EMAGE mouse gene expression spatial database

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EMAGE:19404

Kpna1 karyopherin (importin) alpha 1 ( MGI:103560)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004798. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004798_01	euxassay_004798_02	euxassay_004798_03	euxassay_004798_04

euxassay_004798_05	euxassay_004798_06	euxassay_004798_07	euxassay_004798_08	euxassay_004798_09

euxassay_004798_10	euxassay_004798_11	euxassay_004798_12	euxassay_004798_13	euxassay_004798_14

euxassay_004798_15	euxassay_004798_16	euxassay_004798_17	euxassay_004798_18	euxassay_004798_19

euxassay_004798_20	euxassay_004798_21	euxassay_004798_22	euxassay_004798_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19404_wholemount_strong.wlz

19404_wholemount_moderate.wlz

19404_wholemount_weak.wlz

19404_wholemount_possible.wlz

19404_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19404_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pericardium

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 17 moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 18 19 20 21 22 23

adrenal gland

strong

regional

strong expression: see section 04 05 06 12 13 14 15

submandibular gland primordium

strong

regional

strong expression: see section 05 06 07 13 14 15 16

pancreas

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13

thyroid gland

moderate

regional

moderate expression: see section 11 12 13

adenohypophysis

moderate

regional

moderate expression: see section 08 09 10 11 12 13

physiological umbilical hernia epidermis

strong

regional

strong expression: see section 10

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

strong

regional

strong expression: see section 05 moderate expression: see section 03 04 06 17 18 weak expression: see section 19 20

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14

choroid invagination

weak

regional

weak expression: see section 06 07 08 13 14 15 16

telencephalon ventricular layer

moderate

regional

moderate expression: see section 10 weak expression: see section 02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 09

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 07 08 10 11 12 13 14 15 16

metencephalon rest of alar plate ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

pons ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 moderate expression: see section 05

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 15 16 17 18 19

spinal cord ventricular layer

strong

regional

strong expression: see section 09 10

inner ear

moderate

regional

moderate expression: see section 01 02 03 05 06 07 14 15 17 18 19 weak expression: see section 04 13 16

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 22 23

conjunctival sac

moderate

regional

moderate expression: see section 03 04 20 weak expression: see section 01 02

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 12 13 14 15 16

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 10 11 12 13 14

naso-lacrimal duct

strong

regional

strong expression: see section 02 03 04 05 06 07 15 moderate expression: see section 16 17 18 19

pharynx epithelium

strong

regional

strong expression: see section 08 09 10 11 12

stomach

moderate

regional

moderate expression: see section 01 02 03 07 08 weak expression: see section 04 05 06

rectum

strong

regional

strong expression: see section 09 moderate expression: see section 08

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

bladder

strong

regional

strong expression: see section 09 10 moderate expression: see section 11 12

metanephros

moderate

spotted

moderate expression: see section 04 05 06 07 12 13 14 15 16

urethra of male

strong

regional

strong expression: see section 08 09

lung

moderate

regional

moderate expression: see section 09 10 11 12 13 weak expression: see section 01 02 03 04 05 06 07 08 14 15 16 17 18 19 20 21

trachea

strong

regional

strong expression: see section 10 11 moderate expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6482
Entity Detected:	Kpna1, karyopherin (importin) alpha 1 ( MGI:103560)
Sequence:	sense strand is shown >T6482 GCGAGGCCTGGTGAGCGCCGCCGTGGTGCGCGCCGGCTGGTCAAGGTTGTGCGCAGAAGAGGCGCAGCGG GCGGGCCAAGAGCGAGCGTCTCCCTCTTCGTAGTGGCACCGGGACCGGTCGCCTGGAGCCGAAATCATGT CCACACCAGGAAAAGAGAACTTTCGCCTGAAAAGTTACAAGAACAAATCTCTGAATCCTGATGAGATGCG CAGGAGGAGGGAGGAAGAAGGACTGCAGTTACGGAAGCAGAAAAGAGAAGAGCAGTTATTCAAGAGAAGA AATGTTGCTACAGCAGAAGAAGAAACAGAAGAAGAAGTTATGTCTGATGGTGGCTTTCATGAGGCCCAGA TAAATAACATGGAGATGGCACCAGGGGGTGTCATCACCTCTGACATGACTGATATGATATTCTCGAATAG CCCAGAGCAGCAGCTCTCTGCAACACAGAAGTTTAGGAAACTACTTTCAAAAGAACCTAATCCTCCAATT GATGAAGTCATCAACACACCAGGAGTAGTGGCCAGGTTTGTGGAGTTCCTCAAACGAAAAGAAAATTGTA CACTGCAGTTTGAATCAGCTTGGGTCCTGACAAATATTGCTTCAGGAAACTCTCTTCAGACCCGGAATGT GATTCAGGCAGGAGCTGTGCCTATTTTCATCGAGTTGCTTAGCTCAGAGTTTGAAGATGTCCAGGAGCAG GCAGTTTGGGCTCTTGGCAACATTGCTGGAGATAGTACCATGTGCAGGGACTATGTCTTAAACTGCAATA TCCTTCCTCCTCTTCTGCAGTTATTTTCAAAGCAAAACCGCTTGACCATGACCCGGAATGCAGTATGGGC TTTATCTAATCTCTGTAGAGGGAAGAGCCCACCTCCAGAATTTGCTAAGGTTTCTCCCTGTCTGAATGTC CTTTCTTGGTTACTGTTTGTCAGTGACACTGATGTACTAGCTGATGCTTGCTGGGCTCTCTCATACCTGT CAGATGGACCCAATGATAAAATCCAAGCAGTCATCGATGCAGGAGTCTGCAGGAGGCTTGTGGAACTTCT GATGCATAATGACTATAAAGTAGTTTCTCCTGCTTTGCGGGCTGTGGGAAACATTGTCACAGGGGATGAT ATTCAGACACAGGTAATTTTGAATTGCTCAGCTCTTCAAAGTTTATTGCATCTGCTGAGTAGCCCAAAGG AATCTATCAAAAAAGAAGCCTGTTGGACAATCTCTAATATTACAGCTGGAAATAGGGCACAGATCCAGAC TGTGATAGATGCTAACATGTTCCCAGCTCTTATTAGTATATTGCAAACAGCAGAGTTCCGGACAAGGAAG GAAGCTGCTTGGGCCATCACAAATGCAACTTCTGGAGGATCAGCTGAGCAGATCAAGTACCTAGTAGAAC TGGGTTGTATCAAGCCACTCTGTGATCTCCTCACTGTCATGGATGCAAAAATTGTACAGGTTGCCCTAAA TGGCTTAGAAAACATCCTGAGGCTTGGAGAACAAGAAGCCAAACGAAACGGCTCAGGCATTAATCCTTAC TGTGCTTTGATTGAGGAAGCATATGGTCTGGATAAAATTGAGTTCTTACAGAGTCATGAAAACCAGGAGA TCTATCAAAAGGCTTTTGATCTTATTGAGCATTACTTTGGGACCGAAGATGAGGACAGCAGCATCGCACC CCAGGTTGATCTTAGCCAGCAGCAGTACATCTTCCAGCAGTGTGAAGCTCCTATGGAAGGTTTCCAGCTT TGAAGCAATCCTCTCCTTCTTGTTCCTGTGTCAGACCAGGCTATCCAGTCAAGTCCTCTTGTGGAGCCCA CAGTCCTCATGGAGCTAATCTCTCAAATGTTTTCCATAATACTGTTTGCGCTCATTTGCTTGCCTTGCGC ACCTGCTCTCTTACACACATCTGGAAAACCTCTGGCTCTCTGTGGTGGAATACCCTTTTATTACAAGGGT AACCAGAACGGCCCACTCTCTTATGGAAAAATCCCTAAGCTTTGGAGATCCGCACTTACATTAGAGTCAT GGGAATATATACACATACTAATGTGGCTCCCTTTTTTGTGGGGGAAAAAAGAGGACTCTTCCTCATTCCG TAAAGTGGGGAAAATGACATTGAAAGATAAAACTAAAACCTTTATCTTGAATTTTACACAACTACAAGGG AGATATTTAGACTTGTGTATACGTCAGAGTACTCTTCTCGAGTTCTTCCACAGTGAATCAACCCTTGGAG TACCTGGTGGCTTTTTCTAGCCACATTTGCATTAATCCTTATTGAGATGGGATGGTTTTCTTTCCTCTAA TGGAGTCATTTTCTTCAGATATTAAAGTTAAACCATCCAATCCCTCACCTTCAGCCTTCAGTGAATGTGC TTCTAGTTGTCAGGAATGCTGAAGAATTAACACTTTGACTTTTAAATGTGATCCTGGCTTGGCAGATTTC CTTAAAGCAGGAAGGAGAGAAGCATATATTAGTTTGTATGGCTTTTGCTTCTCTTTGTTTTTGTTTCTTA GGATTTGGAAGTGGTTCAGTTTTAACGCTGGTATGAAGGCTAAAAGTCCTTAAGTAAAACCATGTTCAAA AATCCAATTAAAGACTAAGAACATAAAACCCATCACTTTGTTTAAACCGGAAAAAAAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3588534 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3588534 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19402 same embryo
	EMAGE:19405 same embryo
	EMAGE:19403 same embryo
	EMAGE:19401 same embryo
	EMAGE:19406 same embryo
	EurExpress:euxassay_004798 same experiment
	MGI:4825819 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source