EMAGE mouse gene expression spatial database

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EMAGE:20154

Pcyox1 prenylcysteine oxidase 1 ( MGI:1914131)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012457. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012457_01	euxassay_012457_02	euxassay_012457_03	euxassay_012457_04

euxassay_012457_05	euxassay_012457_06	euxassay_012457_07	euxassay_012457_08	euxassay_012457_09

euxassay_012457_10	euxassay_012457_11	euxassay_012457_12	euxassay_012457_13	euxassay_012457_14

euxassay_012457_15	euxassay_012457_16	euxassay_012457_17	euxassay_012457_18	euxassay_012457_19

euxassay_012457_20	euxassay_012457_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20154_wholemount_strong.wlz

20154_wholemount_moderate.wlz

20154_wholemount_weak.wlz

20154_wholemount_possible.wlz

20154_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20154_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pituitary gland

weak

regional

weak expression: see section 09 10 11 12 13 14

vibrissa

weak

regional

weak expression: see section 01 02 18 19

diencephalon floor plate

weak

regional

weak expression: see section 13

medulla oblongata floor plate

weak

regional

weak expression: see section 13

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 15 16 weak expression: see section 08

metencephalon floor plate

weak

regional

weak expression: see section 12 13

pons mantle layer

weak

regional

weak expression: see section 07 17

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 16 17

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 16 17 18 19 20 21

ventral grey horn

weak

regional

weak expression: see section 10 11 12 13 14 15

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31331
Entity Detected:	Pcyox1, prenylcysteine oxidase 1 ( MGI:1914131)
Sequence:	sense strand is shown >T31331 TGCCTCGGCTTTATCAGGGATAGATTCAGAGATAGAAGCAGAGATAGCCTGTGATTTGGAACCAAATATA TAAACTATTAACTTGAGAAACTATTTAATATATAAACTATTAACTTGAGGAGGTCTGTTTTTCTATACTG TGGGGATTGAACCCAGGACCCCGAGTATCCCAGACAAATGCTCTACCACTGAGCTACATTGCCAGCCTCA AGAGCTTCAGGATCGACATCCAGAGAATGGAATTTCCTAGGTTGAAGATGGGGTCTTGGCTCTAAAGCTT TGTCAAGATCAAGTTCCTCGCATGCAGCCAAGGGTGAGCAGGTCCTGGGTGTCAGATAGAGAGCTTTGAG TGCCTTAAAAGTGCTAAGCAACACAAATCAAAACCACAGCAGACCGGTGCCAGAGGTGACTGGCTGCTCG ATGGTTAATCAGCTGTGGTCTCCAAGGGAACGAACACTAGAGCAGAGGCGGCCTGAGTGAGCCCAGAGCA GTTGGCCTTAGAACCAGGTCTTCATGGCTGCGTGAGTTCAGAGGGACGCGTATCAGATGCTGGGCATGCG CAGTGAGGGGCCGTTGCTGAAATCTTAATACAGTGGCGCTCAGTTGTAGCTGCACATGGGAGTCAGATTT CTCTTACGTAGACAAGCTCAAGAAATCTGGTGTTTAGTCCACTGCTCATAGGTCTCCAGTGACTATCATA GCCACTAGTATTAAGAGGGAAAAAAAGTAATTTTGCCTGCTTTGGTGGTACTTGCCTGTCATCCATACCT TTGTGACAAAGCGAGAGGATCAAAAGTTCAGAGCCAGCCTGGGCTGTGGCAACACCCTATCTCAAGGAAT AAAAATCAAAACTCTTCACCAGGTTATAGCGTTGCTTCAATAAAGCGGTCTTAAAAAGCCTGATTCTTAA AAATTATTTTTCCCTACTCTAGCTGTAGGAATTAATAATGTGTGGAGAAAGCAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3486530), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 21265. Forward Primer - name:021265_F_IRAV51-54_I24_Pcyox1, sequence:TGCCTCGGCTTTATCAGG; Reverse Primer - name:021265_R_SP6_IRAV51-54_I24_Pcyox1, sequence:CGCTGCTTTCTCCACACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20149 same embryo
	EMAGE:20152 same embryo
	EMAGE:20153 same embryo
	EMAGE:20151 same embryo
	EMAGE:20150 same embryo
	EurExpress:euxassay_012457 same experiment
	MGI:4827106 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source