EMAGE mouse gene expression spatial database

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EMAGE:7247

Olfml2b olfactomedin-like 2B ( MGI:2443310)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004338. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004338_01	euxassay_004338_02	euxassay_004338_03	euxassay_004338_04

euxassay_004338_05	euxassay_004338_06	euxassay_004338_07	euxassay_004338_08	euxassay_004338_09

euxassay_004338_10	euxassay_004338_11	euxassay_004338_12	euxassay_004338_13	euxassay_004338_14

euxassay_004338_15	euxassay_004338_16	euxassay_004338_17	euxassay_004338_18	euxassay_004338_19

euxassay_004338_20	euxassay_004338_21	euxassay_004338_22	euxassay_004338_23	euxassay_004338_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7247_wholemount_strong.wlz

7247_wholemount_moderate.wlz

7247_wholemount_weak.wlz

7247_wholemount_possible.wlz

7247_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7247_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

moderate

regional

moderate expression: see section 01 02 03 04 21 22 23 24

hindlimb digit 1 metatarsal

moderate

regional

moderate expression: see section 03 17 18

hindlimb digit 2 metatarsal

moderate

regional

moderate expression: see section 03 04 05 17 18 19

hindlimb digit 3 metatarsal

moderate

regional

moderate expression: see section 03 04 05 17 18 19

hindlimb digit 4 metatarsal

moderate

regional

moderate expression: see section 03 04 05 17 18 19

hindlimb digit 5 metatarsal

moderate

regional

moderate expression: see section 04 05

foot mesenchyme

moderate

regional

moderate expression: see section 02 04 05 15 16 18 19

knee rest of mesenchyme

moderate

regional

moderate expression: see section 23 24

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 01 19 20 21 22 weak expression: see section 18

femur

weak

regional

weak expression: see section 01 15 16 17 18 19

upper leg rest of mesenchyme

moderate

regional

moderate expression: see section 01 02 17 19 20 weak expression: see section 18

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

cerebral cortex mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21

trigeminal v nerve mandibular division

moderate

regional

moderate expression: see section 04 05 06 07 18 19 20

trigeminal v nerve maxillary division

moderate

regional

moderate expression: see section 05 06 07 08 18 19 20

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

neural retina

weak

regional

weak expression: see section 01 02 23

esophagus

weak

regional

weak expression: see section 12 13

tongue muscle

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 weak expression: see section 10

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 10 11 15 16

axial skeleton

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15

nucleus pulposus

strong

regional

strong expression: see section 08 10 11 12 13 14

clavicle

moderate

regional

moderate expression: see section 08 09 10 11 15 16 17 weak expression: see section 18

scapula

moderate

regional

moderate expression: see section 01 02 03 04 22 23 24

pelvic girdle skeleton

moderate

regional

moderate expression: see section 04 05 06 13 14 15

tail mesenchyme

moderate

regional

moderate expression: see section 08 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5135
Entity Detected:	Olfml2b, olfactomedin-like 2B ( MGI:2443310)
Sequence:	sense strand is shown >T5135 CCACGCGTCCGAGTGAAGCCGCCTTCCAGCCTGTCTTTGCTGAGACCTCCGACCCAAGGTGGTCTCTGTA GGGACTAAAGTCCCTACTGTCGCATCTCTCATGGCCTATCCCCTGCCATTGGTTCTCTGCTTTGCTCTGG TGGTGGCACAGGTCTGGGGGTCCACTACACCTCCCACAGGGACAAGCGAGCCCCCTGATGTGCAAACAGT GGAGCCCACGGAAGATGACATTCTGCAAAACGAGGCGGACAACCAGGAGAACGTTTTATCTCAGCTGCTG GGAGACTATGACAAGGTCAAGGCTGTGTCTGAGGGCTCTGACTGTCAGTGCAAATGTGTGGTGAGACCGC TGGGCCGAGATGCCTGCCAGAGGATCAACCAGGGGGCTTCCAGGAAGGAAGACTTCTACACTGTGGAAAC CATCACCTCGGGCTCATCCTGTAAATGTGCTTGTGTTGCTCCTCCGTCTGCCGTCAATCCCTGTGAGGGA GACTTCAGGCTCCAGAAGCTTCGGGAGGCTGACAGCCGAGATTTGAAGCTGTCTACAATTATAGACATGT TGGAAGGTGCTTTCTACGGCCTGGACCTCCTAAAGCTGCATTCGGTTACCACTAAACTCGTGGGGCGAGT GGATAAACTGGAGGAGGAAGTCTCTAAGAACCTCACCAAGGAGAATGAGCAAATCAAAGAGGACGTGGAA GAAATCCGAACGGAGCTGAACAAGCGAGGCAAGGAGAACTGCTCTGACAACACCCTAGAGAGCATGCCAG ACATCCGCTCAGCCCTGCAGAGGGATGCGGCTGCAGCCTACGCCCACCCAGAGTATGAAGAACGGTTTCT GCAGGAGGAAACTGTGTCACAGCAGATCAACTCCATCGAACTCCTGAGGACGCAGCCACTGGTCCCTCCT GCAGCGATGAAGCCGCAGCGGCCCCTGCAGAGACAGGTGCACCTGAGAGGTCGGCTGGCCTCCAAGCCCA CCGTCATCAGGGGAATCACCTACTATAAAGCCAAGGTCTCTGAGGAGGAAAATGACATAGAAGAGCAGCA CGATGAGCTTTTCAGTGGCGACAGTGGAGTGGACTTGCTGATAGAAGATCAGCTTCTAAGACAGGAAGAC CTACTGACAAGTGCCACCCGGAGGCCAGCAACCACTCGTCACACTGCTGCTGTCACGACTGATGCGAGCA TTCAGGCCGCAGCCTCATCCTCAGAGCCTGCACAGGCCTCTGCCTCAGCATCCAGCTTTGTTGAGCCTGC TCCTCAGGCCTCCGATAGAGAGCTCTTGGCAACCCCACAGACTACCACAGTGTTTCCAGAGCCCACGGGG GTGATGCCTTCTACCCAAGTCTCACCCACCACCGTGGCCCACACAGCTGTCCAGCCACTTCCAGCAATGG TTCCTGGGGACATATTTGTGGAAGCTCTACCCTTGGTCCCTCTGTTACCTGACACAGTTGGGACAGACAT GCCAGAGGAAGAGGGGACTGCAGGGCAGGAAGCAACCTCTGCTGGTCCCATCCTGAGCCCTGAAGAAGAA GATGACATTCGGAATGTGATAGGAAGGTGCAAGGACACCCTCTCTACAATCACAGGACCGACCACCCAGA ACACATATGGACGGAATGAAGGGGCCTGGATGAAGGACCCCCTAGCCAAGGACGACCGCATTTACGTAAC CAACTATTACTATGGCAACACACTGGTCGAGTTCCGAAACCTGGAGAACTTCAAACAAGGTCGCTGGAGC AATTCCTACAAGCTTCCATACAGCTGGATCGGCACGGGTCACGTGGTCTACAACGGCGCCTTCTACTATA ACCGGGCCTTCACCCGAAACATCATCAAGTATGACCTGAAGCAGCGTTATGTGGCTGCCTGGGCCATGCT GCACGATGTGGCCTATGAGGAGGCCACTCCTTGGCGGTGGCAGGGTCACTCGGATGTGGACTTTGCTGTG GATGAGAATGGCCTGTGGCTTATCTACCCAGCTCTGGATGATGAAGGTTTCAACCAGGAGGTCATTGTCC TGAGCAAGCTCAATGCCGTGGACCTGAGCACGCAGAAGGAGACCACGTGGCGCACTGGGCTCCGGAGGAA TTTCTATGGCAACTGCTTTGTCATCTGTGGGGTACTATATGCTGTGGACAGCTATAACCAGAGGAATGCC AACATCTCCTATGCCTTTGACACACACACCAACACACAGATTGTCCCTAGGCTGCTGTTTGAGAATGAAT ATTCGTACACCACCCAGATAGACTACAACCCCAAGGACCGCCTCCTCTATGCCTGGGACAATGGCCACCA GGTCACCTACCATGTCATCTTTGCCTACTGACACACTTGACCCTGCAAAAAGAAGCACAGTGGGGCCACT AGCACCTTGTGTGTGTCTGTGTGCATGTCTGTCTGTGAGATTGTGCAGGTGGGTGTGTGTTGTTTTAAAA TATATTATTTTGTATAATATTACAAGTGTAAAATGACAGTTTGGGTCTATTTTTTTTATATGGATTGTAG ATCAATCCATATGTGTATGTGCTGGTCTCATCCTTCACAATTTATATTTTTGTGCAAATGAACTTCTCCT TCTGACCAGTAACTACCTTCTTTCGTGCTCTGAACCTCTGGCTCCTGAGGTCAAGGGCTGGAGGGTTTCT TCCTCCAGGTCTTGCAGCCAGGAGCAGGAGTGTGGGGCTCAGGAAAAAGTGCTAAGTGGCGGCAAAGTTT TTATGTATTAGAGAAGTTCTTAAAACTCAGAAAAAAATACTTTTTTTAAATAAAGGAGATATTTTAAGAC CCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5342879 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5342879 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7244 same embryo
	EMAGE:7243 same embryo
	EMAGE:7246 same embryo
	EMAGE:7242 same embryo
	EMAGE:7245 same embryo
	EurExpress:euxassay_004338 same experiment
	MGI:4826856 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source