EMAGE mouse gene expression spatial database

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EMAGE:19642

Rilpl1 Rab interacting lysosomal protein-like 1 ( MGI:1922945)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005137. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005137_01	euxassay_005137_02	euxassay_005137_03	euxassay_005137_04

euxassay_005137_05	euxassay_005137_06	euxassay_005137_07	euxassay_005137_08	euxassay_005137_09

euxassay_005137_10	euxassay_005137_11	euxassay_005137_12	euxassay_005137_13	euxassay_005137_14

euxassay_005137_15	euxassay_005137_16	euxassay_005137_17	euxassay_005137_18	euxassay_005137_19

euxassay_005137_20	euxassay_005137_21	euxassay_005137_22	euxassay_005137_23	euxassay_005137_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19642_wholemount_strong.wlz

19642_wholemount_moderate.wlz

19642_wholemount_weak.wlz

19642_wholemount_possible.wlz

19642_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19642_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24 weak expression: see section 01 02 03 04 15

vertebral axis musculature

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24 weak expression: see section 01 02 03 04 15

adenohypophysis

moderate

regional

moderate expression: see section 11 12 13 weak expression: see section 10

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

not examined

regional

not examined expression: see section 03

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 14 15 16 17 18

medullary raphe

moderate

regional

moderate expression: see section 13

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 04

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 03

metencephalon rest of alar plate ventricular layer

moderate

regional

moderate expression: see section 19 20 21

pons ventricular layer

moderate

regional

moderate expression: see section 03 04 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

lens

strong

regional

strong expression: see section 01 02

metanephros

moderate

regional

moderate expression: see section 05 06 07 08 09 10 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8601
Entity Detected:	Rilpl1, Rab interacting lysosomal protein-like 1 ( MGI:1922945)
Sequence:	sense strand is shown >T8601 GGGACCGGAGCCGGGGGCGGAGCTTCGGCTTCGCGGGGCTGCGCACCGAGACCCCCGGGGACAGAGACTC GCTCCCCCTCCTCTCCAAACCCACGTCGCCTCGCAGCGTTCCCACGGGGAGGAGCCGGCCAACCGGAGTC ACTCGGCGTCACCAAAGTTTGATTCTCCTCTCCGGGAGGAGTCTCCGGGCCGCGGTCCCTCCGGGCACCG GGCGCCACCGCCTCCCAGGCCGGGACCCGCGTCTCCATCGCACCCAGTTGGAGCATCAAGTCCGGAGACG CTGCGGGCCTGCGCACGCGCCGCCGCCCTCGCCCTCCTCCCCGCGGTGCTCCAGGCCCCCCGCACTCCCC CCCCCCCAAGTTCACGAGTTACTGGAGTGGACGGACGGCTGGGAGCCCGCGCAGGGCCATGGAGGAGCCG CTAGGGTCACCGCCCGCCGCCCTGTCGGCTCTGGAGAAGAACGTGGCGGAGCTGACCGTCATGGATGTGT ACGATATCGCGTCGCTCGTGGGCCACGAGTTCGAGAGGGTCATCGACCAACACGGCTGCGAGTCCATCGC ACGCCTCATGCCCAAGGTCGTGAGGGTCCTGGAGATCCTCGAGGTGCTGGTTAGCCGGCACCACGTCGCT CCCGAGCTAGACGAGCTGCGCCTGGAACTGGACCGGTTGCGCGTAGAGAGGATGGACCGCATTGAAAAGG AGCGCAAGCATCAAAAGGAGCTGGAGCTGGTGGAGGACGTGTGGCGGGGTGAAGCACAGGACCTCCTGTC CCAGATTGCCCAGCTGCAGGAGGAGAATAAGCAGCTGATGACCAACCTCAACCACAAGGACGTGGGCTTC TCCGAGGAGGAGTTCCAGAAGCAGGAAGGCATGTCAGAGCGTGAGCGGCAGGTGATGAAGAGGCTGAAGG AGGTGGTGGACAAGCAGAGGGATGAGCTCCGAGCCAAAGACAGGGAGCTGGGCCTGAAGAATGAGGACGT CGAGGCTTTGCAGCAGCAGCAGACGAGGCTAATGAAGATCAACCATGACCTTCGGCACCGGGTCACCGTG GTTGAGGCCCAGGGGAAAGCCCTGATTGAGCAGAAGGTGGAGCTCGAAGCAGACCTGCAGACCAAGGAGC AGGAGATGGGCAGCCTGCGGGCTGAACTCGGAAAGCTTCGCGAGAGGCTGCAGGGCGAGCACAGCCAGAA TGGGGAGGAGGAGGAGGCTGAGATCCAGCCGCAGCCAGATGGAGAGGAATCGATTTCAGATGCAGAGAAG GCCGCCCTGGACCTCAAGGACCCCAACCGCCCGAGGTTCACGCTCCAGGAGCTGCGGGACGTCCTGCACG AGAGGAACGAGCTCAAGTCCAAGGTGTTTCTGCTGCAAGAGGAACTGGCCTACTATAAGAGTGAAGAAAT AGAAGAAGAGAATCGAATACCCCAGCCTCCGCCCATTACACACCCGAGGACGTCCCCCCAGCCTGAATCG GGGATCAAGCGACTGTTTAGCTTTTTCTCCCGGGACAAGAAGCGCCTGGCCAACACGCAGAGACCCACAC ACATCCACGAGTCCTTTGGCCAGTGGGCAATTACCCAGCGAGATGACGGGTACACAGAGCAAGGACAGGA AGCCCTGCAGCACCTGTGACTGTACCTCTGCCTGGACTGCCTGCCACCAGCTCTAGGTGGCGGCCGCCTC AACATCAGTTCCTCAAACGAACTGTCTGCTTTGAGGACTGATGACGAAAAACTGATGCCAAATTTGAAAG AAGTGTTTTATTTATCTGCAGGGCTACCCAGTTTATACTCAATGCCTCGAAACCCTTAGGACCTCTATTT AATAAGATGAGGATATGAAACCAGACCACATTAATTTCAGTTAACAGGCTTTCTAAGCTGAACACTTCGC CACTATCATAGCTGTTTGACTCCTAGGTTTTGTTTGTTTTCTTACGGCCTTTGTCGGTGAGGAGCAGAGG AAGGGAGTTTTATATCATGAAGTTGCCCTGGAGGTAGCAGGCTATACATAACAGGAAGCGTGAATCGGAC AGGTGGAGCCTCGGATCATAGGATGGACTACCCAGTTTGGCTGGGACACAACAGAAAATCATGCAGTTCT AGCCACTCGCTAGAAACAACCACAGGACTGTCACACTTTGTTCTGATAAAGCTAGAGGCCCAAGAAGCCT GTCCGAGATGGTGTGCCTTGGAATCTGCTGTGAGCTACGCAGTACTAACCAAACAATGTTGTTTAGGATG AGACATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5716540 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5716540 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19644 same embryo
	EMAGE:19647 same embryo
	EMAGE:19645 same embryo
	EMAGE:19643 same embryo
	EMAGE:19646 same embryo
	EurExpress:euxassay_005137 same experiment
	MGI:4827743 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source