EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:6838

Timm23 translocase of inner mitochondrial membrane 23 homolog (yeast) ( MGI:1858317)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002670. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002670_01	euxassay_002670_02	euxassay_002670_03	euxassay_002670_04

euxassay_002670_05	euxassay_002670_06	euxassay_002670_07	euxassay_002670_08	euxassay_002670_09

euxassay_002670_10	euxassay_002670_11	euxassay_002670_12	euxassay_002670_13	euxassay_002670_14

euxassay_002670_15	euxassay_002670_16	euxassay_002670_17	euxassay_002670_18	euxassay_002670_19

euxassay_002670_20	euxassay_002670_21	euxassay_002670_22	euxassay_002670_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6838_wholemount_strong.wlz

6838_wholemount_moderate.wlz

6838_wholemount_weak.wlz

6838_wholemount_possible.wlz

6838_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6838_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 16 17 18

telencephalon ventricular layer

moderate

regional

moderate expression: see section 09 10 14 15 weak expression: see section 01 02 03 04 05 06 07 08 16 17 18 19 20 21

medulla oblongata basal plate

moderate

regional

moderate expression: see section 07 08 14 15

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 weak expression: see section 06 14

ventral grey horn

moderate

regional

moderate expression: see section 13 weak expression: see section 08 09 10 11 12

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 14 15

lower jaw molar

moderate

regional

moderate expression: see section 08 09 17 weak expression: see section 06 07

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

upper jaw molar

moderate

regional

moderate expression: see section 08 09 17 weak expression: see section 06 07

orbito-sphenoid

strong

regional

strong expression: see section 20 moderate expression: see section 01 02 03 04 05 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5038
Entity Detected:	Timm23, translocase of inner mitochondrial membrane 23 homolog (yeast) ( MGI:1858317)
Sequence:	sense strand is shown >T5038 AGGTGGCAGGAGCAACGCGGGTCTTCCCGCTGTTGCTTGTCGCGGCCACGGCCGAGCATACGTCCCCGGC CTGAGGTGGTGGTGGCGAGCCCACCGCCGTTTGCTGCGACCTCATGGAAGGTGGCGGAGGAAGTAGCAAC AAATCCACCAGCGGGTTAGCTGGCTTCTTCGGAGCCGGAGGAGCGGGTTACTCGAACGCTGATTTGGCCG GCGTCCCGCTGACTGGTATGAACCCCCTGTCTCCTTATTTAAATGTGGATCCACGCTATCTCGTTCAGGA TACTGATGAATTTATTTTGCCAACTGGAGCTAATAAAACCCGAGGCAGATTTGAACTAGCTTTCTTTACC ATTGGAGGATGTTGCATGACAGGGGCCGCATTCGGGGCAATGAACGGTCTTCGTTTAGGATTGAAGGAAA CCCAGAGCATGGCCTGGTCCAAACCAAGAAATGTACAGATTTTGAATATGGTGACTAGGCAAGGAGCACT TTGGGCTAATACTCTAGGCTCCCTGGCTTTGCTCTATAGTGCTTTTGGTGTTATCATTGAGAAAACACGG GGTGCAGAAGATGACCTCAACACAGTAGCAGCTGGAACCATGACCGGGATGTTGTATAAATGTACAGGTG GTCTTCGAGGAATAGCACGCGGTGGCCTGGCAGGACTGACACTCACCAGTCTCTATGCACTGTATAACAA CTGGGAGCACATGAAAGGTTCCCTGCTCCAACAGTCACTCTGAAGATTTTTGGCACCTCAGGAATGGGGG ACACTTCATAGTCATCCAGATCAATTAATAAGCCAGTCTGGAGTTGCTTTCTGTCTTGTACCTACAGTTA CTTTGAACAATTGGAGACTCTGATTTGCTGTGACAAAGATCATGGATGGTTAGCCTGGACTGCACTGCTT GCCCTCAGTGAGTGGAAGCCAACCCCTTGGTGGCTCACTGAGTATGTGTTCTCTTCTCCTTTGGAGATGA TCTTGCACCCCAAACTGGAAAACCACTTACTCTCCAAATTCAGGCCATACTTTTTATTACCATGTTAATT CATTTAAAAAATCTAAAACTTAAATGTTGCCCTGTTTTCCAAATAAAGGGTGAAAACAGAACCAAAGTCA TAATTCCAACATAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5290493 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5290493 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6836 same embryo
	EMAGE:6841 same embryo
	EMAGE:6837 same embryo
	EMAGE:6840 same embryo
	EMAGE:6839 same embryo
	EurExpress:euxassay_002670 same experiment
	MGI:4828718 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source