EMAGE mouse gene expression spatial database

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EMAGE:17137

Nipal3 NIPA-like domain containing 3 ( MGI:1921802)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001516. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001516_01	euxassay_001516_02	euxassay_001516_03	euxassay_001516_04

euxassay_001516_05	euxassay_001516_06	euxassay_001516_07	euxassay_001516_08	euxassay_001516_09

euxassay_001516_10	euxassay_001516_11	euxassay_001516_12	euxassay_001516_13	euxassay_001516_14

euxassay_001516_15	euxassay_001516_16	euxassay_001516_17	euxassay_001516_18	euxassay_001516_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17137_wholemount_strong.wlz

17137_wholemount_moderate.wlz

17137_wholemount_weak.wlz

17137_wholemount_possible.wlz

17137_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17137_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

weak

regional

weak expression: see section 11 12 13 14

brain

weak

regional

weak expression: see section 01 02 03 04 05 06 07 09 10 11 13 14 15 16 17 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 05

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 15 16

vagus x ganglion

moderate

regional

moderate expression: see section 06 07 14 15

spinal cord

weak

regional

weak expression: see section 05 06 07 09 10 11 13

cervico-thoracic ganglion

weak

regional

weak expression: see section 07 13

cervical ganglion

weak

regional

weak expression: see section 07

thoracic ganglion

weak

regional

weak expression: see section 10

nasal cavity epithelium

weak

regional

weak expression: see section 15

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 11 12 14 15

esophagus

weak

regional

weak expression: see section 10 11

stomach

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09

hindgut

weak

regional

weak expression: see section 11 12 13 14 15

midgut

weak

regional

weak expression: see section 10 11 12 13 14 15

oral epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 15

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

bladder

moderate

regional

moderate expression: see section 12 13

kidney calyx

weak

regional

weak expression: see section 05 06 08

kidney pelvis

weak

regional

weak expression: see section 07

lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 11 12 14 15 weak expression: see section 13 16

nucleus pulposus

moderate

regional

moderate expression: see section 10 11

tail dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 13 14 15

rectum lumen

moderate

regional

moderate expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4530
Entity Detected:	Nipal3, NIPA-like domain containing 3 ( MGI:1921802)
Sequence:	sense strand is shown >T4530 GAAGGAACGTCCCGGAGCTTGGCCAGAGTCAGAAGACACACCCTCACCCATCTGCTTGGAGGAGAGACAC CGCTTCCTTCTCCGGGAAGGCTGTATGTAAATCAACCAAAAGAGATCAGAGGACTCCTTTGGCTGAGGAA GGGAGCGAGGCGGCTGCTGGAAGGAGGAGAACACCGGGAGACATGGCTTCCAGTTCACACCCTGCCTAGC AGCAGCTCCAGCGCCCAGCCTGGCCCGGCAGGATGTCCCGCTAGACAGCCATGGACGGAGCCCACAGTGC AGGTCTGCAGCTGCAGCCACTCCCACCCACCAGCGGCGCCACCTCCACGAGCCTGAGCAGCAGCGAGGGC TCCTTCTCCTACAAGGAAAACTTGATCGGAGCACTCTTGGCCATTTTTGGGCACCTGGTGGTCAGCATTG CCCCTAACCTGCAGAAATACTGCCACATCCGCCTAGCAGGCTCCAAGGACCCACGGGCCTACTTCAAGAC CAAGACATGGTGGCTGGGGCTGCTCCTGCTGCTACTGGGGGAACTGGGCGTGTTCGCCTCCTACGCCTTT GCTCCACTGTCGCTCATCGTGCCCCTCAGTGCCGTCTCCGTGATAGCAAGCGCCATCATCGGAATCATAT TCATCAAGGAAAAATGGAAACCTAAGGACTTTGTGAGACGCTATGTCTTGTCCTTTGTTGGCTGCGGCTT GGCCATCGTGGGCACCTACCTGCTAGTGACATTTGCACCCAACAGTCATGAGAAGATGACAGGCGAGAAC ATCGCCAGGCACCTTGTGAGCTGGCCGTTTCTCCTGTACATGCTGGTGGCGATCGTCCTGTTCTGCCTGC TGCTCTACTTCTACAAGGAGAGGAACGCCAACAGCATCGTTGTGATTCTCCTCCTGGTGGCCCTGCTTGG TTCCATGACAGTGGTGACGGTCAAGGCCGTGTCAGGGATGCTGGTGCTGTCCATCCAAGGCAACCTGCAG CTGGACTACCCCATCTTCTACGTGATGTTTGTGTGCATGGTGGCCACCGCCATCTATCAGGCCACGTTTC TGAGTGAAGCCTCCCAGATCTATGACTCCTCCTTGATTGCCAGCGTTGGCTACATCCTGTCCACAACAGC GGCCATCACAGCAGGTGCGATCTTCTACCTGGACTTCCTTGGGGAGGAAGCTCTGCACATCTGTATGTTT GCACTGGGGTGCCTCATCGCCTTCTTGGGCGTGTTCCTAATCACTCGAAACAGAAAGAAGGCCATTCCGT TCGAGCCCTATATTTCCATGGACGCCATGCCAGGTATGCAGGACATGCATGCCAAAGGGACAACGGTCCA GCCTGACCTCAAAGCTTCTTTTTCCTACGGAGCCTTGGAAAGCAACGACAGCATTTCTGAGCTCTACACC CCGGCCACACTGCCCGTCATGCAGGAGGAGCACAGCTCCAGGAGCACCCCTGGGGTTCCCTACCGCGTCC TGGAGCACACCAAGAAGGAATGACCGGCCTCCCTTTGGGGTGTCACTGCCCGGAGGTCACCCATGGTTCC GTTCTTCATTGCCCAAACTGCCTTTCAAGTATCCTGGTTTCAAACGGAGAACTCTGTGCAAGATGGCCTT GCAAGGCCCCTGTCTCTCGGGCAGAGAAGCCATTCGATGACCTTGGCTTTGGAAATTTCAAATAGTAGGG GGAGAATCCCAGGTGGGCTATGTGGCCGGTTATGCTAGTGGGAGTTGAAACCATTTTACCTCCTTGGACA GTCAGTGAGCGTCAGGTAACGGTCACAGGTTCCACACATTTCCTTGGAGTAAAAGCAAGACAGAGTGAAA AAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4973200 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4973200 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17139 same embryo
	EMAGE:17140 same embryo
	EMAGE:17141 same embryo
	EMAGE:17138 same embryo
	EurExpress:euxassay_001516 same experiment
	MGI:4826716 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source