EMAGE mouse gene expression spatial database

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EMAGE:7117

Tmem179 transmembrane protein 179 ( MGI:2144891)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002587. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002587_01	euxassay_002587_02	euxassay_002587_03	euxassay_002587_04

euxassay_002587_05	euxassay_002587_06	euxassay_002587_07	euxassay_002587_08	euxassay_002587_09

euxassay_002587_10	euxassay_002587_11	euxassay_002587_12	euxassay_002587_13	euxassay_002587_14

euxassay_002587_15	euxassay_002587_16	euxassay_002587_17	euxassay_002587_18	euxassay_002587_19

euxassay_002587_20	euxassay_002587_21	euxassay_002587_22	euxassay_002587_23	euxassay_002587_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7117_wholemount_strong.wlz

7117_wholemount_moderate.wlz

7117_wholemount_weak.wlz

7117_wholemount_possible.wlz

7117_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7117_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

strong

regional

strong expression: see section 05 06 13 14 15 moderate expression: see section 01 02 03 04 07 08 09 10 11 12 16 17 18 19 20 21 22 23 24

telencephalon marginal layer

strong

regional

strong expression: see section 05 06 13 moderate expression: see section 04 07 08 09 10 11 12 14 15 16 17 21 22 23 24 weak expression: see section 18 19 20

olfactory cortex marginal layer

strong

regional

strong expression: see section 13 15 moderate expression: see section 09 10 11 12 16 17 18

hindbrain

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

moderate

regional

moderate expression: see section 07 20 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 08 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 18 19 20 21 22 23 weak expression: see section 03 04

spinal cord

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15 16

cervico-thoracic ganglion

weak

regional

weak expression: see section 10 18

cervical ganglion

weak

regional

weak expression: see section 09 18

dorsal root ganglion

moderate

regional

moderate expression: see section 17 18 19 weak expression: see section 09 10 11 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5243
Entity Detected:	Tmem179, transmembrane protein 179 ( MGI:2144891)
Sequence:	sense strand is shown >T5243 CAGCCTCCCACGCGGCTCGGGACCGGCCTGCGCGGCGCCGGAGACTCGCGGCCCGGCTTCTGGAGCGATC GCGTCCCTGTCCGGTGGCCTCGCCAGCGGCTCCTCTCATCCTGGCACCACCATGGCGCTCAACAATTTCC TCTTCGCGCAGTGCGTCTGTTACTTTCTGGCCTTCTTGTTCAGCTTCGTGGTGGTGGTGCCATTGTCCGA GAACGGCCACGACTTTCGTGGCCGCTGCCTACTCTTTACCGAGGGTATGTGGCTGAGTGCCAACCTGACG ATGCAGGGGCGGGAGCGCTTCACTGTGCAGGAGTGGGGCCCACCGGCTGCCTGCCGCTTCAGCCTGCTCG CCAGCCTCCTGTCACTGCTGCTGGCTGCTGCGCACGCATGGCGCACGCTCTTCTTCCTCTGCAAGGGACA CGAGGGCTCCTTCTTCTACGCCTTCCTGAACCTCCTGGTCAGTGCCTTTGTGGTCTTCCTGGTCTTCATT GCCAGCACCATCGTGAGTGTGGGCTTCACCATGTGGTGTGACACCATCACTGAGAAGGGCTCCACACCCC ACAGCTGCGAGGAGTTCCAGGAAACGGACTTGGAGCTGAACGTCGACAACTCTGCCTTCTACCATCAGTT TGCTATCGCCCAGTTTGGACTGTGGGCCTCCTGGCTGGCCTGGCTGGCGATCACCACTCTGGCTTTCCTG AAGGTTTATCACAACTACCGCCAGGAGGACCTGTTGGACAGCCTGGTTCACGAGAAGGAGCTTCTGCTAG CCAGGCCTACCTCCCGCACCTCCTTCCAGGGGGAGAAGAGTGCTGTCATCTAGGCTGTGCAAGGGTGGCC CCCTTTCCTGAGGCCTGCATGGGGAGGGGGCGCTGGGGACCCATCCAGGCTGGCCAACACAGCTCTGCAT GTGTCTCTTGGCCCCGCCCACCGTGAGCAGGGCACCTGAGTTCCCATGGAGAGCCTGACAGTGATGGGGC AGAGATCCAGAGACTTGGGGAGGGGGTGACTTCCAGCTTCCAGTCCTGGCCTTTGACGCATACTCCTTCA CCCGTGTGACCCTTTGTCTATATATGTGCATGCATGTGAGCATGTGTGTGGATGGGGGTGTGAGTGCAGA ACCGAGGTGCCGTCGCCGCCAGGTTAGCAGCCTTTGCACCACCACAGGCTCCAGCGCACGTGGTCCCTGC CCCTTCCATAAGGGGCTGACTTCCCATCCTATAGCACTCAGCCAAGGTTCTCAGAAGGCACTGGAGAAGG CAGCGGGCAGAGGATCCTGGTAGGAATGTGGGACCCTGGGCTGGGCTCCATCCCCTGCTCTGAGAGGCCA TCCTGGGCTGCTTGGCTGTCATGGGAGAGTTCCACTCTCCTCCCTTTGAGGCTTTCTAGGTGGCATGGTT CTCTGTGTTGCAAGGGTGAGGCCCCCATGGCCGAAGAGGCCCCGCCCATCTGGATCTCAGTGAGTGCGTT CAATGTTGCTGGCCCTAGTCTGCAGTTGTGCTGCAACGCTGTCCCCAATTTTCAAGCAAGGTGATCCTGA CCTGTGTCTGAGGCCAGTGTCCATCTGTGGCTGGTGTGTTTCCAGGTGGAAGGCACAAGCTTCCAGGCTC ACTGTGAGGAGCCTTTGTGTGTGTGTGTGTGTGTCAGTTGGGCCCTAAAAATTGAAGCCAGCAAGGATGG TCAGGGTGCTGTGCAAGGGGGTGCCTGTCTGAGGAGGTCAGGGTTGGGCCGACCGACACTCCAGTCTTCT CTGAGGGCTTTGGCCTTGATGAGCTCCCAGAAGAGGATCTGGTCTCCAAACTGACCTGGTCTCTTCTCCA CACCCTCACACAAGCCCTAATCTATGGGACATGGCGACACTGCCTAGCCTTGAGAATCCTCTGTTTTCCC AGGATTGGCTCCTTCCTCATCTGCCTCATATCCTTACTAAGGCTGTAGCCCTCTAGGACCTGAAGGAACA GGAGGCTGGATGGGGCCTCTGGGACCTAGGATCTGCCTCAGGTGTGGGTCTACCTTGGCAGCTGCCTCTT TGGATTCTGGAGAGTTAGTCTGGGATGCCTAGACTTGTCCCCCAGATGTTGGTGTTCCAGGGACTCCTGG GGAAGGGCCTCTGGGCCTAGGAGTGTTGGCAGGACCTGTTGGCTGAAGCCATGGGCAGGGGTGTCTGTCC AAGAGAGGTCTTATGGAATGGATGGCAAGAGACAGGCAGAGGCTGCAGAGAGGTCCCCAGGTTGCCTGCC AGACATCTCTGCTCCTTTCCAGCTTGCTACAGGGAAGCCCTGGTTTCAGTCTTCCTGGAGAAGCTGGTAG TGATGGTAGTGATGCCTCCGTGTGTCTGATCCTCAAGTCAAAATAAAATAAAGACGAAACATCTGAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5357484 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5357484 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7120 same embryo
	EMAGE:7116 same embryo
	EMAGE:7115 same embryo
	EMAGE:7118 same embryo
	EMAGE:7119 same embryo
	EurExpress:euxassay_002587 same experiment
	MGI:4828787 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source