EMAGE mouse gene expression spatial database

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EMAGE:19408

6330527O06Rik RIKEN cDNA 6330527O06 gene ( MGI:1923411)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004790. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004790_01	euxassay_004790_02	euxassay_004790_03	euxassay_004790_04

euxassay_004790_05	euxassay_004790_06	euxassay_004790_07	euxassay_004790_08	euxassay_004790_09

euxassay_004790_10	euxassay_004790_11	euxassay_004790_12	euxassay_004790_13	euxassay_004790_14

euxassay_004790_15	euxassay_004790_16	euxassay_004790_17	euxassay_004790_18	euxassay_004790_19

euxassay_004790_20	euxassay_004790_21	euxassay_004790_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19408_wholemount_strong.wlz

19408_wholemount_moderate.wlz

19408_wholemount_weak.wlz

19408_wholemount_possible.wlz

19408_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19408_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

thymus primordium

strong

regional

strong expression: see section 07 08 09 10

thyroid gland

strong

regional

strong expression: see section 06 07 10 11

vibrissa

strong

regional

strong expression: see section 04 05 06 07 08 09 19 20 21 22

diencephalon lateral wall mantle layer

moderate

single cell

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 09 10 11

cerebral cortex mantle layer

strong

regional

strong expression: see section 14 15 16 17 moderate expression: see section 06 07 08

telencephalon mantle layer

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

medulla oblongata floor plate

strong

regional

strong expression: see section 06 08 09

medulla oblongata alar plate mantle layer

moderate

single cell

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14

medulla oblongata basal plate mantle layer

moderate

single cell

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14

metencephalon floor plate

strong

regional

strong expression: see section 06 07

rest of cerebellum mantle layer

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

pons mantle layer

moderate

single cell

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15

midbrain mantle layer

moderate

single cell

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14

spinal cord floor plate

strong

regional

strong expression: see section 06

spinal cord mantle layer

moderate

single cell

moderate expression: see section 04 05 06 07 08 09

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

vomeronasal organ epithelium

strong

regional

strong expression: see section 11 12 14

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07

rectum

strong

regional

strong expression: see section 09

midgut

strong

regional

strong expression: see section 09 10 11 12 13 14 15

lower jaw incisor

strong

regional

strong expression: see section 10 11 14

lower jaw molar

strong

regional

strong expression: see section 07 17

upper jaw incisor

strong

regional

strong expression: see section 10 11 12 15

upper jaw molar

strong

regional

strong expression: see section 07 17

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

bladder

strong

regional

strong expression: see section 09 10 11

trachea

strong

regional

strong expression: see section 08 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6487
Entity Detected:	6330527O06Rik, RIKEN cDNA 6330527O06 gene ( MGI:1923411)
Sequence:	sense strand is shown >T6487 CGCCTACACACTCAGAATGCTCTTTGTAAAGGAAAGTCACAACACTTCCAAAGGACCAGAGGCGACTTGG AATCTGAACAAGGTGCATTTTGTCTATGATTCCTCGGAGAAGACTCACTTTAAAGCCCCTGTGAAGGTTA ATAAGTACATCGCCAGCTCTCATCACCTCTCTGCCTTGGTCACCCCAGCTGGGATGTCCTATGAGTGTCA GGCTCAGCAGACCATTTCTCTGGCCTCCAGTGACCCTCAGAAGACTGTCACCATGATCCTGTCCGCAGTG CACATCCAGCCCTTTGATATCATCTCTGACTTTGTCTTCAGTGAAGAACATAAATGTCCAGTGGATGAGC AAGAGCAGTTGGAAGAGACCCTGCCCCTGATCCTGGGCCTCATCTTGGGCCTTGTCATTGTCATAACACT TGTGATCTACCACATCCACCATAAAATGACTGCCAATCAAGTGCAGATCCCCAGGGACCGTTCCCAGTAC AAGCACATGGGCTAGGGACCATCAGGCAGACAGCCTCCTCATCCTGCCCCCAAACCAGATGATGTAGAAC GACAATGGCACTTTTTCACCTGTATACAGGATCCACCAACATAGCTACACTCCAACAGGCCCAGGCACCT GAGGTGTGCCTGGCCTGTGTCCATGCTTAAACCCAGGGATGGAAGGCATGTCTTGAGGTTCGGGGGATTA ATGGTGGCTGTTGTGTCCATACTGCGGAGTAAGTAGGGGCTGGTGAGTAGGGTAATAGCTTTCTTATTCC TGATGGTTTAATTTTGATTCTCTGACTTTGTAAGTCTACAGCACGTAGAAAGAAAGAAAGATGTGGCCCT TGCATTTAGAAAGTGGAAACGCTTCCTTTGGGAGACCTCTCTAGGCCAGAAGAACCCAGGTGCTTCACTC CCTCGGCCTCAGCTGGCATACTTACACGGTTTTTCAGTGTACACAAAACACAACCTCACGCTCCCTGCAA GCAGCAAGACCCCTGAAAGTGACCCATGCTTCCGGCTGGCATTCTGCATGTCTAGTGATTGTTTTGGGAA TGTCTCACTGCTACCTGCACCCAGTGACTCTGCAGCACAAGAACCCGTCTAATGACACTACGCACGGTTG TTTGGAATTCATAATGTGTCGGTTCCAAGTGAGGGGACCTCCAGAATCAATCCATGTGAGTTCACTTTTG AAAATGAATTAAAAAGAAAAAAAAAAGCACTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3588577 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3588577 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19407 same embryo
	EMAGE:19411 same embryo
	EMAGE:19409 same embryo
	EMAGE:19410 same embryo
	EMAGE:19412 same embryo
	EurExpress:euxassay_004790 same experiment
	MGI:4822780 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source