EMAGE mouse gene expression spatial database

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EMAGE:7243

Dsc2 desmocollin 2 ( MGI:103221)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004326. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004326_01	euxassay_004326_02	euxassay_004326_03	euxassay_004326_04

euxassay_004326_05	euxassay_004326_06	euxassay_004326_07	euxassay_004326_08	euxassay_004326_09

euxassay_004326_10	euxassay_004326_11	euxassay_004326_12	euxassay_004326_13	euxassay_004326_14

euxassay_004326_15	euxassay_004326_16	euxassay_004326_17	euxassay_004326_18	euxassay_004326_19

euxassay_004326_20	euxassay_004326_21	euxassay_004326_22	euxassay_004326_23	euxassay_004326_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7243_wholemount_strong.wlz

7243_wholemount_moderate.wlz

7243_wholemount_weak.wlz

7243_wholemount_possible.wlz

7243_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7243_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 11 12 13 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 17 18 19 20

epidermis

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 01 02 03 04 05 06

vibrissa

weak

regional

weak expression: see section 04 05 06 07 20 21 22

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 05 06 20 21 22 23 24

cornea epithelium

strong

regional

strong expression: see section 01 02 03 22 23 24

naris

moderate

regional

moderate expression: see section 11 12 13 14 15 16

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 11 14 15 16 17 18 weak expression: see section 07 08 10

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 11 12 14 15 16

esophagus

strong

regional

strong expression: see section 13

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 moderate expression: see section 10

hindgut

moderate

regional

moderate expression: see section 13 14 15 16

rectum

strong

regional

strong expression: see section 09

midgut

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18

midgut loop

moderate

regional

moderate expression: see section 10 11 12 13 14 15 17 18 19

lower jaw incisor

strong

regional

strong expression: see section 11 12 16 17

lower jaw molar

strong

regional

strong expression: see section 07 09 17 18 19

oral epithelium

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

upper jaw incisor

strong

regional

strong expression: see section 11 12 16

upper jaw molar

strong

regional

strong expression: see section 07 19

bladder

weak

regional

weak expression: see section 11 12

kidney calyx

moderate

regional

moderate expression: see section 08 16 17 weak expression: see section 05 06 07

larynx

moderate

regional

moderate expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5114
Entity Detected:	Dsc2, desmocollin 2 ( MGI:103221)
Sequence:	sense strand is shown >T5114 GTGCGGGGCGGTGACCTGTCCCTTGAGCTGGCCATGGCGGCTGTGGGATCTATGCGCTCCGGGAGCCCTG CCTTCGGCCTGGGACACCTGTTGACCCTTGCGATCCTTGCACTTGCCTCTGATGCCTGTAAAGAAGTCGT CCTCCAGGTCCCCTCTGAACTACCTGCCGAGAAATTTGTTGGCAGAGTGAACCTGATGGACTGCCTTAAA TCAGCAGACATAGTTCATCTGAGTGATCCTGACTTCCAAGTCTTAGAAGATGGTTCTGTGTACACAACCA GTTCTGTTGTTTTGTCCTCGGGGCAAAGAAGCTTTACTATATGGCTTTTTAGCACAGACAGCCAAGAAAA AAGGGAGATATCTGTCCATTTAGAGGGCCCAGTAGAGGTACTAAATAAAAGACCGCATACAGAGAAGGTT CTCAGCCGTGCCAAGAGAAGATGGGCTCCTATCCCTTGTTCCATGCTAGAGAATTCATTGGGTCCCTTCC CACTTTTCCTTCAACAGATCCAGTCTGACACAGCTCAAAACTATACCATATACTATTCCATCAGAGGTCC AGGAGTTGATAAAGAACCTCTAAACTTATTTTATGTGGAAAGAGATACTGGAAACCTGTATTGCACTGGT CGTGTAGATCGTGAGCAGTATGAGTCCTTTGAGCTGACTGCATTTGCAACAACTCCAGATGGGTATACGC CAGAGTACCCACTGCCGCTCCTCATTAAAATAGAGGACGAGAACGATAATTACCCGATCTTTACACAGAA ACTGTACAGCTTCACAGTTCAGGAAAATAGTCGCATTGGTTCTATTGTGGGGGAGGTCTGTGCAACCGAC CTGGATGAGCCAGACACAATGCACACTCGCCTGAGGTACTCCATCCTGGAGCAGTCGCCTTCTCCACCCA TGCTATTTACTATGCATCCCAGCACTGGTGTGATCACTACCACGTCGGCTCAGCTGGACAGAGAGTTAAT TGACAAGTACCAGTTGCTAATAAAAGTCCAGGATATGGATGGCCAGTATTTTGGTTTGCACACAACAGCA AAGTGCATCATTACTATTGAAGATGTGAATGACAACCTGCCCACGTTCACTCGCACTACGTATGTGACAT CAGTGGAAGAAAATACAGTTAATGTGGAAATCTTGCGACTTACTGTTCAGGATAAAGATTTAGTTAACTC ACCTAATTGGAGAGCTAATTACACCATTTTGAAGGGCAATGAAAATGGAAATTTTAAAATCGTAACAGAT CCTAAAACCAATGAAGGCATTCTGTGTGTGATTAAGCCTCTGGACTATGAAGAACGGCAACAGGTGACCC TGCAAATTGGAGTAGTTAATGAAGCTCCATACACTAGAGAGGCTAGCTCAAAGTCACCCATGAGCACAGC CACAGTGACTGTCACCGTGACAAATCAGGATGAGGGCCCCGAGTGTATCCCTCCAATGCAAACTGTGAGG ATTCAAGAAAATGTACCAGTTGGGACTAGAAATGATGGATATAAGGCATATGACCCAGAGACCAGAAGCA GCAGTGGCATAAGGTACAGAAAGTTAAGTGACCCAAGAGGGTGGGTCACTGTTAATGAAGATTCAGGATC GATCACTATTTTCCGAGCGTTGGATCGAGAGGCTGAGACTGTCAGAAATGGCATTTACAATATTACAGTC CTTGCGTTAGATGCAGATGGGAGAAGCTGTACTGGAACTCTGGGAATCATACTTGAAGATGTGAATGATA ATGGCCCATTCATACCCAAGCAGACAGTGGTGATATGCAAGGCTACCATGTCCTCTGCTGAAATTGTTGC AGTTGATCTCGATGATCCAGTGAATGGCCCACCATTTGATTTCAGTTTGGAGAGCTCTGATTCGGAAGTT CAGAGGATGTGGAGGCTAACAAGAATCAATGATACAGCAGCTCGTCTTTCCTATCAGAACGACCCTTCAT TTGGATCCTATGCAGTTCCCATTCGAGTTACAGACAGACTTGGTCTGTCCTCAGTAACCACATTGAATGT CCTTGTATGTGACTGCATTACTGAAAGTGACTGCACACTCCGCTCTGGAGAAAGGACTGGCTATGCTGAT GTGAGACTTGGACCATGGGCCATCCTTGCTATACTGTTGGGCATAGCCTTGCTATTTTGTATCCTGTTTA CATTAGTCTGTAGTGTTTCCCGGGCATCAAAACAACAGAAAATTCTTCCTGATGACTTAGCTCAACAGAA CCTAATTGTATCAAACACCGAGGCTCCTGGAGATGACAAAGTTTATTCCACAAACGGCTTGACAACTCAA ACTATGGGAGCTTCTGGGCAGACAGCTTTCACCACCATGGGAACTGGAGTCAAAAGTGGAGGGCAAGAGA CCATTGAGATGGTAAAAGGAGGGCAGCAGACTTTGGACTCACGCAGAGGGGCTGGCTATCATCACCATAC CCTGGATCCTTGCAGGGGAGGACATGTGGAGGTGGACAACTACAGACACACTTACTCAGAGTGGTACAAT TTCATTCAGCCCCGACTTGGTGATGAAACCATTAGAGGACACACTCTGATTAAAAATTAAACAATAAAAG AAGGTACAGTTTTGCCATACGGATGACAACCAGAAGCTTGCTCAAGACTACGTCCTCACATACAACTATG AGGGAAAAGGCTCGGCTGCTGGCTCTGTGGGTTGCTGTAGTGACCTACAGGAAGAAGACGGGCTTGAGTT TTTGGACCATCTGGAGCCCAAATTTAGGACATTGGCAGAAGTATGTGCAAAGAGGTGAACGACTTCTAAT AGGAAAACGAAAACTAATGGATTCACTGCCTTGCCCTCCCTGCTTTTCACTGTAAACCAGGAGTTTTCAA AAAACAGAAGATGTTATATTTGGGGCTCCCTCTCCTATCTCGGTCTGCAGAATCTCCATGCAAATGCACA TTTACAGAGAAACTTTGCAATTAGTACTTGAAAACCATTATGCATGGATTTCAATTACTTCTTTTTTACC AGATGACATCTTAGGTGGAAACTTAAGGAAATTTGCATTACTGTTTACATTGGGTATAATGACAGCAGCC AACTTATAGTGCAATAAAGTTGAATGGATTTGAGTCTATTATAGACTATTGACTATAACCCAATTGGAGA CTGTAGACACCATTATCTCCAGTTAATGGATTAACTGGTTGTTCATACGGTGCTTGGAAACAGTTGTTTT CCTAAACATTCTAAACTGTATATGCTGTGCCTTGCTTTAGTTATATTCTTGTAATATGGCCTGGCTTCAT AGTACAAAGGTAGATTGCCAGGCAGCCTTTGAGTGTGCTTGAAATTCCAATCCAGTGGGTGTTAGCTTCT GTTCTGTGTAAAATCCTGCCCTGAATCACAATCTCTTTTGTACCAACTTTGTCAGTTAAATTTAGTTTTC AAAACTTCTTTATAATGTGTTCTGTTTTTCTAAAAGTTTTGTCTGTGAACACCAAATGCTCTAAACTCCC ATCTTCTCTATTTTTAATTTTTTTTTCATTTTCTCTCCTGTTTGACTTTGCAAGGTCTGCTTCCAAGATA TTCCTAAAACTTATCTTTTCTCATAAAATAGTTGGTGTGTATGCATATCATTGTTGGTTTTCTTAAGCTC AATATAATAGCAACATTATTGTTTTGGATTTTTTGGTAATTATTGCTTCATTTTTGTCCTTAGTAAAGAA CTTTTAATTTTCCCAGTGAAACTATGGGACTCTTTCATAATCTTTTTGAAGCAATGTTCTTCGTGACAGA AGCAATAGTGTATTTAGAAGTAGGTTGTGGCAATCTAATATAGAATAGCATAGTTACAATTTCCATCTTT AATTCAGGGACCCTGGAAGTGGGAAGTAGTGATAGAGTTTAGACTAAGCATCTCAGCATAATAATCTCAA ACAATCTCTAGGATAATGCCTTCTCCAGTGTTTTAGATGATATGGCTAGTTATAATTGCATTGTTGTAAA ATTAGTTTTAGAAGCAGTTTGTAATTATAGTTATTTTATGAAATATAGGAAATGGGCCAAGCAATTTAAA ATTTTTTAGAAATTTACACACAAGTAAACTTCATACCACAGGACACACTCCCTTCAATCAGTGAGCTCAG CACTTAACCTAGATGGATGGATATTCTAGGTTGGATAATTGGTGCAATATAGATATGTGTGATTGTTCAC TGTGTGTCTGTCTTGAATTTATCAAGTTGGCTTACAACAATTGTTTTCAGAACAAACATTGGGAAAAAAT GTAAACATGAACTATGTATGCAGTGAGGTCATTATTACTTAGTGAAAAATATTTCTCAGAGTTATTTTTA ATAAATTATATATAAAATGGTAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5327748 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5327748 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7244 same embryo
	EMAGE:7247 same embryo
	EMAGE:7246 same embryo
	EMAGE:7242 same embryo
	EMAGE:7245 same embryo
	EurExpress:euxassay_004326 same experiment
	MGI:4824396 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source