EMAGE mouse gene expression spatial database

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EMAGE:17677

Fgfbp1 fibroblast growth factor binding protein 1 ( MGI:1096350)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006009. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006009_01	euxassay_006009_02	euxassay_006009_03	euxassay_006009_04

euxassay_006009_05	euxassay_006009_06	euxassay_006009_07	euxassay_006009_08	euxassay_006009_09

euxassay_006009_10	euxassay_006009_11	euxassay_006009_12	euxassay_006009_13	euxassay_006009_14

euxassay_006009_15	euxassay_006009_16	euxassay_006009_17	euxassay_006009_18	euxassay_006009_19

euxassay_006009_20	euxassay_006009_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17677_wholemount_strong.wlz

17677_wholemount_moderate.wlz

17677_wholemount_weak.wlz

17677_wholemount_possible.wlz

17677_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17677_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

single cell

strong expression: see section 11 12 13 14 15

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

diencephalon meninges

moderate

regional

moderate expression: see section 07 08 09 10 11 12 15 16 17

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 07 08 09 10 17

telencephalon meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21

hindbrain meninges

moderate

regional

moderate expression: see section 07 08 09 10 11 12 15 16 17

midbrain meninges

moderate

regional

moderate expression: see section 07 09 10 11 12 15 16 17

spinal cord meninges

moderate

regional

moderate expression: see section 08 09 10 11 12 15 16 17

naris

moderate

regional

moderate expression: see section 11 12 13 15 16

stomach

weak

regional

weak expression: see section 05 06 07 08 09 10 11

rectum

weak

regional

weak expression: see section 15

midgut

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16

oral epithelium

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

urethra of male

weak

regional

weak expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8382
Entity Detected:	Fgfbp1, fibroblast growth factor binding protein 1 ( MGI:1096350)
Sequence:	sense strand is shown >T8382 CTGGAAGCCTGCATTCCCCAGTACACCTGGATCTGCACACTCACAGAAAGGTGTCCACCCAGCTGACGTG ATGAGACTCCACAGCCTCATCCTGCTCTCCTTCCTTCTCCTGGCTACTCAGGCGTTCTCAGAAAAGGTCA GAAAGAGAGCCAAGAACGCACCACACAGCACAGCGGAGGAGGGGGTAGAGGGTTCAGCTCCCTCGTTAGG GAAGGCCCAGAATAAGCAGAGAAGCAGGACATCTAAATCTCTGACGCATGGCAAGTTTGTCACCAAAGAC CAAGCCACATGCAGATGGGCTGTGACTGAGGAGGAGCAGGGCATCAGCCTGAAGGTCCAGTGCACACAAG CCGATCAGGAGTTTTCTTGTGTTTTTGCTGGTGACCCAACTGACTGCCTTAAACACGACAAAGACCAGAT CTACTGGAAACAGGTTGCCCGCACGCTGCGCAAACAGAAAAATATCTGCAGGAACGCCAAGAGTGTCTTG AAGACCAGAGTGTGCAGAAAGAGATTTCCAGAGTCTAACCTCAAGCTGGTGAACCCCAACGCACGTGGAA ACACGAAGCCCAGGAAGGAGAAAGCAGAGGTCTCCGCAAGGGAGCACAACAAGGTCCAAGAAGCTGTCTC CACGGAGCCAAACAGGGTCAAAGAAGACATCACACTCAATCCAGCTGCGACCCAGACCATGGCCATTAGA GATCCAGAGTGTCTAGAGGATCCAGATGTGCTCAACCAGAGGAAGACCGCCCTGGAGTTCTGTGGGGAAT CTTGGAGCTCCATTTGCACATTCTTCCTCAACATGTTACAGGCGACATCATGCTAATGAAGTCAGAGGGA GCAGCCTGAGACATGTGGTATCAGAGCCCCACTGTCTGCTGGAAACTTCCCGAGTTCCCCACAAGATACA TTTCTAATATGGGAACAAAATGAAGTATTTAAACAGATTTTGTAAATTTTGTTTTTGTGTTTTAGAATTT GCTTTTTTTTTTCCACATCCTTGGATATGCTTTTCAGGGACGCTTTACCTCAGCGTATTCTTCCATGGAC CACAAACCTAGGTGCATATAAGTAGTGGATCAATGCATGTGATAATATGCAAGCAAGGAATTGTTGGCTG TATTTACCAGGATAATAAAGTTCTGGGCATTCTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3975150 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3975150 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17680 same embryo
	EMAGE:17676 same embryo
	EMAGE:17679 same embryo
	EMAGE:17678 same embryo
	EMAGE:17681 same embryo
	EurExpress:euxassay_006009 same experiment
	MGI:4824845 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source