EMAGE mouse gene expression spatial database

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EMAGE:19577

Rspo2 R-spondin 2 homolog (Xenopus laevis) ( MGI:1922667)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005010. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005010_01	euxassay_005010_02	euxassay_005010_03	euxassay_005010_04

euxassay_005010_05	euxassay_005010_06	euxassay_005010_07	euxassay_005010_08	euxassay_005010_09

euxassay_005010_10	euxassay_005010_11	euxassay_005010_12	euxassay_005010_13	euxassay_005010_14

euxassay_005010_15	euxassay_005010_16	euxassay_005010_17	euxassay_005010_18	euxassay_005010_19

euxassay_005010_20	euxassay_005010_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19577_wholemount_strong.wlz

19577_wholemount_moderate.wlz

19577_wholemount_weak.wlz

19577_wholemount_possible.wlz

19577_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19577_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 02 03 04 05 12 13 14 15 16 17 18 weak expression: see section 01

diencephalon floor plate

strong

regional

strong expression: see section 11

hypothalamus mantle layer

strong

regional

strong expression: see section 09 11 moderate expression: see section 08 12 13

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 14

diencephalon roof plate

strong

regional

strong expression: see section 10 11

lateral ventricle choroid plexus

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

pons mantle layer

moderate

regional

moderate expression: see section 04 06 13 14

midbrain floor plate

strong

regional

strong expression: see section 10 11

midbrain mantle layer

moderate

regional

moderate expression: see section 08 12 13 14

midbrain roof plate

strong

regional

strong expression: see section 10

extrinsic ocular muscle

strong

regional

strong expression: see section 04 16 17 18 moderate expression: see section 02 03 19 20

naris

strong

regional

strong expression: see section 06 13 14 moderate expression: see section 07

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 05 06 08 12 13 14 15 moderate expression: see section 07

vomeronasal organ

strong

regional

strong expression: see section 09 11 12

tongue muscle

moderate

regional

moderate expression: see section 08 09 10 11

stomach

moderate

regional

moderate expression: see section 01 02 03

lower lip

strong

regional

strong expression: see section 14 15 16 moderate expression: see section 04 05 06 13

mandible

strong

regional

strong expression: see section 03 16 17 18 moderate expression: see section 02 04 06 09 10

lung

moderate

regional

moderate expression: see section 03 06 07 08 09 10 11 12 13 14 15 16 17 weak expression: see section 04 05

axial skeleton

moderate

regional

moderate expression: see section 08 weak expression: see section 06 07 10

axial skeleton tail region

moderate

regional

moderate expression: see section 08 10 11 weak expression: see section 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8058
Entity Detected:	Rspo2, R-spondin 2 homolog (Xenopus laevis) ( MGI:1922667)
Sequence:	sense strand is shown >T8058 AAGAGGTAATCCTGCAGCTGTGCCATCTGCGTTAAACTGCTGCGTGGATCGTGCCAGTTCACCGTGGAGG GAGAGATGCTCATCGAGCCAAATTGATCATTGCAGCCGCAGGGCAGTGACATCTGTCTCTAAGTCCTCCC TAGGAGCGCGACCCGCACTGTCTCCTTCCAGGAGCCCGTCATTTCCTCGACTTTTGAGAGGTGTCTCTCC CCAGCCCGACCGTCCAGATGCGTTTTTGCCTCTTCTCATTTGCCCTCATCATTCTGAACTGTATGGATTA CAGCCAGTGCCAAGGCAACCGATGGAGACGCAATAAGCGAGCTAGTTATGTATCAAATCCCATTTGCAAG GGTTGTTTGTCTTGTTCGAAGGACAATGGTTGCAGCCGATGTCAACAGAAGTTGTTCTTTTTCCTTCGAA GAGAAGGAATGCGTCAGTATGGAGAGTGCCTGCATTCCTGCCCATCAGGGTATTATGGACACCGAGCCCC AGATATGAACAGATGTGCACGATGCAGAATAGAAAACTGTGATTCTTGCTTTAGCAAAGACTTTTGTACG AAGTGCAAAGTAGGCTTTTATTTGCATAGAGGCCGCTGCTTTGATGAATGTCCAGATGGTTTTGCACCGT TAGATGAGACTATGGAATGTGTAGAAGGTTGTGAAGTTGGTCATTGGAGCGAATGAGGAACGTGTAGCAG AAACAACCGCACGTGTGGATTTAAATGGGGTCTGGAAACCAGAACACGGCAGATTGTTAAAAAGCCAGCA AAAGACACAATACCATGTCCGACCATTGCGGAGTCCAGGAGATGCAAGATGGCCATGAGGCACTGTCCAG GAGGAAAGAGAACACCAAAGGCAAAAGAGAAGAGAAACAAGAAGAAGAGGCGGAAGCTGATTGAGAGAGC CCAAGAGCAGCACAGCGTCTTCCTCGCTACAGACAGAGTGAACCAATAAAATACAAGAAATAGCTGGGGC ATTTTGAGGTTTTCTGTTTTGTTTATGTTGTTGTTTTGCAAAAGTGCACAAAGCTACTCTCCAGTCCACA CTGGTGGACAGCATTCCTGATCCTCTGACCAGTATCCATTTTCAGTAATGCTGCAGAGGGAGGTGCCCAA GCATGGACTCAGCGTTATTTATGCTTTGATTGGAATCTGGGGCCTGTGATGGCAGGAGCTTGTTGAGCTG AGTCAGCGGGAGCTGATGCATCTGTACTCTTGTGATGAGCACAGTGTGTCATAAGAACCTGTCCCTGGCA CGGTGGACCCACAGGAGGCACAAGGCTGTAGATCACCACCAGAGAATGCACCTGTGCCTATTTTGATGGA TGGCAATGCTAAGCAAGCAAGCACTGTTCACTTGTGACTTTCATTTCTCACACTGTGCACTGTCAAAGAC AAATGTGCATGGAAAAATGTTTAGTGTCACCTCATGGCGTTCTCAGCATCAGTGACCTTCAAACGGTCCT ACAATGAGACTGTGTTCTAGCTAGGGGTATGCTGTGGAAATTCCTGCCACATTTCATCTTAGTGCTAACA TGTACAGATTCTGCTGCGCTACATTCAAAGCTCATTACTGTATATTTATGCTTTCTCTGTGTAACAAGTT ATACCTGATAAGATGTCACTTTGTTTCTAGTGGTTCTTAACCATGGTCTGGTACATGGCTATTCTAGTTT TGGAAATTAACAAGTGTTTTGTTGCCTCTTGTTTTCTTTTGTTCCTATCATTTTTGGCGGGGGTGGGATG GGCTTGATTCTAACCGTAAGTATAGGATAAGCTAGTTTTGTATATAGAGTCAAATGACTGATGTCAGAGG ATCAGTGCTGATAGAACTTCCCCAGTTCATGTCACCATACGCACAGAGAGAAAGCAGCATGAGGCATCTT GCCATCAGAAGCCAAATTTCTTTTGAGTCCCAAAATTGATGACTTATGAAATATAGCTGAAAACAAGATT TGGGTGTAGTTACTTGTATTTATTATACAATTTCCAATTACATTTTTTTTCAAACTCAAAATAACCCATG ACTTTGAGTGATAGGTCACTTGGCAATGTTCTTGAATTACTGGGGAAGCTGTTGTCACTAAGATAATGAG AGAGAAAATAGAATGGCTTCGCCCAAGTGAGAGCCACATCTTACATTTCTCTGTTGAATCGGAATCAACT ATATTAGAACAGAAGCCTGATGGAAGCTTTCTAGTTAACACACACAAGGCCATGGTTTCAAAAACATCTT TGTCCCCTTAGGTCAGTTTGTCCTTAGATTATGAATTGGCAGGTTCTAATTGCATTATTTCCCTGGCTGA TCCAGGAAAAAGTTAGAACAAAATAAGTTGCATAGTTTTGAGGAAACATCCAAAGCAAGGCGAAGCCTTT CCTTGCCTTGCATTGGCAAAACTACCTCTTTAGCATTTATGTTGATTCAGAAACATCTTGCTGATATGTG TAGATGTTTTAAGCTTCATTGTGAAAATATTGATGCAAGATAAGCCATATATGAATGTTGTATTCAACTT TAGGGCTTGAAATTAATCCTAAAGTGTTCACCTCTCTCCATGTCTATTTACACTCTGTTCCTATTTACTA AGAGGGTAGGGGTCTCCTTAATATCATACTTCATTGTTAATAAGTCAATGCTTGTTATGTTTCTTGGCTG TTGTTTTTGTGCTAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30054159 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30054159 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19576 same embryo
	EMAGE:19575 same embryo
	EMAGE:19574 same embryo
	EMAGE:19573 same embryo
	EMAGE:19578 same embryo
	EurExpress:euxassay_005010 same experiment
	MGI:4827826 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source