EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7228

Npdc1 neural proliferation, differentiation and control gene 1 ( MGI:1099802)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002723. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002723_01	euxassay_002723_02	euxassay_002723_03	euxassay_002723_04

euxassay_002723_05	euxassay_002723_06	euxassay_002723_07	euxassay_002723_08	euxassay_002723_09

euxassay_002723_10	euxassay_002723_11	euxassay_002723_12	euxassay_002723_13	euxassay_002723_14

euxassay_002723_15	euxassay_002723_16	euxassay_002723_17	euxassay_002723_18	euxassay_002723_19

euxassay_002723_20	euxassay_002723_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7228_wholemount_strong.wlz

7228_wholemount_moderate.wlz

7228_wholemount_weak.wlz

7228_wholemount_possible.wlz

7228_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7228_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 moderate expression: see section 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21

telencephalon marginal layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 14 15 16 17 18 19 20 21

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 09 10 11 14 15 16

facial vii ganglion

strong

regional

strong expression: see section 03 04 moderate expression: see section 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 moderate expression: see section 06 07 08 09 17 18 19 20

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 17 18

not examined

regional

not examined expression: see section 02 03 20 21

neural retina

moderate

regional

moderate expression: see section 01 02 03 20 21

axial skeleton

moderate

regional

moderate expression: see section 07 10 11 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5868
Entity Detected:	Npdc1, neural proliferation, differentiation and control gene 1 ( MGI:1099802)
Sequence:	sense strand is shown >T5868 CGCCTCCATCCCATCCCGGATCCATTGCCGCAACGTCAGCGCCACCGCCTCCTCTTTTCCTCTTCCTCCT CCGCCCCTCTCGCTTCCCGGCTTCTCCAGTCGCAGTTCCCGGAGTGGGTACCGAGCCCGGGGCCGTGGGG TGCCATGCTGCCCGGGTAGCGGCGCTGAAGCATGGCGACGCCCGTCCCTCCGCCTTCCCCGCGGCACCTG CGGCTGCTACGGCTGCTGCTCTCCGGCCTCATCCTGGGAGCGGCCCTGAATGGTGCCACCGCCCGCCGCC CGGATGCTACCACCTGTCCTGGAAGCCTGGATTGTGCACTGAAGAGGCGGGCAAAATGCCCACCAGGGGC ACATGCCTGTGGACCATGCCTTCAGTCCTTCCAGGAAGACCAGCGAGGGTTCTGTGTGCCAAGGAAACAC CTATCTTCTGGGGAGGGCCTGCCCCAGCCCAGACTGGAAGAGGAGATCGATTCTCTGGCCCAGGAACTGG CACTGAAGGAGAAGGAGGCGGGGCACTCAAGGCTGACCGCACAGCCCTTGCTTGAGGCTGCGCAGAAGCT CCTGGAGCCTGCAGCTACCCTGGGATTCTCACAGTGGGGTCAACGACTGGAGCCCGGGCTCCCCTCTACT CATGGAACCTCTTCACCTATACCCCATACTTCCTTGAGTTCCCGAGCATCCTCTGGACCTGTGCAGATGT CCCCGCTGGAGCCCCAGGGAAGGCATGGGAATGGCCTCACCCTTGTGCTGATCCTGGCTTTCTGCTTAGC AAGCTCAGCCGCCCTGGCTGTGGCAGCCCTCTGCTGGTGTAGGCTTCAGCGAGAGATCCGCCTGACTCAG AAGGCTGACTATGCTGCCACAGCTAAGGGGCCCACTTCACCTTCAACACCACGAATCTCGCCTGGGGATC AGCGGCTGGCACACAGTGCTGAGATGTATCATTATCAGCACCAGAGACAGCAGATGCTGTGCCTGGAGCG GCATAAGGAACCTCCAAAGGAACTAGAGTCAGCTTCCTCCGATGAGGAAAATGAAGATGGCGACTTCACT GTGTATGAATGCCCAGGCCTGGCGCCAACAGGGGAGATGGAGGTGCGCAACCCACTGTTTGACCATTCCA CGCTGTCAGCACCAGTGCCTGGTCCCCATTCCTTGCCGCCGCTGCAGTGACTCCTAGGCCCACATGCCTG CAGCCCACAGCCCACCAGTGGGGACGAGCGAGTCCTGGTGTTCCCCATGGAAAAGAGCAGCACTGTGACC CCCGCGGGCTTTTGGCAGGAGGGAGGCGCCACCGCTGGAGTTCCTTGCCAGGGAGATTCTCCGCCTTATG TGGTTCTTTTAGCATCTGCTTGCACATTCTCTGTCTGGTATGAACCCTCAAACTGGAGATCCAAGTCCAG GAACCCTAGAGCCCAGGCATGGATAGAGAGTTTTGGAGACAAAAGCCAATTAAAGTTTTTTTCAAACCTG TGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3155595 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3155595 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7229 same embryo
	EMAGE:7230 same embryo
	EMAGE:7226 same embryo
	EMAGE:7225 same embryo
	EMAGE:7227 same embryo
	EurExpress:euxassay_002723 same experiment
	MGI:4826761 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source