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EMAGE:15153

Ldlr low density lipoprotein receptor ( MGI:96765)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15153 EMAGE:15153 EMAGE:15153 EMAGE:15153 EMAGE:15153
"Pseudo-wholemount" of euxassay_010458. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010458_01 euxassay_010458_02 euxassay_010458_03 euxassay_010458_04
EMAGE:15153 EMAGE:15153 EMAGE:15153 EMAGE:15153 EMAGE:15153
euxassay_010458_05 euxassay_010458_06 euxassay_010458_07 euxassay_010458_08 euxassay_010458_09
EMAGE:15153 EMAGE:15153 EMAGE:15153 EMAGE:15153 EMAGE:15153
euxassay_010458_10 euxassay_010458_11 euxassay_010458_12 euxassay_010458_13 euxassay_010458_14
EMAGE:15153 EMAGE:15153 EMAGE:15153 EMAGE:15153 EMAGE:15153
euxassay_010458_15 euxassay_010458_16 euxassay_010458_17 euxassay_010458_18 euxassay_010458_19
EMAGE:15153
euxassay_010458_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15153Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15153_wholemount_strong.wlz
15153_wholemount_moderate.wlz
15153_wholemount_weak.wlz
15153_wholemount_possible.wlz
15153_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15153_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
weak weak
regionalweak expression: see section 01 02 08 12 14 15 16 17 18
adrenal gland
weak weak
regionalweak expression: see section 02 03 04 11 12 13
submandibular gland primordium
weak weak
regionalweak expression: see section 05 06 07 16
ventral grey horn
weak weak
regionalweak expression: see section 06 08 09 10
cervico-thoracic ganglion
weak weak
regionalweak expression: see section 05 06 12
cervical ganglion
weak weak
regionalweak expression: see section 05 06 13
thoracic ganglion
weak weak
regionalweak expression: see section 07 08
otic capsule
weak weak
regionalweak expression: see section 05 06 13 14
hindgut
weak weak
regionalweak expression: see section 08 09 10
midgut
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 10 11 12 13
mandible
moderate moderate
regionalmoderate expression: see section 01 02 weak expression: see section 03 05 06 07 08 10 15 16 17 18 19 20
lower jaw incisor
weak weak
regionalweak expression: see section 11 12 15
maxilla
weak weak
regionalweak expression: see section 05 06 07 08 19 20
upper jaw incisor
weak weak
regionalweak expression: see section 11 15
metanephros
weak weak
regionalweak expression: see section 02 03 04 11 12 13
orbito-sphenoid
weak weak
regionalweak expression: see section 01 02 03 04 05 06 18 19 20
viscerocranium
weak weak
regionalExpression in the turbinate bone.
clavicle
moderate moderate
regionalmoderate expression: see section 07 12 weak expression: see section 06 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30223
Entity Detected:Ldlr, low density lipoprotein receptor ( MGI:96765)
Sequence:sense strand is shown

>T30223
GGTTGCCCTCCTTGTCCTTGGGGCCGTCCTGCTGTGGAGGAACTGGCGGCTGAAGAACATCAACAGCATA
AACTTTGACAACCCAGTCTACCAGAAGACCACAGAGGATGAGCTCCACATTTGCCGAAGCCAGGACGGCT
ATACCTACCCCTCAAGACAGATGGTCAGCCTGGAGGACGATGTGGCATGAGCAGCCGGGAGAGCCGTCTC
TTTCCGGGATCCATTGCCAAGCTTAGGCAGAAAAGACACTCTCTCCAGACCTCCCCATCCAGCACTGGTC
CTGCCACCTCCCTGGGCTCTGTGTTGCTCAAAGCAAGATAAGAGCAAAGCTGGGCTGGGGGCCAAGCTCA
GCTGCCTGTCTGCCCCAGGTTCTGTTTTATATATTTATTGTCTGGGGACAGAAAAGGCTACTGGCTGTGC
TTGAAATTCGAATTCTGCCTGGGAATTTTTTTTTTCTCCCATCTTCATTTCCTTGGAAGCCAAAAAGGCT
GTCCCAGAAGCTTCTGAATTCTACTCTTTTCAACACTAACACGGAGGAGGAAGAGACCCACCCAGGCAGA
GAGAAGCCTGCTGGACATGAGACAGCAGGTACTACTGACCGGGTCCTGGGTACCATAGCCAGTCCCTAAC
CTCAGGACTTCCTGCATTGACCTTCATCCCAGAGCCTTCCTGCCTGACTCTGCCGCCCCCATCAGAAGCC
AGGAAAGTGACTCGTGACATTCGGTACTGATCTTGCTGGATCTTGAGGCCGGCAGCTGCCAGTGATGGAT
ACCCGCTCATGCCTTTGCCAAAACGTCACCCGTTCCTGGAGGGACAAGCCCAAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5052599), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 24147. Forward Primer - name:024147_F_IRAV55-58_F16_Ldlr, sequence:GGTTGCCCTCCTTGTCCT; Reverse Primer - name:024147_R_SP6_IRAV55-58_F16_Ldlr, sequence:ACTTGGGCTTGTCCCTCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15155 same embryo
 EMAGE:15151 same embryo
 EMAGE:15154 same embryo
 EMAGE:15152 same embryo
 EurExpress:euxassay_010458 same experiment
 MGI:4825892 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS