCTGCAATAAAAGAGCTTTTCC TTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5123884 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5123884 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14125 same embryo
	EMAGE:14129 same embryo
	EMAGE:14130 same embryo
	EMAGE:14128 same embryo
	EMAGE:14127 same embryo
	EurExpress:euxassay_001804 same experiment
	MGI:4828001 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source