EMAGE mouse gene expression spatial database

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EMAGE:19371

Loxl3 lysyl oxidase-like 3 ( MGI:1337004)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002198. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002198_01	euxassay_002198_02	euxassay_002198_03	euxassay_002198_04

euxassay_002198_05	euxassay_002198_06	euxassay_002198_07	euxassay_002198_08	euxassay_002198_09

euxassay_002198_10	euxassay_002198_11	euxassay_002198_12	euxassay_002198_13	euxassay_002198_14

euxassay_002198_15	euxassay_002198_16	euxassay_002198_17	euxassay_002198_18	euxassay_002198_19

euxassay_002198_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19371_wholemount_strong.wlz

19371_wholemount_moderate.wlz

19371_wholemount_weak.wlz

19371_wholemount_possible.wlz

19371_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19371_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

humerus

strong

regional

strong expression: see section 01 02 15 16

forelimb digit 1 metacarpal

strong

regional

strong expression: see section 04 17 18

forelimb digit 2 metacarpal

strong

regional

strong expression: see section 04 17 18

forelimb digit 3 metacarpal

strong

regional

strong expression: see section 04

hand mesenchyme

strong

regional

strong expression: see section 01 02 03 19 20

hindlimb digit 1 metatarsal

strong

regional

strong expression: see section 05 06 07

hindlimb digit 2 metatarsal

strong

regional

strong expression: see section 04 05 06 07 08

hindlimb digit 3 metatarsal

strong

regional

strong expression: see section 04 05 06 07 08

hindlimb digit 4 metatarsal

strong

regional

strong expression: see section 05 06 07 08

hindlimb digit 5 metatarsal

strong

regional

strong expression: see section 06 07 08

foot mesenchyme

strong

regional

strong expression: see section 01 02 03

otic capsule

strong

regional

strong expression: see section 01 02 03 04 05 06 12 13 14 15 16 17 18 19

lower jaw incisor

strong

regional

strong expression: see section 10 11 12

upper jaw incisor

strong

regional

strong expression: see section 12

axial skeleton

strong

regional

strong expression: see section 03

axial skeleton cervical region

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10

axial skeleton lumbar region

strong

regional

strong expression: see section 03 04 05 06 07 08 09

axial skeleton sacral region

strong

regional

strong expression: see section 06 07 08 09 10 11

axial skeleton thoracic region

strong

regional

strong expression: see section 03 05 06 07 08 09

basioccipital bone

strong

regional

strong expression: see section 14 16 18 19 20

basisphenoid bone

strong

regional

strong expression: see section 13 14 18 19 20

exoccipital bone

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

temporal bone

strong

regional

strong expression: see section 14 19 20

temporal bone

strong

regional

strong expression: see section 20

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 15 17 18

viscerocranium

strong

regional

Expression in the turbinate bone.

clavicle

strong

regional

strong expression: see section 06 10 11

axial skeleton tail region

strong

regional

strong expression: see section 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6037
Entity Detected:	Loxl3, lysyl oxidase-like 3 ( MGI:1337004)
Sequence:	sense strand is shown >T6037 GAAAGTAATCTCCAGGACCAGGACTCTGCTTCCAGCCTCTGGAGTTGTGCCTAGTCGAAGTGCCCCCCTC CTTGGGCCCCACCCGCCATGAGAGCTGTCAGTGTGTGGTATTGCTGCCCATGGGGACTACTGCTGCTACA CTGTCTGTGCAGCTTCAGTGTGGGGTCTCCGTCTCCTTCTATCAGCCCAGAGAAGAAGGTAGGAAGCCAG GGGCTGAGGTTCCGGCTGGCTGGCTTTCCTAGAAAGCCCTATGAAGGTCGAGTGGAGATACAGCGAGCTG GTGAATGGGGCACCATCTGTGATGATGACTTCACGCTGCAGGCTGCCCATGTCCTCTGCCGGGAGCTGGG CTTCACAGAAGCCACAGGGTGGACTCATAGTGCCAAATATGGCCCTGGAACAGGCCGAATCTGGTTGGAC AATCTGAGCTGCAGGGGGACTGAAGGAAGTGTGACAGAATGCGCCTCTCGGGGCTGGGGAAACAGTGACT GTACCCATGATGAGGATGCCGGGGTCATCTGCAAGGACCAGCGCCTTCCCGGTTTCTCAGACTCCAACGT CATTGAGGTGGAGCATCAGTTGCAAGTGGAGGAGGTGCGACTCCGACCTGCGGTGGAGTGGGGAAGGAGA CCCCTGCCTGTGACTGAGGGCCTGGTTGAAGTCAGGCTTCCTGAAGGCTGGTCGCAAGTGTGTGACAAAG GCTGGAGTGCCCACAACAGCCACGTAGTCTGCGGTATGCTGGGCTTCCCTGGAGAAAAGAGAGTCAACAT GGCCTTCTACAGGATGCTGGCCCAGAAGAAGCAACACTCCTTTGGTCTGCACAGTGTGGCATGTGTGGGC ACAGAAGCCCACCTCTCCCTTTGCTCTCTAGAGTTCTATCGTGCCAATGACACCACCAGGTGCTCTGGGG GAAACCCTGCAGTGGTGAGCTGTGTGCTGGGCCCTCTTTATGCCACCTTTACTGGTCAGAAGAAGCAGCA ACACTCTAAGCCTCAAGGGGAGGCTCGTGTGCGTCTAAAGGGTGGTGCCCACCAGGGAGAAGGCAGAGTG GAAGTCCTGAAGGCTGGCACATGGGGAACAGTCTGTGACCGAAAGTGGGACCTGCAGGCAGCCAGTGTGG TGTGTCGAGAACTGGGCTTTGGCACTGCCCGAGAGGCCCTGAGTGGTGCCCGCATGGGGCAGGGCATGGG TGCCATCCACTTGAGTGAAGTTCGGTGCTCTGGCCAGGAGCCCTCCCTTTGGAGATGCCCGTCCAAGAAC ATCACAGCTGAGGACTGTTCCCACAGCCAGGATGCGGGTGTCCGGTGCAACCTCCCCTACACTGGGGTGG AGACCAAGATCCGACTCAGTGGGGGCCGCAGCCGGTATGAAGGGCGAGTCGAGGTGCAAATAGGGATACC TGGGCATCTACGCTGGGGCCTCATCTGTGGGGATGACTGGGGAACCCTGGAGGCCATGGTGGCCTGTAGG CAACTTGGTCTTGGCTACGCCAACCACGGCCTGCAGGAGACCTGGTACTGGGACTCAGGGAATGTAACTG AGGTGGTGATGAGCGGAGTGCGTTGCACGGGGTCTGAGCTGTCCCTGAACCAGTGTGCCCATCACAGCAG CCACATCACCTGCAAGAAGACGGGAACCCGCTTCACTGCGGGGGTCATCTGTTCTGAAACTGCTTCAGAT CTGCTGCTGCACTCGGCACTGGTACAGGAAACTGCTTATATTGAAGACCGGCCCTTGCACATGTTGTACT GTGCTGCTGAAGAGAACTGCCTGGCTAGCTCTGCCCGCTCGGCCAACTGGCCTTATGGCCACCGGCGTCT GCTCCGATTCTCTTCCCAGATCCACAACCTAGGAAGAGCTGACTTTAGGCCCAAGGCTGGGCGCCATTCC TGGGTGTGGCATGAGTGTCATGGGCATTACCACAGTATGGACATCTTCACTCATTATGATATCCTGACCC CCAATGGCACCAAGGTGGCTGAGGGCCACAAAGCTAGTTTCTGTCTAGAAGACACTGAGTGTCAAGAGGA TGTCTCCAAGAGGTATGAGTGCGCCAACTTTGGAGAGCAGGGCATCACCGTGGGATGTTGGGATCTCTAC AGGCATGACATTGACTGTCAGTGGATCGACATCACAGATGTGAAGCCAGGAAACTACATTCTTCAGGTCG TTATCAACCCAAATTTTGAGGTAGCAGAAAGTGACTTCACCAACAATGCAATGAAGTGTAACTGCAAATA CGACGGGCATCGCATCTGGGTGCACAACTGCCACATCGGTGATGCCTTCAGCGAAGAAGCCAACAGGAGG TTTGAGCGCTACCCTGGCCAGACTAGTAACCAGATCGTCTAAAATGCTACCACCCTCTCTGTAAACCACC ACTGGCCCCCAATGGCAGGGGTCTGAGGCTGCCATTACCTCAGGAGCTTACCACAAGAACCCCTGATGTC AGCAGGCGCCCTACACTCAACCACCTGTGCACTACAGGCAACAAACTGTCAAACAGAAGTTGGTGCCTCC TTTACGGGGTCAGCTGTCATTACCTTTGGCCAACAAGCATGGAGAATCAGCCAACAGCCCCAGCAACAGA CAGAACACACCACAAAGAACTGCCCCACACAGGATGGCAGCAGCTCATTCTCTTGAATAGGGGAGGTCAA GAGGGTCAGCTCCCATCGCTCTCCGTTCTCCTAAGGACCGCGTTACCTCTAGCCATTTGGTGGGGGAGAA GATCCAACCCCCACTTTTGACTGTAACATGTTGCTAGGTATAATTTTATTTTATATAAAATGTGTTCTTC GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3488978 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3488978 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19376 same embryo
	EMAGE:19375 same embryo
	EMAGE:19373 same embryo
	EMAGE:19374 same embryo
	EMAGE:19372 same embryo
	EurExpress:euxassay_002198 same experiment
	MGI:4825963 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source