EMAGE mouse gene expression spatial database

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EMAGE:19652

Rxrg retinoid X receptor gamma ( MGI:98216)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005164. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005164_01	euxassay_005164_02	euxassay_005164_03	euxassay_005164_04

euxassay_005164_05	euxassay_005164_06	euxassay_005164_07	euxassay_005164_08	euxassay_005164_09

euxassay_005164_10	euxassay_005164_11	euxassay_005164_12	euxassay_005164_13	euxassay_005164_14

euxassay_005164_15	euxassay_005164_16	euxassay_005164_17	euxassay_005164_18	euxassay_005164_19

euxassay_005164_20	euxassay_005164_21	euxassay_005164_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19652_wholemount_strong.wlz

19652_wholemount_moderate.wlz

19652_wholemount_weak.wlz

19652_wholemount_possible.wlz

19652_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19652_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 17 18 19 20 21 22 weak expression: see section 14 15 16

limb

strong

regional

strong expression: see section 01 02 03 04 05 20 21 22

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

hypothalamus

strong

regional

strong expression: see section 08 09 14 15 moderate expression: see section 13

diencephalon lateral wall

strong

regional

strong expression: see section 13 weak expression: see section 12

telencephalon mantle layer

strong

regional

strong expression: see section 03 04 05 17 18 19 20 moderate expression: see section 02 06

hindbrain

moderate

regional

moderate expression: see section 10

4th ventricle

strong

regional

strong expression: see section 02 03 04 05 06 12 13 14 15 16 17 18 19 moderate expression: see section 07 08 09

midbrain lateral wall

strong

regional

strong expression: see section 10 11 12 moderate expression: see section 04 05 weak expression: see section 08 09 13

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 17 weak expression: see section 15 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 17 18 19 20 weak expression: see section 07 15 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 04 05 06 07 14 15 16 17 moderate expression: see section 03 18

retina

strong

regional

strong expression: see section 01 02 21 22

tongue

strong

regional

strong expression: see section 09 10 11 12 13

tail

strong

regional

strong expression: see section 08 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8534
Entity Detected:	Rxrg, retinoid X receptor gamma ( MGI:98216)
Sequence:	sense strand is shown >T8534 GTTGGAGCGGCCGCGGCAGCAGTAGCAACAGCAGCAGCGACGGCGGCGGCAAGAGGCAAGGAGAGGTAGC AGCGGCGGCAGCATTATGCGTGATTACTGACAGGCACCAGCTGCTGCGGCCGCCACAGCCTCTCAACACT ATGTGGACTCTCGGATCTAGAGGCAGATTCCTGACTAATCCCAGAGGGCTGGCCCAGCCTGCGCTCTCCC GGCTGCTAGCAAGCGATGGCCACTCTTGTTAGCCCAAGTTGACGGAAGCCCGGCTGTGCCTGGGAGCCGA GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGCTGTACTCTTCAGAAGCGCAGCAG AGGAATGAACTGAGCAGCCAACATGTATGGAAATTATTCCCACTTCATGAAGTTTCCCACCGGCTTTGGT GGCTCCCCTGGTCACACTGGCTCGACGTCCATGAGCCCTTCAGTAGCCTTGCCCACGGGGAAGCCAATGG ACAGCCACCCCAGCTACACAGACACCCCAGTGAGTGCCCCTCGGACGCTGAGTGCTGTGGGAACCCCCCT CAATGCTCTTGGCTCTCCGTATAGAGTCATCACTTCTGCCATGGGTCCACCCTCAGGAGCACTGGCAGCT CCTCCAGGAATCAACTTGGTGGCTCCACCCAGCTCCCAGCTAAATGTGGTCAACAGTGTCAGCAGCTCTG AGGACATCAAGCCCTTACCAGGTCTGCCTGGGATTGGAAATATGAACTACCCATCCACCAGCCCTGGGTC TCTGGTGAAACACATCTGTGCCATCTGTGGGGACAGATCCTCAGGGAAGCACTACGGTGTGTACAGCTGT GAAGGTTGCAAAGGCTTCTTCAAAAGGACCATCAGGAAAGATCTCATCTACACCTGTCGGGATAACAAAG ATTGTCTCATCGACAAGCGCCAGCGCAACCGCTGCCAGTACTGTCGCTACCAGAAGTGCCTGGTCATGGG CATGAAGCGGGAAGCTGTGCAAGAAGAAAGGCAGAGGAGCCGAGAGCGAGCAGAGAGTGAGGCAGAATGT GCCAGTAGTAGCCACGAAGACATGCCCGTGGAGAGGATTCTAGAAGCCGAACTTGCTGTGGAACCAAAGA CAGAATCCTACGGTGACATGAACGTGGAGAACTCAACAAATGACCCTGTTACCAACATATGCCATGCTGC AGATAAGCAACTTTTCACCCTCGTTGAGTGGGCCAAACGCATCCCCCACTTCTCAGATCTCACCTTGGAG GACCAGGTCATTCTACTCCGGGCAGGGTGGAATGAACTGCTCATTGCCTCCTTCTCCCACCGCTCGGTTT CCGTCCAGGATGGCATCCTGCTGGCCACGGGCCTCCACGTGCACAGGAGCAGCGCTCACAGCGCGGGAGT CGGCTCCATCTTCGACAGAGTCCTTACAGAGTTGGTGTCCAAGATGAAAGACATGCAGATGGATAAGTCA GAGCTGGGGTGCCTACGGGCCATCGTGCTGTTTAACCCAGATGCCAAGGGTTTATCCAACCCCTCTGAGG TGGAGACTCTTCGAGAGAAGGTTTATGCCACCCTGGAGGCCTATACCAAGCAGAAGTATCCGGAACAGCC AGGCAGGTTTGCCAAGCTTCTGCTGCGTCTCCCTGCTCTGCGCTCCATCGGCTTGAAATGCCTGGAACAC CTCTTCTTCTTCAAGCTCATTGGAGACACTCCCATCGACAGCTTCCTCATGGAGATGTTGGAGACCCCAC TGCAGATCACCTGAACCTCCTCAGCTGCAGCTTCCCCACCCAGGGTGACCCTGGGCGGGTGTGTGTGTGT GGCCCTACCCTGCACACTCTCCCCCATCTTCCACTCTGGCCTCCCTTCCTGTCCCCAAAATGTGATGCTT GTAATAAAGACAACCTTTCTACACATGAGACTTTTCTAGGTGGAGTTTTGTACGGCTGTTAAAGGTGGCC CTTCTTTGCTATTTAAGGGGCTGAGGTCTTTCCGGCAGTTCTTGGGAAGAGTGGCCAAGCCTCTGTACAT ATAATTGGTTTAAATTATTTTTTCACTTGCCATGGAAAGCAAACAAATGGAAAAGAAAATAATAAATATG ATATTGGCACTGCTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5707723 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5707723 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19651 same embryo
	EMAGE:19653 same embryo
	EMAGE:19649 same embryo
	EMAGE:19648 same embryo
	EMAGE:19650 same embryo
	EurExpress:euxassay_005164 same experiment
	MGI:4827843 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source