EMAGE mouse gene expression spatial database

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EMAGE:14351

Atcay ataxia, cerebellar, Cayman type homolog (human) ( MGI:2448730)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004136. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004136_01	euxassay_004136_02	euxassay_004136_03	euxassay_004136_04

euxassay_004136_05	euxassay_004136_06	euxassay_004136_07	euxassay_004136_08	euxassay_004136_09

euxassay_004136_10	euxassay_004136_11	euxassay_004136_12	euxassay_004136_13	euxassay_004136_14

euxassay_004136_15	euxassay_004136_16	euxassay_004136_17	euxassay_004136_18	euxassay_004136_19

euxassay_004136_20	euxassay_004136_21	euxassay_004136_22	euxassay_004136_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14351_wholemount_strong.wlz

14351_wholemount_moderate.wlz

14351_wholemount_weak.wlz

14351_wholemount_possible.wlz

14351_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14351_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

not examined

regional

not examined expression: see section 01

diencephalon

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

facial vii ganglion

strong

regional

strong expression: see section 02 03 04 05 17 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 04 05 16

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 15 16 17 18 19 moderate expression: see section 20

vagus x ganglion

strong

regional

strong expression: see section 05 15

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 03 04 05 06 15 16 17 18

spinal cord

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

strong

regional

strong expression: see section 06 07 12 13

cervical ganglion

strong

regional

strong expression: see section 05 06 14

thoracic ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15

dorsal root ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

neural retina

strong

regional

strong expression: see section 01 02 03 23 moderate expression: see section 21 22

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16 weak expression: see section 17 18

tongue

strong

spotted

strong expression: see section 12 13 14

stomach

strong

spotted

strong expression: see section 08 09 10 moderate expression: see section 02 03 04 05 06 07

midgut

strong

spotted

strong expression: see section 08 09 10 11 12 13 moderate expression: see section 07 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5614
Entity Detected:	Atcay, ataxia, cerebellar, Cayman type homolog (human) ( MGI:2448730)
Sequence:	sense strand is shown >T5614 GAAAGGGAAAAGCCAGAGCCGCGGCAGGCCTGGGCTGCGATGGCAGGGGAAACAGTGACTAAAGGGGGAC AGGGGTGCTGCTACTAACCCACGGCGCCGCCTTCTACAGTTGGGCCGGACAGGTGTGCTGTGGCCACGTC GCCCTGGGTGACCTTCCTCAGATGTGGACTTGGCCCTGAGCATCCTTCCACCAGGCCCCTCGCCTGGGTA CCATCGAGAAATGCCCGCCTTTGTGTCCAAGTGACAGCGCAGAGGCAGCTTCCGCTACCGAGATCATCTT CTGGGTCACCCGAGTTTCCAGACCACTTCTCTTTCCAGCTCTCATGGGAACCACAGAAGCTACACTAAGG ATGGAAAATGTGGACGTGAGGGATGAATGGCAGGATGAGGATCTGCCCAGACCGCTCCCAGAAGACACCG GGGTGGAGCGGCTGGGTGGCGCAGTGGAAGACTCCTCCTCACCTCCCTCCACCCTGAACTTGAGCGGAGC ACATCGAAAGAGAAAGACGCTGGTGGCTCCAGAGATCAACATCTCCCTGGACCAAAGCGAGGGCTCTCTG CTGTCCGACGACTTCCTCGACACACCTGATGACCTGGACATCAATGTGGACGACATTGAGACGCCAGATG AAACTGACTCTCTGGAGTTCTTGGGAAATGGCAATGAACTTGAGTGGGAAGATGACACCCCAGTGGCCAC CGCCAAAAACATGCCTGGTGACAGTGCGGACCTGTTTGGGGACGGCTCTGCGGAAGACGGCAGTGCGGCC AACGGTCGTCTGTGGCGAACTGTCATCATAGGGGAGCAAGAGCATCGCATCGACCTGCATATGATCCGGC CCTACATGAAGGTGGTCACCCATGGAGGATACTACGGGGAAGGTCTCAACGCCATCATCGTGTTTGCAGC CTGCTTCCTGCCAGATAGCAGCTCCCCAGACTATCACTACATCATGGAGAATCTCTTCCTGTACGTCATC AGCAGCCTAGAACTGCTCGTGGCTGAGGACTACATGATCGTGTATCTGAACGGCGCCACGCCCCGGAGGA GGATGCCTGGCATTGGTTGGCTGAAGAAGTGTTACCACATGATTGACAGGAGACTGAGGAAGAATCTAAA GTCCCTGATCATCGTCCACCCCTCCTGGTTCATTCGCACTGTGTTGGCCATCTCCCGGCCATTCATCAGT GTCAAGTTCATCAGTAAAATTCAGTACGTGCACAGCCTGGAAGAGCTGGAGCGACTGATTCCCATGGAAC ACGTGCAGCTGCCAGACTGTGTCCTGCAATATGAAGAGCAGAGACTCCGAGCCAAGAGGGAGAGCACACG GCCACCGCAGCCGGAGTTCCTCCTTCCCAGGTCAGAAGAAAAGCCAGAGACTGTGGAAGAAGAGGACAGG GCAGCAGAGGCAACAGAGGACCAGGAAACTAGCATGTCCTGATCTACCCAGAACCTAGACATGGACAGAG ATTATTCCCAACATCATGTAACCCTCATCCGAAGCCCTGGGAGCCCCGCCTTCATCGAGCCTCCACCGCC TGAGGATGCCTGGCTCCACCATGTCCCCTGCACCCGGGTCCTCTCACTGCTTGAGTCCATTTCACTGTTT TATTTTGAAGAAGGCGGTATGAGCACATCTCGGAATGGAGCAGGTTCTTGACCCTTTCACAGTCTGGGCT TGTGGATGCCAAAGGTGGATGTGGGCAGTGCCACCCACGGTTACTCTTCCAGGCCTTGGCATCCCCAAGA TGCCCTTCTCTGTCCCAGCTAGGATAACACAGATATGCATTTTGGGCTGACCCAATGAGAGACCCTTTGC TCGGTTGCCACCTGTCCCTAGCTGGAGCCTCAGATGTCTGGTGGCCCTGGCTGCAGCTGCATCTGCTTCT CTGTGGAATGTGACCCACTGTCCCCTCTCCCTGCCACCCCAGGCAGGGTTGTGACTGCTGACCTCACATC TCCACCTGACATGCACTATTCTGCCATTTGACTGTCTTTGGGGGCCTTCAAGCCCATTGCACATGTACTG ATCAGCCTGGTGGTCAACAAACCCAACATTTTGAGCTCCTTTAAAGTAGATGTGACTCCAGATGTTAACT GACACCTCATTCCAAGGGAAGAAGTTGTATCCACTTTCCTATCCTCTGCCCAGACCAGACTCAGCCTCTG TGTCATCAAGTAGGAGACATTTGGTGTCTGAGAAGAAAGATGAAGATCCAGGGCTGTGGGTGCTAGAGGA TCTGGGGGACCCCCTAGGACAGTATATCCTGGTGGGTGGGATGCTGCGACCACCTGATCTCCAAGCTGGG CTCAAAGCACATGTCTGCCCGAGATGACTACATAGTCTAAGCTAATGAGTGTGCTGAGCCTCCTGTCCCC AGGGACACATAAGGCCAGATGACATTTGCAGGGAGGAGTCTCCCTGTGGTTCCCTAAAGACTTGAGTATC TCTATGTTAAGAAATCTTAGCACTGCGCAGTGGTGGCATATGCCTTTAATTTCAGCACTCAGGAATAGGA GGCAGGGGGATTTCTGTGAGTTCCAAGATAGCAAGGGCTACATAGTGAGACCCTGTCCAGAAAAAAAAAG AGGAAGAGAGAGAGACAGACAGAGAGAGAGAGCTCTGTGCCTCTCCCCCAAAGGTAGGTTCTATCCACCC CACTGTGAGGTGTCCCCTCTGACATGTTTCTTGCTTCTCGGCCTCTGATGGCCGCAGGACAGCAGCTTCC ACCTAGAGAGAATCAGAGTGTCCTGCCAATAACACCCATGGATCTGAGCTCTGCAGGAAACACACCACAA CCAGAGCTACTCTGTGGCACCGGGAGGGGACCCGAGAGAGTAGAGCAAAGCAGTGCTTTTTGTTTTGTTT TGAGAGAAGATCTTGCTATGAAGACCAGCCTGGCCTCAAACTCCTGATCCTCCTGCCTCAGCCTCCTGAG AGCTGAGATGGCAGGTGTGTGACCCCCCAGGCTGGGCTGGTGGTTTGTAAGTTGTCTTTATCCAGCATCA GAAGATGAGAACCAAGATGGTGCCTGGGGCCAGGTCCACAGACAGCCCCTCTTGGCTTTTTGAAAACAGA ATCCTGGAGGCTTTTTCTGACTAGGCGGCTGCTGGTGACAGCCAAGGCCTATGAGGTGAGAGGATCTCTG TGTCTGGGATGGATGGATGGATGAATGGACACAACCCTGCTGTCCCCAAAGGTCTAAGGTCCTGCCTCCA AGCCCAGGAACTCCAGTGGGGGCCACCTGCTCCATCCACCTCCTTGCTGCCATGGCATGCTCACCGCCCC TTTGGAATCACCAGCACCCACTGATACAGGCAAGCAGAACCCACCAGGGCCACTGAAGACCCCCCAAACA GGAGACCCCGAGGGGCTTAATATTCTAGCCCACACCTGGGACCACCACCCCAGGTACCTGGGGGACAGTG AGGCAAGACACAGAGGTCCAGCAGCCTCATGGGGAAGGGCTGTTCCATTTCTTTACTGAGACACCCAGGA TCCATTTGGCAAGTGACCCAGAGCAACAGTGTCCACCGCCCACCAGCAAATTGTGCAGTTATAAACACAG AGACGGTGGGGACAGTCCCCAAAGTAACACAAAGTCCGGGGAGGCTGGGGTGGGGATTTTTATGTGAAAT AAATAAATTATAATGCAGAGGGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6491141 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6491141 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14352 same embryo
	EMAGE:14356 same embryo
	EMAGE:14353 same embryo
	EMAGE:14355 same embryo
	EMAGE:14354 same embryo
	EurExpress:euxassay_004136 same experiment
	MGI:4823288 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source