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EMAGE:17065

Abcf3 ATP-binding cassette, sub-family F (GCN20), member 3 ( MGI:1351656)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17065 EMAGE:17065 EMAGE:17065 EMAGE:17065 EMAGE:17065
euxassay_001448_01 euxassay_001448_02 euxassay_001448_03 euxassay_001448_04 euxassay_001448_05
EMAGE:17065 EMAGE:17065 EMAGE:17065 EMAGE:17065 EMAGE:17065
euxassay_001448_06 euxassay_001448_07 euxassay_001448_08 euxassay_001448_09 euxassay_001448_10
EMAGE:17065 EMAGE:17065 EMAGE:17065 EMAGE:17065 EMAGE:17065
euxassay_001448_11 euxassay_001448_12 euxassay_001448_13 euxassay_001448_14 euxassay_001448_15
EMAGE:17065 EMAGE:17065 EMAGE:17065 EMAGE:17065 EMAGE:17065
euxassay_001448_16 euxassay_001448_17 euxassay_001448_18 euxassay_001448_19 euxassay_001448_20
EMAGE:17065
euxassay_001448_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17065Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17065_wholemount_strong.wlz
17065_wholemount_moderate.wlz
17065_wholemount_weak.wlz
17065_wholemount_possible.wlz
17065_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17065_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
limb
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 18 19 20 21
head mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 11 12 13 14 15 16 17 18 19 20 21
vertebral axis musculature
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
tongue mesenchyme
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16
tail
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1259
Entity Detected:Abcf3, ATP-binding cassette, sub-family F (GCN20), member 3 ( MGI:1351656)
Sequence:sense strand is shown

>T1259
TCGAGNCTGTTGGCCTACTGGGACTGCGCAGATTGGATCCCGAGTGAACATGGCGACTTACGCTGATATC
CTGCGAAGCGAGTTCCCTGAAATTGACGGGCAGGTCTTCGACTACGTGACCGGTGTCTTGCACAGTGGCA
GCGCGGATTTCGAGTCTGTGGATGACCTGGTGGAAGCTGTCGGGGAACTATTGCAAGAAGTGTCCGGGGA
CAGCAAGGATGACGCTGGCATTAGGGCTGTCAGTCAGCGCATGTACAACACTCTACGCCTGGCTGAACCG
CAGAACCAGGGAAACAGCCAGGTGCTACTGGACGCCCCCATCCAGTTGTCGAAGATAATGGAAAACTATG
ACTGTGACACCAAACTTCCAGGACTGCTAAAGAGGGAACAGTCCTCGACAGTAAATGCGAAGAAACTAGA
GAAGGCTGAAGCTCGACTGAAGGCAAAGCAGGAGAAGCGTTCAGAGAAGGAAACACTGAAGACCAGCAAC
CCTCTAGTCTTGGAGGAGGCGTCAGCTAGCCAGGCAGGCAGCAGAAAAGAGAGTCGATTGGAATCATCTG
GCAAGAACAAGTCCT
Notes:The probe template was PCR amplified from IMAGE:2225518 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2225518 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17064 same embryo
 EMAGE:17066 same embryo
 EMAGE:17063 same embryo
 EMAGE:17062 same embryo
 EurExpress:euxassay_001448 same experiment
 MGI:4822870 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS