EMAGE mouse gene expression spatial database

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EMAGE:19767

Rassf4 Ras association (RalGDS/AF-6) domain family member 4 ( MGI:2386853)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006256. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006256_01	euxassay_006256_02	euxassay_006256_03	euxassay_006256_04

euxassay_006256_05	euxassay_006256_06	euxassay_006256_07	euxassay_006256_08	euxassay_006256_09

euxassay_006256_10	euxassay_006256_11	euxassay_006256_12	euxassay_006256_13	euxassay_006256_14

euxassay_006256_15	euxassay_006256_16	euxassay_006256_17	euxassay_006256_18	euxassay_006256_19

euxassay_006256_20	euxassay_006256_21	euxassay_006256_22	euxassay_006256_23	euxassay_006256_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19767_wholemount_strong.wlz

19767_wholemount_moderate.wlz

19767_wholemount_weak.wlz

19767_wholemount_possible.wlz

19767_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19767_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 05 06 07 moderate expression: see section 12 13 14 15

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 07 moderate expression: see section 08 09 10 11 12 13

rest of cerebellum ventricular layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 13 14 15 16 17 18 19 moderate expression: see section 08 09 10 11 12

pons ventricular layer

strong

homogeneous

strong expression: see section 05 06 07 16 moderate expression: see section 08 09 10 11

midbrain ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 16 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

ventral grey horn

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 11 12 13 14 15

neural retina

strong

regional

strong expression: see section 01 02 03 04 22 23 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 14 15 16 17 18 19

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 13 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8812
Entity Detected:	Rassf4, Ras association (RalGDS/AF-6) domain family member 4 ( MGI:2386853)
Sequence:	sense strand is shown >T8812 TGCAGGCACAATTCTAGTGCACGCGACCTCTCTTGCTGGTGGCCTCTGGACCGCGCGATGACTGAACGGT GACCTCTGGGTCTGAGGACGAGAGTTGAAGATGAAGGAAGCTTGCTCTTCCAGCTCTCATGTTCCTGTCA GCGACAGCAAGTACATTCTGAAGTCAGAACTCTTAAGCCTGCTCAAAACCTACAACTGCTACCATGAAGG CAGGAGCTTTCAGCTAAGACACCGAGAGGAAGAAGGGACTCTGATCATCGAAGGACTCCTCAACATTGCC TGGGGACTCAGACGACCCATCCGGCTGCAGATGCAGGATGATCGGGAGCGAGTGCACCTGCCGTCTGCCA CATGGGTGCCTGAGAGGCTCAGCTACCTCCAAAAGGAAGCTTCACCCCAGGACAGCAAGGTCCCCACTGA AGAGCCTGGCACTCAACCTGCAAACAAGGCAGAGGTTTCCGGAGATAGCTCAGGAGCCCTGGAGGGGGAA GAGGAGGAGGTCCCACAGCTTATGCGGACAAAGAGCGATGCCAGCTGCATAATCCAGAGGAGGTCCAAAT CCCGGGCACCAAGCGAAGCCCAGAAGATACGACGGCACCGGTTCTCCATCAATGGACATTTTTATAACCA CAAGACCTCAGTGTTCACTCCTGCCTATGGGTCTGTGACCAACGTCCGGGTTAACAGCACTATGACCACC CAGCAGGTGCTCACCCTGCTACTAAACAAGTTCCGGGTAGAAGATGGTCCCAGTGAGTTTGCACTCTACA CCGTCCATGAGTCTGGGGAGCAGACAAAATTAAAAGATTGCGAGTACCCACTGATATCCAGAATCCTGCA TGGGCCCTGTGAGAAAATTGTCAAGATCTTCTTGATGGAAGCTGACTTGAGCGAGGAGGTGCCCCATGAT GTTGCCCAGTACATTAAGTTCGAAATGCCGGTGCTGGACAGTTTTGTTGAAAAATTAAAAGAAGAGGAAG AAATAGAAATAATCAAACTGACCATGAAGTTCCAAGCCTTGCGTCTAACAATGCTGCAGCGTCTGGAACA GCTGGTGGAGGCCAAGTAACTAGCCAGAGCTTGCCTTTTGTGATGCCTGCAGTAGCAAGTGTGCATGGAG TCCCAGGGACCCAGCTCTAACTGATCGCTGACCAATGGTTGCTGCCCAGTACCCATGCTCATTCCACCTC TCCACGGACGACATAGCATCCTGCATCTCTAGTCTTGGTACCACGGTGAGCATGCACAGGCACACCCAGG AACTTGCTGGCTAGGATCTATGCGCAAAGCTGGCCTACGTGGTAATAAAGAGCCTTGGAACTGTGGTACC AGCCACATTGTATGGTTGATTAGTATGGCCAGCCTTGTTCCTGTGTATTTCCAGCTTGAGAGAGCTGTAT AGCACCTATCCCTGGAGAATGGGTTTCATCTGCTTGCCCAGTGCCTGTCCCTGAGCCTCTGCATGGCCAC AAGTCCCTGCCCTTATCACCCATGCGAACAGAAACTCTGTCTTCCCTTGCTTCTCATTGCCTCTCAGGAA CCATTCTGGAGCAGGAGCTGCCAACAGTCGTGAGATATGGCCTGCTTCTAACTATTGCTTCACCATGATA TCCTGGCTTCCGAGCCTCCTTTCCCAGCCAACATTCCTGGCCTAGAGGTTAGATCGGGACTAGGACTACC AAGATCTCCTCAGCTCTTGGCACAAATCACCCTTAACTTGTGAGTGTGATTCAAAGAAATCCTTGAAAGG AGTTGTCTTCAAGAAATATGTCCATTGAATTATGTTTCGGAAGTTGATTCATGTAGCTGACAGTGGATCC AGAAGGGTCAGTCCCCTTCACTCCATCTTGTGGATGTCTATATATGTCTCCTGCTTTCCTTGAGACAGAG CTTGTTTCTCTCTAGTGGATAGGGACAGCTCTCAATACTTCCTACCAAAGCTCTGCAAAACATGGAAAAA CGTCTATTTGAGGACAAAAGGCTTCTAATCCCTTAGATACAGATACAGAACCACTTGTTTGCTGGCTGAT TGAAACCTCTGGAATGAATGAGAAAACTATGCAGTGGTTTATATCAGTTTTGGGAATGATTGTCTAACAG CAAACTAAAGGCCAGCTTCTTCTCCTGAGGTCTGTGGACAGTGGATTTAAAGAGAAGTATGATGAAGGCC TTCGAAGACAGAGTTCCATCTTCAAGGCAGTGCATGTAGAAGCCACTCATGGAGCCCTGAAGAGCAGAGT GTGAGAACAGCTGTGCAGGGGGAAGCCACAAGGCAGGGTAGATGTCGCCTCTAGATTTTCCAGACCCAGC TCTGTTTCACAGAGATGCTCAGCTGCCAAGAGAGGTCTCTTGCCTCCTCCCCTCTTTGGCACTGTACTTT CTTCGGTGCTTAACAAACGCCTGTTGTGTTGAGCAGAAACTGCAGGACCAACCTTTCTTGTGCTTCTTCT TGTTGTGCTTATTGCCTTGATCTGTTTTTCTAATCCCCGAAGCTGTTGTACCACGCTATTAAGAGCTGCA GCTGACAGAGGAACCACCCACCTGTTGGAACCTGTCCACATTGACAGGACTTCTTCACCTCGTGCACGGT CTGAGAATTGGTGTGACGCCTGAGGCAAGGCTGCCCTGCTTTAGAACACAAGAAAGGACTCACTGGTGGT GGCCTGTGGATGGCCTGAGCAATAAAGTCATCCTAAATTGCAGAATTTTAGCATCAGAAGTGCAAAGGAA TCTTCTAGCCAAAGACTCTGAAATTGAAAACCTGAACTGGATTTTTAGCCTCAAGACATCTGCCTGTCCG GTGCTCAGAGGTAATTAACTGACTTGACTGTTTTATGCCAGGCCTGTGCTAGACCCTGGAGGAACAAAGA CAGAGCCTTGGTAATTCCTCTTCTGGAGGAGCAGACTTGGAGACAGTTATGCGCCTGATATTGAGTAGAC AGCTAAGAGTGAATGGTACTGAATGGTACTGTAAGCCACGAGACAGCTTCCGTCCCTGACCCAGGAACTC ACTCAGTGCTGGGGGAACCACTGACACAGGAAACCCTGGAAGTGGCCACCAACTTGGTGGTGTTTCACAG CTGTCCAAATATTACTTAAGGCTTTTTAAAATAAGTTTTACTTTCAAACTATTATCAAAACTAATTTCTG AATGACTAGAGTTTGGAAAAACTCTAGCAAGTTGCACAGAGCAGTGCTCTCTGCTGTGTTCCTCTGAAGT GTTCACAAATGTTAGACTGACCTGCCACATCTGCTCTTAGAGGACAACTGGAGAACCAACCTTTGCAGGG CATAACAACCCCAGAACTTGAGACTATGGAGGCTGTGGCTTCACAGTGCGCTTGGGCTATCACCTGCGAT GGAGGTCACAGCCTTCTGGTGTGTTCTCCACTTCTCAGATGATAAAGGTATCTATAGATAAAGTGAAGAT CCCGGTTAGAAAGGACTGTCTTCCAAAATAGATCTGTCCTCAGAGCTTCACATAGTAAACAGCACAGGTC GAAATCAGTGTGATTACAAGATTGCAAAAGTCCCGAGCTCAGGATAAACAGGGTTGTTTGTCCAGATCAA ATGACCAGAACGGATCGGAAACAGGAGTAGGGGAAAGACTTGTTTTTCTAAAGCAATAATAACCTTTAGA CTTTCTCAGTGACAAAACAAAGTCTTTCACCCAGAATACATCTGGCTTCTTGGTCACAGGGCAAGGAGAG AGGGCTTATTTACTCTACTCAGGTGATCTGGTAGGAACTGGAATTATGCCAGCTGCACTGTGTGAACACA GCTGCAGAGTGACAGGCCATGGAATGTCCTTATCAGGAGTACTCTGACTACCAGGTCCTCAGGCCCTCTG ACCCTCATCAGTGGCAAGCAGGAAGTCTGCTAGGTCCCAGCCAGGCAGTAGCACGTTTCAAAACAACCAG ACGCATGGACTATATTTAGGCCCCAAGAACTACACTTGGGTATTAGCCAGCTGCTGACGTGCCAGGAGCC TCACAGAGCCTGAGGTGGGGTTCTGTGCCCTCCAGCAGGGTTGGGACAATGGTTTGCTGCTAATCTAAGA ACATGGCAATTCCCAGGGTTTCCTTTTTCATCCAGCCCAAGGGGCATAAGACAGTGCCTCAGGCTTGTCT AACTGACTCATTGTCCAGGCTCCTGGGCTGAAGGACCCACTTTGAGAGAAATAGAGCATGTTTGATCCCA TACTCAGGCCATTCAAATGACCCGTCCTGCTGTCCCTGGACTGTGGGTCAGTTCTGCCCATGACAGCAGC ACTCCTCACTGATACAGGGCTTACTCATGTACATGTACGTACACCTGCATGTGTAGCAAAACACTTATGT GAAGACCAGGGTCTAAGGAACAAAACCACAGAAAAGGGAAATTACTTTGCTAGGCTGTTTCTCAGCTGTT GTTGTTGTTGTTGTTGTTGTTCTACATTTCTTGTTCTATAATGTTCTTTAAATATTCAACCCACTCTGGG TAAGTGGCCCCTGTGGTGCAGATCTCCAGAGAAGCTACAGTCACTGAACTAAGACTCCCCAGAAAGAAAA TCTGCTAGGTGCCCAGGCTGTGGCCTCTCCTCTTACCCTTTGTCTTCTTCTCCCTTTGTCTGAGGACTAC ATGTGACTGTTCCTCATAATTTAATTTACTTAAAAATGACGTGGTTCCAAAACTTGACTCTGTTAAGTGT GTGCATGCGGCTGTTTCGCCATATCTGGTCCTCGTGTGTGAGAACCAGCTGCCAAAGGCAGAAGGTGCTG TGAATCGAACCGGAGGCACAGATCGACCCCAGAGTGCAGAGTGTAACTTATGGATTCACAGGCTTCCTTC CGTGGCCTGAGAAGCAACAAGGCAGAAGATCCTCATATCTCAAGTAGGAGTAAGACGTTTCTACATCACT CCAGACAAGTGTGGGCTGCAGTTAACCAGTATATACCACATCTCTCTCCAAAGCTGTTGTCACATCCAGT ATGAGTTAAGAATACTGTGTGTCAGTGACCAGCACAGAGTGCTTGGGTATCCTAACAGCTTTGTCTCACA GGGCTGGTGATGTGGGCCAAGGTCAGCTGGTAGGCAATATGGCAGGTATAGTGAGCATGCCTTTCATCAC GTCAGACTGGATTCTACACTGGGGAAGTTACTTGCCTCAATTTCCCCATCAGTCAGATAGGGGTAAACAT GGTTCCTAGCTCTCAAGGTTACTGAGAGTCATAGCTTTATTGCCATAACAAGGCCAGAAATCTCAGCATC ACAGGAGACTATACATCTCTCAGAAACTGTGGGACTATATAGTACCCATGGTGTGTTCTGATCGATCCTG GATCCCCGGACTCCTTGGGACATGCTATTGAAGCAGAGTGACGGTGGGAGCGTGTGTATCGGGGCTGCTT CTGGGCCACTGTGTTGCCTCCATCCGTTCTTGAGCAGTGTTGAGTCCCCTAGTAAAGTCCTCATCTGGAG CTACACACTCTAGGTTGTGTGGGGCTCAACCTTCCTAATGCTCCAATCCTTTAATACACTTCCTCATGGT GTGAAGACCTCCCGACCATAATAATTACTTTTGTTGCTGCTTCGCAACTGTAATTCTGCTACTGTTATGT ATGAATCGCAGTGTAAATATCTGATATTACCAGTGGTCTTCGTCGACCCCTGAAAGGGATCATCAGACCC CAAAGGGGTTGTGACAACCCACAGGTTGAGAATTGCTGCACTGGGGACTCTGAGAATTGCCCCCACCCCC CAATACAGAAAAGAACAAAGATCAAAGAAGATGTCCCACACAGCCATTATGGTCGTGCTATGGTAGTGTT AAAGAACTTAAAGGTTCCTCAGACCTGGCTGAAGTGTAGCAATCGGATGTGTTATGCTAACATATGACCC AGGGCATGGGGCTTTGATTGCTCACACCGCTTTTCTTTGCCACTTTGATGCTCGTGTCCTTTCCTGGCCC AGGCCTCTCCCCAGGCTTCCCTCCCAGTCTTTCCACTCACAGTGGCTTTGCTCCTTTCTTTCCTCACATT TGTCTCCTTCCAAAACGCCACCACTCTTTGACTGCCTGCCTAATCTCAATCCCTTTGGGCCTTTTGTAGC TGATGTAGCTGTTTTTTCTATACAGGCAAGAAGGATGCTCTCTCTACCTTTAAGTGAGGCAACAAAGACT TACGACTCATGACTAGTAGTCTCAGTGCATTTTGGCTCTTGCATTTTTTCCTAGTCCACCAGTGCCCACC ACCATTGCTCACCGCTGGCACCAACCACGTATGACAACAGTTTAGTAATAAAATTAGCAATAAATCATGA AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6409692 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6409692 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19771 same embryo
	EMAGE:19769 same embryo
	EMAGE:19766 same embryo
	EMAGE:19768 same embryo
	EMAGE:19770 same embryo
	EurExpress:euxassay_006256 same experiment
	MGI:4827635 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source