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EMAGE:7083

Syt2 synaptotagmin II ( MGI:99666)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7083 EMAGE:7083 EMAGE:7083 EMAGE:7083 EMAGE:7083
"Pseudo-wholemount" of euxassay_002635. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002635_01 euxassay_002635_02 euxassay_002635_03 euxassay_002635_04
EMAGE:7083 EMAGE:7083 EMAGE:7083 EMAGE:7083 EMAGE:7083
euxassay_002635_05 euxassay_002635_06 euxassay_002635_07 euxassay_002635_08 euxassay_002635_09
EMAGE:7083 EMAGE:7083 EMAGE:7083 EMAGE:7083 EMAGE:7083
euxassay_002635_10 euxassay_002635_11 euxassay_002635_12 euxassay_002635_13 euxassay_002635_14
EMAGE:7083 EMAGE:7083 EMAGE:7083 EMAGE:7083 EMAGE:7083
euxassay_002635_15 euxassay_002635_16 euxassay_002635_17 euxassay_002635_18 euxassay_002635_19
EMAGE:7083 EMAGE:7083 EMAGE:7083 EMAGE:7083 EMAGE:7083
euxassay_002635_20 euxassay_002635_21 euxassay_002635_22 euxassay_002635_23 euxassay_002635_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7083Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7083_wholemount_strong.wlz
7083_wholemount_moderate.wlz
7083_wholemount_weak.wlz
7083_wholemount_possible.wlz
7083_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7083_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
olfactory cortex marginal layer
strong strong
regionalstrong expression: see section 09 10 11 12 15 16 17
medulla oblongata basal plate
strong strong
regionalstrong expression: see section 09 10 11 15 16 17
metencephalon basal plate
strong strong
regionalstrong expression: see section 08 09 19
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 07 08 20 21
trigeminal v ganglion
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 18 19 20 21 22 23
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 07 08 20
dorsal root ganglion
strong strong
regionalstrong expression: see section 09 11 14 15 16 18 19 20 moderate expression: see section 10 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5287
Entity Detected:Syt2, synaptotagmin II ( MGI:99666)
Sequence:sense strand is shown

>T5287
GCGGGAGGAGAGCCGCAGCGCTGGCCGGCGCGGGAGTGGGAAGAGGGGACCTGGACTTCGGGGCGGCAGA
CTCCCGCTGCCTTCTGCACCAGCCCACGCCGAATCGGCTCCCGGACCGTCCCCGGCTATCCCCTCTGCCA
CCATGAGAAACATCTTCAAGAGGAACCAGGAGCCAAATGTGGCTCCGGCCACCACCACTGCCACAATGCC
CCTTGCACCCGTCGCACCTGCCGACAACTCTACAGAGAGCACGGGTCCTGGGGAGAGCCAAGAAGACATG
TTCGCCAAGCTGAAGGAGAAATTCTTCAATGAGATCAACAAGATCCCCTTGCCCCCCTGGGCTCTGATCG
CCATGGCTGTGGTTGCTGGCCTCCTGCTGCTCACCTGTTGCTTCTGCATCTGTAAGAAGTGCTGCTGCAA
GAAGAAGAAGAACAAGAAGGAGAAGGGCAAAGGCATGAAGAACGCCATGAACATGAAGGACATGAAAGGG
GGCCAGGATGACGATGATGCAGAGACAGGCCTGACTGAAGGAGAAGGTGAAGGCGAGGAGGAGAAAGAGC
CAGAGAACCTGGGCAAATTGCAGTTTTCTCTGGACTATGATTTCCAGGCCAACCAGCTCACCGTGGGTGT
CCTGCAGGCTGCGGAACTCCCAGCCCTGGACATGGGTGGCACATCAGACCCTTATGTCAAAGTCTTCCTC
CTCCCAGACAAGAAGAAGAAATATGAGACTAAGGTGCATCGGAAGACGCTGAACCCAGCCTTCAATGAGA
CATTCACTTTCAAGGTGCCATACCAGGAGTTAGGAGGCAAGACCCTGGTGATGGCAATCTATGACTTTGA
CCGCTTCTCTAAGCATGACATCATCGGGGAGGTGAAGGTACCCATGAACACAGTGGACCTTGGCCAGCCC
ATCGAGGAATGGAGAGACCTACAAGGCGGAGAGAAGGAAGAGCCAGAGAAGTTGGGTGACATCTGTACCT
CCTTGCGCTACGTGCCCACAGCTGGGAAGCTCACCGTCTGTATCCTGGAGGCCAAGAACCTGAAGAAGAT
GGATGTAGGGGGCCTTTCAGACCCCTATGTGAAGATCCACCTGATGCAGAACGGTAAGAGACTCAAGAAG
AAGAAGACGACAGTGAAGAAGAAGACCCTGAACCCCTACTTCAACGAGTCCTTCAGCTTCGAGATCCCCT
TTGAGCAGATCCAGAAAGTCCAGGTGGTCGTCACCGTGCTAGACTACGACAAACTGGGCAAGAATGAAGC
CATCGGAAAGATCTTTGTAGGCAGCAACGCCACAGGCACCGAGTTGCGGCACTGGTCCGACATGCTGGCC
AACCCTCGGAGGCCCATTGCCCAGTGGCACTCTCTTAAGCCTGAGGAAGAAGTGGATGCTCTTCTGGGCA
AGAACAAGTAGGCTCCAGCGGCCGGGGCCACGCCCCTAAGGAGCCACGCCCCCGAGGCGCCACGCCCCCT
GAGGACACTGACGAGATCCAGAGCTATCAATACCTCAGTTACGCGACCTTAGAGGTTTCTTCATTTGTTT
GCGGTGTGTCCTGTTTTTCCTTCCTTTTTCTCTTTTTAAAGACCAACTTCCTTTTGGTGGCTGTGTGAAG
AGAGTCCCCTAAGAGGTGAAAGAAAAGCCTGGCTCTGTTATTGACCCCGGAGCGGCCCTTGTTGCATGCC
CTTTCACGGTTTCCCCCTTACCCCAAGTGGGGCCCTCTACTGTCAGACAGTTGAAGCACTAACTGCTTTT
CCTGGGTTTTGGACCAACAACATGGCAAGCACATTCTGTTTCTTGACTGTGAAGGCAACATAGTGGCCAG
CATTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTACACCTGTATGTGCCCATCCAT
CCCCATCTGCCTGTTTTGAACATCTCTCTTCATTTTCTGGAATGAGTCATGGACAGTGAAGCCATGTGAG
AGGAGAATGTCTTCAGAGACTCCAAGGGAAAGCAAGCCCACTGCCTAAGCGGTCAGATGCCAGAAGAGAG
CTTGCGGATGGCCATTTGGAAGGCGGCGAGATGTGATACTGGATGGGTTCAGAACTGGGACCAAAGCCCT
TGATGCCATCATAGACACCCTCTTGTCAGTCATGGCTTTCCCCCCTGGAGTGGGGTTTTGGGGACATTCG
TGGAAATCAACTATTCACCAGATTTGCCAGACTCTACCAGGGACTGCCAGCCCTGGGATGTCACTCCTGT
TGACAACATCAAACTTTGAAGAAACAGAAGTCCCATTACTCAGAAAGCCTAGAATTAAGCTCCTGGATCT
TTGCCTCGTCCCTCGTCCCTGCAGAGTACAAGCAGCGCGGTCATTTGCATTCACGCACCATCTTGCTTTT
GCCTCTGTTTCCCTTTTTGTGTAAGTGGAAAAATACCATCTGAAGACAAGTGCTTTGCACAGAGCCAGAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5362352 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5362352 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7086 same embryo
 EMAGE:7087 same embryo
 EMAGE:7085 same embryo
 EMAGE:7084 same embryo
 EurExpress:euxassay_002635 same experiment
 MGI:4828571 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS