EMAGE mouse gene expression spatial database

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EMAGE:19675

Tox3 TOX high mobility group box family member 3 ( MGI:3039593)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006138. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006138_01	euxassay_006138_02	euxassay_006138_03	euxassay_006138_04

euxassay_006138_05	euxassay_006138_06	euxassay_006138_07	euxassay_006138_08	euxassay_006138_09

euxassay_006138_10	euxassay_006138_11	euxassay_006138_12	euxassay_006138_13	euxassay_006138_14

euxassay_006138_15	euxassay_006138_16	euxassay_006138_17	euxassay_006138_18	euxassay_006138_19

euxassay_006138_20	euxassay_006138_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19675_wholemount_strong.wlz

19675_wholemount_moderate.wlz

19675_wholemount_weak.wlz

19675_wholemount_possible.wlz

19675_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19675_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand mesenchyme

moderate

regional

moderate expression: see section 01 02 20 21

foot mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 19 20 21

lower leg mesenchyme

moderate

regional

moderate expression: see section 19 20 21

pancreas

moderate

regional

moderate expression: see section 05 06 07 09 11 12

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 09 10 11 12

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 19 20

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 21

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 06 07 08 09 10 12 13 14 15 16 17 18 19 20

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 12 13 14 15 moderate expression: see section 08 09 10 11

metencephalon rest of alar plate mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

metencephalon rest of alar plate ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 10 12 13 14 15 16 17 18 moderate expression: see section 08 09 11

pons mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

pons ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 10 12 13 14 15 16 17 18 moderate expression: see section 09 11

midbrain mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16

midbrain ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14

facial vii ganglion

moderate

regional

moderate expression: see section 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 weak expression: see section 14

trigeminal v ganglion

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 14 15 16 17 18 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 02 04 05 06 13 16 17 weak expression: see section 03 14 15

spinal cord mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12

spinal cord ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 10 12 weak expression: see section 11 13

not examined

regional

not examined expression: see section 01 19 20 21

retina

moderate

regional

moderate expression: see section 01 02 19 20 21

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 03 04 06 07 09 10 12 13 14 weak expression: see section 08 11 15 16 17 18

pharynx epithelium

moderate

regional

moderate expression: see section 08 09 10

tongue

moderate

regional

moderate expression: see section 08 09 10 11 12

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07

midgut

moderate

regional

moderate expression: see section 09 12 weak expression: see section 10 11

renal cortex

moderate

regional

moderate expression: see section 02 03 04 05 06 11 12 13 14

genital tubercle of male

moderate

regional

moderate expression: see section 09 10 12 13 14

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8548
Entity Detected:	Tox3, TOX high mobility group box family member 3 ( MGI:3039593)
Sequence:	sense strand is shown >T8548 AGCCCCGGCCCCCAGCCCCCGGCCCCGGCATGGATGTGAGGTTCTACCCCGCGGCGGCCGGGGACCCAGC CGGCCTGGACTTCGCGCAGTGCCTGGGGTACTACGGCTACAGCAAGTTGGGAAATAATAACTACATGAAC ATGGCCGAGGCAAACAACGCCTTCTTTGCTGCCAGTGAGCAGACATTCCACACGCCAAGCCTTGGGGACG AAGAGTTTGAAATTCCACCGATCACGCCTCCTCCAGAGTCAGACCCCACCCTGGGTATGCCTGATGCACT GCTACCCTTTCAGACTCTCAGCGATCCGTTGCCTTCCCAGGGAACTGAGTTCACACCCCAGTTTCCCCCT CAAAGCCTGGATCTCCCTTCCATCACAATCTCAAGGAATCTGGTGGAGCAAGATGGCGTGCTTCATAGCA ATGGGCTGCACATGGATCAGAGCCACACACAAGTGTCACAGTACCGCCAGGATCCCTCTTTGGTCATGAG GTCAATTGTCCATATGACGGATGGTGCTCGATCTGGGATCATGCCTCCTGCCCAGCTGACCACCATCAAC CAGTCTCAGCTCAGCGCACAGCTGGGCTTGAATTTGGGAGGAGCCAATGTGTCCCACACATCTCCTTCAC CTCCAGCAAGCAAATCAGCCACTCCCTCTCCTTCCAGCTCTATCAACGAAGAGGATGCTGATGATGCAAA CAGAGCCATTGGAGAGAAAAGAACAGCCCCAGATTCTGGCAAGAAGCCCAAGACTCCAAAGAAAAAGAAA AAGAAAGATCCCAACGAGCCTCAGAAGCCAGTGTCAGCATATGCCCTGTTTTTCAGAGATACACAGGCTG CAATTAAGGGTCAAAACCCCAACGCAACCTTCGGAGAAGTCTCGAAAATCGTAGCATCTATGTGGGACAG CCTTGGGGAGGAGCAAAAGCAGGTATATAAAAGGAAAACAGAAGCTGCCAAGAAAGAATACTTGAAGGCC CTGGCTGCCTACCGGGCTAGCCTCGTTTCCAAGGCTGCTGCTGAATCTGCAGAAGCCCAGACCATCCGTT CTGTCCAGCAGACTCTGGCATCAACCAACCTGACATCCTCCCTCCTCCTGAACACATCACTGTCTCAACA TGGGACAGTCCCAGCCTCACCTCAGACTCTTCCACAGTCACTCCCTAGGTCAATTGCCCCCAAACCCTTA ACCATGAGACTACCCATGAGCCAGATCGTCACATCAGTCACCATTGCAGCCAACATGCCCTCGAACATTG GGGCTCCACTGATAAGTTCCATGGGGACGACCATGGTTGGTTCAGCAACCTCCACCCAGGTGAGCCCTTC GGTGCAAACCCAGCAGCATCAGATGCAGTTGCAGCAGCAACAGCAGCAGCAGCAGCAGATGCAGCAGATG CAGCAGCAGCAGTTACAGCAGCACCAAATGCATCAGCAGATCCAGCAGCAGATGCAGCAGCAGCATTTTC AGCATCACATGCAGCAGCACCTGCAGCAGCAGCAACAGCAGCATCTCCAGCAGCAGCTCAGCCAACAGCA GCTGCAGCAGCAGTTGCAGCAGCATCTCCAGCTGCAGCAACTGCAGCACATGCAGCACCAGTCTCAGCCT TCTCCCCGGCAGCACTCGCCCGTCACCTCACAGATCACGTCCCCCATCCCCGCCATTGGCAGCCCCCAGC CAGCCTCTCAGCAGCACCAGCCTCAAATCCAGTCGCAGACTCAGACTCAAGTGTTACCGCAGGTCAGTAT TTTCTAAGGTGCGCGAGGCACACGGATCTGAGTGAGGCGTAGGAGGGAAGGCGCATGGACTGGAACTTGC AAGTTGACTCTGGTTATGCAAGAATGTGTAACAGATCAAGAGATCCTCTCAAGTGTCTATTAGATAGGCA ATAAGAACTGCAGTGTAGCTGGGAAGCATCTTTTAGCCGACTATACATCACTTTGTTTTGAAACAAGGAA GCTGCGCTCTGTCATGTGCTGCCTTTTTTATTTATTCAGGCTAAACCTACAATATGTGAATCAACCCGTT TAATGAATCCTGGGACATGCTGATGACTGTAAACTGGCCTTTCCGAGTCATAGAAAAAAAAAAGATGGCC TTATTTCTCCAGAAGTGAGTGAACCACACTTCCAGGCTATTTGAACTTCTGAAGCCCTAAAATAAATAAC TAAGTACAAAGCACAGTTGTAACTACCTGAAATATGAAGATCCAGTTTCATACAAACATTTGTATGATGT GAATAGTTGATGGCATTTTTTTGTCATGAAAAAAAAATAATGTAAATCACAGACTTTTGCCAAAGCTCTT ATTTTTTTTCTAAATCTCTCCAGAAAAAAATTGCAAGTGATTAGATTCAATTATTGATTTATTTACTTCT ACTTTGTATCCATGACTTCTCCGAATCAAACCACGGTATATGTTGTGACAATATCTATGGCCACTGTTAG CCCATTATGTTCATTCCAATTAGAAGAAATGTGAATACTATATTACGTGTTTTGAGTGACGAGTTTGGGA AAATAAAAACACTGTATTTTTAAAAGGGAAATGCACTTAAATGAAAACAGTTATTACAAAAGTTAAGATT TAAAAAAAAGAAAAAGCAAGAGTTTTTATTATGATGTAATACCGGTAGAATATTGAATATTTAAAAGGCA CACTACATCTGATAGTCAATGTAAATATTTTCTTTCCAAGTTGTAAGTTTTCATATCATGTGTTGTAAAG TTTTCCTAAACGAGGCTTTAACGTAAACACTGGTGACATAAACCATTCATTGCTACCTTGCTTATTGTGT TTTTACGCGGTTTTATACTTTTTTTATGAGTTATGATAGCAGCAATTACGTTGTTTGTATTTTGCTTAAC TAAAACAAAAATGCTTTTATCTTGCTATAGAATAAACACATTTCAGTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5708447 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5708447 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19672 same embryo
	EMAGE:19674 same embryo
	EMAGE:19676 same embryo
	EMAGE:19673 same embryo
	EurExpress:euxassay_006138 same experiment
	MGI:4828872 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source