EMAGE mouse gene expression spatial database

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EMAGE:17273

Asf1b ASF1 anti-silencing function 1 homolog B (S. cerevisiae) ( MGI:1914179)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001590. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001590_01	euxassay_001590_02	euxassay_001590_03	euxassay_001590_04

euxassay_001590_05	euxassay_001590_06	euxassay_001590_07	euxassay_001590_08	euxassay_001590_09

euxassay_001590_10	euxassay_001590_11	euxassay_001590_12	euxassay_001590_13	euxassay_001590_14

euxassay_001590_15	euxassay_001590_16	euxassay_001590_17	euxassay_001590_18	euxassay_001590_19

euxassay_001590_20	euxassay_001590_21	euxassay_001590_22	euxassay_001590_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17273_wholemount_strong.wlz

17273_wholemount_moderate.wlz

17273_wholemount_weak.wlz

17273_wholemount_possible.wlz

17273_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17273_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 08 moderate expression: see section 09 10 11 12

submandibular gland primordium

moderate

regional

moderate expression: see section 04 05 06 14 15 16 17

vibrissa

moderate

regional

moderate expression: see section 03 04 05 18 19

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 12 13

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 09 10 14 15 16

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 04 05 06 07 08 13 14 15 16 17 18 19

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 13 14 15 16 17 18 19

midbrain ventricular layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14

otic capsule

moderate

regional

moderate expression: see section 01 02 19 20 21 22

neural retina

moderate

regional

moderate expression: see section 01 02 21 22 23

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

basisphenoid bone

moderate

regional

moderate expression: see section 22

exoccipital bone

moderate

regional

moderate expression: see section 22 23

temporal bone

moderate

regional

moderate expression: see section 23

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 17 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5690
Entity Detected:	Asf1b, ASF1 anti-silencing function 1 homolog B (S. cerevisiae) ( MGI:1914179)
Sequence:	sense strand is shown >T5690 GCTGAGCGGAGGAGCGCTTCAGAGGGAAGAGTTGGGAGACGCAGGGAGGTTTCCGTCCTGTCGTCCCCCC CTTCTTCTCTGTCGCAAGTCACTGTGAAGAAGTCTCCACAGCAGCTGCGGCTCCCGGGCGCGGTGATGGC CAAGGTGTCGGTGCTGAATGTGGCTGTGCTGGAGAACCCGAGCCCTTTCCACAGCCCCTTCCGGTTCGAG ATCAGCTTCGAATGCAGTGAGGCCCTGTCTGACGACCTGGAGTGGAAGATCATTTATGTGGGCTCAGCTG AGAGTGAGGAGTTTGATCAGATCCTAGATTCAGTGCTGGTGGGCCCTGTCCCTGCAGGAAGGCATATGTT CGTCTTTCAGGCTGATGCCCCAAACCCATCCCTCATTCCTGAGACGGACGCCGTGGGTGTGACTGTGGTT CTCATCACCTGCACCTACCATGGACAAGAGTTCATCCGTGTGGGCTACTATGTCAACAATGAGTACCCAG ACCCAGAGCTTCGAGAGAACCCACCCCCAAAGCCAGACTTCTCTCAGCTACAGCGGAACATCTTGGCCTC TAACCCCCGGGTGACCCGATTCCATATCAACTGGGACAACAATCCAGACAGTCTGGAAGCCATAGAAAAC CAGGACCCCAATGTGGACTTCAGCCTTTCCCTCAGTTGCACTCCTGTTAAAAGCCTGGGCCTCCCTAGTT GCATCCCAGGCCTCCTCCCTGAGAACTCCATGGATTGCATCTGACAGGGGAGCCAGGTAGAGCTCAGTAG GACCACAGCAGTCTCCTTGCACACCTGGAGGGGACCTACAAGCCCTCAGTCAGACATGCTTGGTCATCTC AAGGATTTGGGGACCACCAGCTGTCTCCAGATCTGTCAGCCCCAAGAAAAAACAATGGGCCCCAATCTCT TCCATCCAGGTCCAGAAGAATCATCTCACTTATGACCTACTGACCTCTAAAAAGCAGGTACTTGTATTGC TCATTCTATGCGTCATTTTTCTTTATATATTATCAGTTTCTATGAGATGCTTTCTGAACACCACCTTCTG TGAGGCTTGCTTGGGCAGTGTCTGGCTAGCATTGGCCAGAGGTTATCACTCCTTCCAACTCTAGTTCTTG GGGACTAGGCAAGGAAGAAGTCCTAGTTCGAAGGCCAGCTCAATACAATGGCTATCCTTTCAGAGTGTCT AGGACATTCAGTCTAACTAGTACTACAAAACGCAAAAGTGAGCTCCCCAGGCCAATGAGGGATGTCAGGT ACTTTGGTCACAGCCACCTTTTCATCTCCTGACTGAGCCTGGCTTTCTGAGAGGATTGCCCTAGGTAGGT ATATTGGAGGTCCTGCTTCATGCATTCTGGCCCCATAGAGTCATCAGGGTTTGCTTTGACCACATGTTCA TTCTCATGCTATACCCAAATACTCTGGGATAAAGGAGGTGTTTGGCAGGCTGACCTGAAAAACTCCCATT CCATCCACCTCTATCCTAAGCCCTTGGTTCCCTTGCTGCTACATGTATGCTATTGTGATTGCAGTGTGTA TATTAAAAGGTTTTATATTAAAAGTTTTGGTTGTCTTAAGAATAAAAGCACTTCATCTAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2645378 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2645378 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17272 same embryo
	EMAGE:17271 same embryo
	EMAGE:17276 same embryo
	EMAGE:17274 same embryo
	EMAGE:17275 same embryo
	EurExpress:euxassay_001590 same experiment
	MGI:4823275 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source