EMAGE mouse gene expression spatial database

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EMAGE:19330

Tmem45b transmembrane protein 45b ( MGI:2384574)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002006. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002006_01	euxassay_002006_02	euxassay_002006_03	euxassay_002006_04

euxassay_002006_05	euxassay_002006_06	euxassay_002006_07	euxassay_002006_08	euxassay_002006_09

euxassay_002006_10	euxassay_002006_11	euxassay_002006_12	euxassay_002006_13	euxassay_002006_14

euxassay_002006_15	euxassay_002006_16	euxassay_002006_17	euxassay_002006_18	euxassay_002006_19

euxassay_002006_20	euxassay_002006_21	euxassay_002006_22	euxassay_002006_23	euxassay_002006_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19330_wholemount_strong.wlz

19330_wholemount_moderate.wlz

19330_wholemount_weak.wlz

19330_wholemount_possible.wlz

19330_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19330_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

strong

regional

strong expression: see section 08 09 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 03 17 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 04 05 15

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 16 17 18 19 20 moderate expression: see section 08

vagus x ganglion

moderate

regional

moderate expression: see section 14

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 04 05 06 15 16

spinal cord

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 06 12

cervical ganglion

moderate

regional

moderate expression: see section 06 13

thoracic ganglion

moderate

regional

moderate expression: see section 09 10 11

dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 07 11 12 13 14

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 13 14 16 17 18 19 moderate expression: see section 10 11 12

hindgut

moderate

regional

moderate expression: see section 10 11 12 13 14

midgut

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4599
Entity Detected:	Tmem45b, transmembrane protein 45b ( MGI:2384574)
Sequence:	sense strand is shown >T4599 TGCACCTCCTGGGCACCCGCGGTCACACGTGTGACCGAAGCTGGCAAGTGGACACCTCTGAGGCTCGGAA GAGGCATCCTGGAACAGCGATGGCAAACTTCAAGGGCCATGCTCTCCCAGGGAGCTTCTTTCTAATAGTG GGGCTGTGGTGGTCAGTGAAGTACCCACTGAAGTACTTTCACCACAAGGGCTTGAAGAATAACAGACTGA GCCGTCAGCAGGAGCGCATTGAGATCATTGAAGGTGCAGTGAAGACTCTGTTTGCAATCATTGGGATCCT GGCAGAGCAGTTTGTTCCAGATGGACCTCACCTGCACCTCTACCACGAGAACCAATGGGTCAAGCTGATG AATTGGCAGCACAGCACCATGTACCTATTCTTCGGTGTCTCGGGACTCATGGACATGATCACCTACCTTT ACTTCCACATCGTGCCCCTGGGGTTAGACAGAGTGGTTTTGGCTATGGCCGTATTCATTGAAGGTTTTCT CTTCTACTTCCACGTTCACAACCGCCCTCCCCTGGACCAGCACATCCACTCCCTGCTGCTGTTCGGTCTT TTCGGAGCAGCTGTCAGCATCTCTCTGGAGGTTATCCTTCGGGATAACATCGTGCTGGAGCTCTTCCGAA CCAGTCTCCTTATCTTGCAGGGAACCTGGTTCTGGCAGATTGGATTTGTCCTGTTCCCGCCATTTGGAAG GCCAGAATGGGACCAGAAGGACATGGACAACATCATGTTCATCACCATGTGCTTCTGCTGGCACTACTTG GTTGCTCTCTGCATCGTAGCCATCAACTACTCCCTTGTTTACTGCTTTCTGACTCGGGTGAAGAGACGTG CAGAAGGAGAAATCATTGGGATTCAGAAGCTGAAGTCTGATCACACTTACCAGTCAGCCCTTTTGAGTGG CTCGGATGAGGAGTGACATCAAGCATGGGGGTGTGGAGACCTCACATCCTTTTTAAACAACACTGGAAAG GATGCAGCCTGTCCCCTGTACTTCAAGGCTTGCCTTATGTGCTCCTGTCCCTGGTACTCCAAGGCTTGCC TTATGTGCTCCTGTCCCTGGTACTCCAAGGCTTGCCTTATGTGCTCCTGTCCCTGGTACTCCAAGGCTTG CCTTATGTGCTCCTAGGGCTCTATATGCTGCATCCCTCATCTACTACAAAGCTAGAGGCTCAAATACAGG AAATTAGACCTCAAGTGTTTTTTATGCACCATGTTATTGGAATATTTAAGCATAGTTTAACTTAAAATCT GTATTAGAGTAGTAACACTGTCCACACAGAAAAGTGAACACCTTCCAGAAGCAGGCCTGTCCTTATAGAG CAATGACTCGAAGACTCTGAATTACACTAGGAAGCGACCATGTGGATGAAGCAGGCCGGACATCACGTGC TGCTTCTGATAACTTGCCCCTCTTCTCTCAATCAAGCAGGTACTTTCTTCTCAGAAAAGTACCACAAGTT AGATGGCGACAAATGTACCACATTTTCTGTATCCATTCCTCTGTTGAGGGACATCTGGGTTCTTTCCAGC TTCTGGCTATTATAAATAAGACTGCTATGAACATAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4988382 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4988382 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19329 same embryo
	EMAGE:19328 same embryo
	EMAGE:19325 same embryo
	EMAGE:19327 same embryo
	EMAGE:19326 same embryo
	EurExpress:euxassay_002006 same experiment
	MGI:4828805 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source