EMAGE mouse gene expression spatial database

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EMAGE:15311

Rnf180 ring finger protein 180 ( MGI:1919066)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010508. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010508_01	euxassay_010508_02	euxassay_010508_03	euxassay_010508_04

euxassay_010508_05	euxassay_010508_06	euxassay_010508_07	euxassay_010508_08	euxassay_010508_09

euxassay_010508_10	euxassay_010508_11	euxassay_010508_12	euxassay_010508_13	euxassay_010508_14

euxassay_010508_15	euxassay_010508_16	euxassay_010508_17	euxassay_010508_18	euxassay_010508_19

euxassay_010508_20	euxassay_010508_21	euxassay_010508_22	euxassay_010508_23	euxassay_010508_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15311_wholemount_strong.wlz

15311_wholemount_moderate.wlz

15311_wholemount_weak.wlz

15311_wholemount_possible.wlz

15311_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15311_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foot

moderate

regional

moderate expression: see section 10 11 12 23 24

adrenal medulla

moderate

regional

moderate expression: see section 07 08 09 15 16

vibrissa

strong

regional

strong expression: see section 07 08 09 21 22 23

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15

telencephalon ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15

pons ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 19 20 21 22

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 08 18 19

spinal cord ventricular layer

moderate

regional

moderate expression: see section 10 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 14 15 16 17

lens

strong

regional

strong expression: see section 02 03

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30548
Entity Detected:	Rnf180, ring finger protein 180 ( MGI:1919066)
Sequence:	sense strand is shown >T30548 ACCAAGCAGCACAGGGAGGGAGACTAATGAGACCAGCTCTGAAACATTTGCCACATCCCGGAGTCCCGTC AGGTTGTGATAAGGAAACTCTGCTGACAGGTGGTGGCTCCAAAACCAGAAACCACTGGCTTTTAAGCATG GCCCGAAACAGTAACGGCCTCGGAAGACTTACAGAAGCACTCTGCCTGGAGGTGCGAGCGACGTATTTTG AGATGAAGAATGAAAAACTGCTCTTCAAAGCCTCGGACCCAAAATGCCAGCCTTTTGTTCCTCAGCCTGA CACCGGCAGGTGTCCTTCAAGAGCGTCTCACAGAAAGTCACACAGTTTGGACCTGAACATCAGTGAGAAG CTGATTTTATTACCCACTTTATATGAAATACATCGTAAGCCTACTGCCTATCCCAGGCTAAATGAAACGG GGCCCATTGACCTTTCAGGCTTGGCTTTACCGTGTAGTAACAGTAGCTGCTCCTTTCAAAGCCCACCTAG TTTTGATCCGAATATGCTGCTGCACAGACTTTCAGTGGCTCCCCATGAGACCCAGGCACAAAGGGGAAGA GAATGCCAGTGCGGCCTAGAAGCTTCTTCAGTCTACTCGGATCACGCTAACGCTAACAGCCTGCCATTCC TGATGGATCTGCCCTCAGCAGGGAGGAGCGTGCTGGAGGCCTCGGACCAAGAGGAGCACCTCTCCCAGCT GGACTTCCTGCGCTCTGCCAGCTTCCCCTTGGGCACCATTAACCACAGGCTGAACAACAGGGAGAGAAGC AAGTTGAGGACTCTGCGAAGGCAGCAGAGGCGCGAACGATGGCTACAGAAGCAGGGTAAATACTCAGGAG TGGGCTTGCTGGATCACATGACCGTGAGTAATGAGATGAGTACAGATGAGGAAACGGAGTTCCCAGAGGA GAAGGACAGCTACATGTGTGCAGTGTGCCTGGATGTGTACTTCAACCCCTACATGTGCTACCCCTGCCAC CACATCTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5708557), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58946. Forward Primer - name:058946_F_IRAV111_e03_3110001E11Rik, sequence:ACCAAGCAGCACAGGGAG; Reverse Primer - name:058946_R_SP6_IRAV111_e03_3110001E11Rik, sequence:GAAGATGTGGTGGCAGGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15316 same embryo
	EMAGE:15314 same embryo
	EMAGE:15312 same embryo
	EMAGE:15315 same embryo
	EMAGE:15313 same embryo
	EurExpress:euxassay_010508 same experiment
	MGI:4827767 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source