EMAGE mouse gene expression spatial database

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EMAGE:7276

Eln elastin ( MGI:95317)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004329. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004329_01	euxassay_004329_02	euxassay_004329_03	euxassay_004329_04

euxassay_004329_05	euxassay_004329_06	euxassay_004329_07	euxassay_004329_08	euxassay_004329_09

euxassay_004329_10	euxassay_004329_11	euxassay_004329_12	euxassay_004329_13	euxassay_004329_14

euxassay_004329_15	euxassay_004329_16	euxassay_004329_17	euxassay_004329_18	euxassay_004329_19

euxassay_004329_20	euxassay_004329_21	euxassay_004329_22	euxassay_004329_23	euxassay_004329_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7276_wholemount_strong.wlz

7276_wholemount_moderate.wlz

7276_wholemount_weak.wlz

7276_wholemount_possible.wlz

7276_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7276_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 14 15 16 17 18 19 20 21 weak expression: see section 22

humerus

strong

regional

strong expression: see section 01 02 23 24 moderate expression: see section 03 21 22

hindlimb digit 1 mesenchyme

weak

regional

weak expression: see section 02 19

hindlimb digit 2 mesenchyme

weak

regional

weak expression: see section 02 03 18 19

hindlimb digit 3 mesenchyme

weak

regional

weak expression: see section 02 03 18 19

hindlimb digit 4 mesenchyme

weak

regional

weak expression: see section 02 03 18 19

hindlimb digit 5 mesenchyme

weak

regional

weak expression: see section 02 18 19

foot mesenchyme

moderate

regional

moderate expression: see section 02 weak expression: see section 18 19

knee rest of mesenchyme

moderate

regional

moderate expression: see section 01 weak expression: see section 20

fibula

weak

regional

weak expression: see section 22 23 24

tibia

weak

regional

weak expression: see section 22 23 24

femur

strong

regional

strong expression: see section 01 02 03 17 18 19 20

physiological umbilical hernia

strong

regional

strong expression: see section 11 12

vibrissa

weak

regional

weak expression: see section 03 04 05 06 20 21 22

telencephalon ventricular layer

weak

regional

weak expression: see section 05 06 07 08 09 10 15 16 17 18 19

trigeminal v nerve maxillary division

moderate

regional

moderate expression: see section 05 06 07 19 20

extrinsic ocular muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 06 19 20 21 22 23 24

nasal septum

moderate

regional

moderate expression: see section 12 13

viscerocranium

moderate

regional

Expression in the turbinate bone.

aorta

strong

regional

strong expression: see section 09 10 11 12 13 14

heart valve

moderate

regional

moderate expression: see section 10 11 12 13

tongue muscle

strong

regional

strong expression: see section 13 moderate expression: see section 11 12 14 15 weak expression: see section 10

lower lip

strong

regional

strong expression: see section 13 14 15 16 moderate expression: see section 06 07 09 10 11 12 17 18 19

mandible

moderate

regional

moderate expression: see section 21 22 weak expression: see section 02 03 04 05 06 07 08 09 15 16 17 18 19 20

upper lip

strong

regional

strong expression: see section 13 14 15 16 moderate expression: see section 08 09 10 11 12 17 18 19

palatal shelf

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16

lung

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 13 14 15 16 17 18 19 20 21 22

trachea

moderate

regional

moderate expression: see section 13 weak expression: see section 12

exoccipital bone

strong

regional

strong expression: see section 02 03 moderate expression: see section 05 21

vault of skull

strong

regional

strong expression: see section 02 03 23 24 moderate expression: see section 04 20 21 22

orbito-sphenoid

strong

regional

strong expression: see section 02 03 23 24 moderate expression: see section 04 05 06 07 20 21 22

scapula

moderate

regional

moderate expression: see section 01 02 03 20 21 22 23

pelvic girdle skeleton

strong

regional

strong expression: see section 15 moderate expression: see section 05 06 07 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5162
Entity Detected:	Eln, elastin ( MGI:95317)
Sequence:	sense strand is shown >T5162 CTCACAGCAGCCTACCAGGCAGCAATTACGCTTTGGGGATAAAACGAGGCGCTGAGAGCAGGCTGGGGCA GTCCTCCCCGAGATGGCGGGTCTGACAGCGGTAGTCCCGCAGCCTGGCGTCTTGCTGATCCTCTTGCTCA ACCTCCTCCATCCCGCGCAGCCTGGAGGGGTTCCAGGAGCTGTGCCTGGCGGACTTCCTGGTGGAGTTCC CGGTGGAGTCTATTATCCAGGGGCTGGTATTGGAGGCCTGGGAGGAGGAGGAGGAGCTCTGGGACCTGGA GGAAAACCACCTAAGCCAGGTGCCGGACTTCTGGGAACGTTTGGAGCAGGTCCTGGAGGACTTGGAGGTG CTGGCCCGGGTGCAGGTCTCGGGGCCTTTCCTGCAGGCACCTTCCCAGGGGCAGGAGCTCTGGTGCCCGG GGGAGCAGCAGGGGCTGCTGCGGCTTATAAAGCTGCCGCCAAAGCTGGGGCTGGGCTTGGTGGCGTTGGC GGAGTCCCAGGTGGTGTTGGCGTTGGTGGAGTTCCAGGTGGTGTTGGAGTTGGCGGAGTCCCAGGTGGTG TTGGAGTTGGTGGAGTCCCTGGCGGTGTTGGTGGTATTGGTGGCATCGGTGGCTTAGGAGTCTCGACAGG TGCTGTGGTGCCCCAAGTCGGAGCTGGCATCGGAGCTGGAGGAAAGCCTGGGAAAGTTCCTGGTGTTGGT CTTCCAGGTGTATACCCAGGCGGAGTGCTCCCAGGAACAGGAGCTCGGTTCCCTGGTGTGGGGGTGCTCC CTGGAGTTCCCACTGGCACAGGAGTCAAAGCCAAGGCTCCAGGTGGAGGTGGTGCTTTTGCTGGAATCCC AGGGGTCGGACCCTTTGGGGGTCAGCAGCCTGGTGTCCCACTGGGTTATCCCATCAAAGCACCAAAGCTG CCAGGTGGCTACGGACTGCCCTATACCAATGGGAAATTGCCCTATGGAGTAGCTGGTGCAGGGGGCAAGG CTGGCTACCCAACAGGGACAGGGGTCGGATCCCAGGCGGCGGCGGCAGCAGCTAAAGCAGCCAAGTATGG TGCTGGGGGAGCTGGAGTCCTCCCTGGTGTTGGAGGGGGTGGCATTCCTGGTGGTGCTGGCGCAATTCCT GGAATTGGAGGCATTGCAGGCGCTGGAACTCCTGCAGCAGCAGCTGCTGCAAAGGCTGCTGCTAAGGCTG CTAAGTATGGAGCTGCTGGAGGTTTAGTGCCTGGTGGACCAGGAGTTAGGCTCCCAGGTGCTGGAATCCC AGGTGTTGGTGGCATTCCTGGTGTTGGTGGCATCCCAGGTGTTGGGGGCCCTGGTATTGGAGGTCCAGGC ATTGTGGGTGGACCAGGAGCTGTGTCACCAGCTGCAGCTGCTAAAGCTGCTGCCAAAGCTGCCAAATACG GAGCCAGAGGTGGAGTTGGCATCCCGACATATGGGGTTGGTGCTGGTGGCTTTCCTGGCTATGGTGTTGG AGCTGGAGCAGGACTTGGAGGTGCAAGCCCAGCTGCTGCTGCTGCCGCCGCCAAAGCTGCTAAGTATGGT GCTGGAGGAGCTGGAGCCCTGGGAGGCCTGGTGCCAGGTGCAGTACCAGGTGCACTGCCAGGTGCAGTAC CAGCTGTGCCGGGAGCTGGTGGAGTGCCAGGAGCAGGTACCCCTGCAGCTGCAGCTGCTGCCGCCGCCGC TAAAGCAGCCGCCAAAGCAGGTTTGGGTCCTGGTGTTGGTGGGGTTCCTGGTGGAGTTGGTGTTGGTGGG ATTCCCGGTGGAGTTGGTGTTGGTGGGGTTCCTGGTGGAGTTGGCCCTGGTGGTGTTACTGGTATTGGAG CTGGTCCTGGCGGTCTTGGAGGAGCAGGGTCACCGGCTGCCGCTAAATCTGCTGCTAAGGCAGCTGCCAA AGCCCAGTACAGAGCTGCCGCTGGGCTTGGAGCTGGTGTCCCTGGATTTGGGGCTGGTGCTGGTGTCCCC GGATTTGGGGCTGGTGCTGGTGTCCCCGGATTTGGGGCTGGTGCTGGTGTCCCCGGATTTGGGGCTGGTG CTGGTGTCCCTGGATTTGGAGCTGGAGCAGTACCTGGATCGCTGGCTGCATCCAAAGCTGCTAAATATGG AGCAGCAGGTGGCCTTGGTGGCCCTGGAGGTCTCGGTGGCCCTGGAGGTCTCGGTGGACCTGGAGGACTT GGTGGGGCTGGTGTTCCCGGTAGAGTAGCAGGAGCTGCACCCCCTGCTGCTGCCGCTGCTGCTGCCAAAG CTGCTGCTAAGGCTGCCCAGTATGGCCTTGGTGGAGCCGGAGGATTGGGAGCCGGTGGACTGGGGGCCGG TGGACTGGGAGCCGGTGGACTGGGAGCTGGTGGACTGGGAGCCGGTGGACTGGGAGCTGGTGGACTGGGA GCCGGTGGACTGGGAGCTGGTGGAGGTGTGTCCCCTGCTGCAGCTGCTAAGGCAGCCAAATATGGTGCTG CTGGCCTTGGAGGTGTCCTAGGAGCCAGGCCATTCCCAGGTGGAGGAGTTGCAGCAAGACCTGGCTTTGG ACTTTCTCCCATTTATCCAGGTGGTGGTGCTGGGGGCCTGGGAGTTGGTGGAAAACCCCCGAAGCCCTAT GGAGGAGCCCTTGGAGCCCTGGGATACCAAGGTGGGGGCTGCTTTGGGAAATCCTGTGGCCGGAAGAGAA AGTGATCTTCTGGGGACCCCTGACTCGCGACCTCATCAACGTTGGTGCTACTGCTTGGTGGAGAATGTAA ACCTTCTATGACCACCCCCTCCTCCATCCCCCTGACCCCCACCTGGGAGGGGACAACAGGCCAGTGGCCT TGGAAACCCACAGGACAAGGAAATCAGACAGCAGCAGCCATGCAGCCCTAACCAGAAACTCCCCCCACCC TATATCAGAGGCCAGGGCGGGTGTCCCATCTCTTCCCACCCAGGAGCTCCCCCCCACAGTCTCCATCTCC AAGGGAAATTGGTGCTACATGTTGGTGCTTCTTCTTTGTGGGGGGAGGGAGGAGGGAAGGGTATCCCAGG GGGGATTGCCCCCTTCCCTGAAGCCCCTCTATTAAGATGGTGCACACCTTTGTTGGGCAGTCCCACCTCC CCCTGCCCACCAGGAGCCATTCCTGGCTGCATCCCATTGGTACCCAAATACCGGAAGCCTTGACGATGGA TTTGGTGACATGATCCCTCTCTCTTTGGTTCCCCTGTCCCTGCCTCCTGTTACCTAAAGCTACTTCCCAC ATCTGGGACACCCTGGAGTCAGATGGCTCCTCACACTGGGAATAGCTCCCTTGTTCTTATGGAATCCACC TGCCATCCACCCATCCACCTACTCATCCATCCATCCATCCATCCATCCATCCGTCCATCTTGACTGCCTA GTACCACTAAGCTGGCTGGGCATACCCACTATCAACCTGGTTCACCTGTCATGGCAGCCTGTCCCCGTCC CCACCACACACCCCGATCCTGGCCTAGGGTGCAAAGGGTTGTGTGGGCTGGTTGTCCCCACATGCAGTAC TGTAACCCCGTTCTTCCTGGAGCCACTCTTACAGAGCATGTCTCACCACCCCACCTCTTTGTGTTTCGCT GTGATAGATCAATAAAATATTTTATTTTTTGTCCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:5346255 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5346255 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7280 same embryo
	EMAGE:7277 same embryo
	EMAGE:7279 same embryo
	EMAGE:7275 same embryo
	EMAGE:7278 same embryo
	EurExpress:euxassay_004329 same experiment
	MGI:4824531 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source