EMAGE mouse gene expression spatial database

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EMAGE:6970

Carns1 carnosine synthase 1 ( MGI:2147595)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002476. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002476_01	euxassay_002476_02	euxassay_002476_03	euxassay_002476_04

euxassay_002476_05	euxassay_002476_06	euxassay_002476_07	euxassay_002476_08	euxassay_002476_09

euxassay_002476_10	euxassay_002476_11	euxassay_002476_12	euxassay_002476_13	euxassay_002476_14

euxassay_002476_15	euxassay_002476_16	euxassay_002476_17	euxassay_002476_18	euxassay_002476_19

euxassay_002476_20	euxassay_002476_21	euxassay_002476_22	euxassay_002476_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6970_wholemount_strong.wlz

6970_wholemount_moderate.wlz

6970_wholemount_weak.wlz

6970_wholemount_possible.wlz

6970_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6970_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 08 09 10

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 09 15 moderate expression: see section 08 16

pons mantle layer

moderate

regional

moderate expression: see section 07 17

facial vii ganglion

strong

regional

strong expression: see section 03 04 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 weak expression: see section 07 17 18

ventral grey horn

strong

regional

strong expression: see section 09 14 16 moderate expression: see section 10 11 12 13 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 09 15

cervical ganglion

moderate

regional

moderate expression: see section 08 16

thoracic ganglion

strong

regional

strong expression: see section 13 14

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 11 12 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5158
Entity Detected:	Carns1, carnosine synthase 1 ( MGI:2147595)
Sequence:	sense strand is shown >T5158 CCACGCGTCCGGCGCAGGTGGCGCTTCTGGAGCAGGGCATCAAAGAAGCAGCCGAAGGCGCGTTGGCCGC TGTGCTGGCCCTGGAGGCTGGACTGAGTGTGGAGCAGCGTGGTGGACGCCAAGTGCACACCGACTTCCTC GCTGCACCTGGTGGAATCAGACCCCAACCACTTTGCATCCCAACTGGTACAGACCTTTATCCACTTTGAT GTGACTGAGCACCGGAGGGATGAGGAGAATGCGCTGCTGCTGGCTGAGCTGGTACGGGCCCGGAACCTGA AGCTAGATGGCTGCTTCTCCTTCTGGGATGACTGCTTGGTGCTTACTGCTCTCCTCTGCCGTGAGCTGGG TCTGCCCTGCAGCCCCCCAGCTGCCATGTGCTTGGCAAAGCAGAAGAGCCGCACACAGCTACATCTGTTG CGGTGCCAGGGCCCACCCTGGCCTTCGACCTCTCTCCATGCTGTGGCCTGTTGCCCATTGGAGAATGAGG CTGATGTGGAGAGAGCCATTTACCAGGTACCCCTGCCAGGTGTGATGAAACTGGAATTTGGGTCAGGAGC AGTAGGCGTGCAGCTGGTGAAGGATGGGCCTCAGTGCCGTGAGCACTTTTCCCGGATCCTCCATGACCTG CAGGGTGAGGCTGACCACCCTGGCATCGGGCTGGGCTGGGGCAATGCTATGCTGCTGATGGAGTTTGTTG AGGGCACAGAGCATGACGTGGACCTGGTGGTGTTCGGTGGACGGTTGCTGGCTGCTTTCGTCTCCGATAA TGGCCCCACAAGGCTGCCTGGCTTCACAGAGACTGCAGCCTGCATGCCCACTGGGCTAGCACCAGAGCAG GAGGCCCAGATGGTGCAGGCAGCCTTTCGCTGCTGCCTGGGCTGTGGGCTGCTGGATGGGGTTTTCAATG TGGAGCTCAAGATGACTGGGGCTGGGCCAAGGCTCATCGAGATTAACCCTCGAATGGGAGGCTTCTACCT TCGAGACTGGATCCTAGAGCTCTATGGTGTGGATTTGCTTCTGGCTTCTACAATGGTGGCCTGTGGCCTG CAGCCTGCCTTGCCTGCTCACCCTCGTGCCCGTGGCTACCTAGTGGGCATCATGTGCCTGGTGTCCCAGC ACCTACAGCTGCTGAGTTCCCCGAGCAGTAGAGAGACTCTGCAAACCCTCCATGACCAGGGGCAATTACG ACTCAACCTGCTGGAGGAGGCCCTGATACCTGGCCAGTATGAGGAGCCCTACTGCAATGTGGCCTGTGCT GGGCCCAGCCCTGCTGAGGCCTGTCACCGCCTGCTAGGCATCTGTCAGGGCTTGGGCATTGACAGGCCCA ACTACCCTGTTGCCCATTTCCTGTCTCATTTCAAATAGCACTGGGGTCAAGGAGCAGCAGGGATGCAGTG CCAGGAGGAGGGGCCGTGGGGCTATATTTCCCTGTCCCTCTCTCTGTCACTTTGCCCCAACCCAGGGTCA GCATGCTACCACAACTCAGGCCTATTCCAGAACAGTAGAGCCATTGTGACACCAAAGTCTTCTGGATTCA AGGACCATAAAACAAGTATCTGGAATTTGCTGGGTCCTCTTGCCTTCCAGAAAGCCTCCATCTAGCCCCA GGTACCCCCAGGACTCCAGCCTTTAAAGAACTAACAAATAGCAGCTTCTTCACTGGCACATCAGTGCCCA GCCCAGATGGGGGTGGGTGAAACCCAGGCTCCTCCTCTCTTACCCCTCCACCGCCTAGGTCCAGCTCTTC CTGTCACCTACACAAGGAGAGAACCTTCACCAGCCCCTAGCCTTGGACAAAGGCCATGAAATGGGAGATT AGCACACTAGCCTTTATGCAAAACCCAGGACAGTTGTAGAGCCTGCTGAAAATGTCTGGCCGTGCTTCAC TGTGTCTGTTGGCCTGAGCAGTAGAAGCAAATGTCTGTGCTCAGCACTAGGAGTAAACATTTCCCTTTCA TGCTTTCAGGCAGACCCTAGTGCCCCTATGTTACAGATGAGGAAGCAGGCTCAGAGAGAGAAAAAAAAAA AAGACTGCCTCCAAGTTCCTATTATAGTGGATGTAGACAGAATGCCAAGGCCAACCTTCTCTGTTTACTG TTTTAAACGCTCAGTTAGCCTCCAGGTCCTCTCTCCTTTCTATCTTCATTTATAGGCAGCACTCAGACTC CTAAACACCTGCCTCCCATACACCACACACGATCTCTACCAGCTTTGTTGCTTTGGCCTCTGGCATTCCT AGCTAGCTTGGCCCTGGGACTTCTGTGTACACCTTTCCTCATTTTAGGGAGCTAGCTACTCTGAAAACAC CTGTCTTTGCTCAGGAGCCGAGGACAGCTCCTGAAACCTGCCTACCTAAATAAAGACTTGTTAACTAGTG AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5345551 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5345551 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6972 same embryo
	EMAGE:6973 same embryo
	EMAGE:6969 same embryo
	EMAGE:6971 same embryo
	EMAGE:6974 same embryo
	EurExpress:euxassay_002476 same experiment
	MGI:4823346 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source