EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7278

Mboat1 membrane bound O-acyltransferase domain containing 1 ( MGI:2387184)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004364. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004364_01	euxassay_004364_02	euxassay_004364_03	euxassay_004364_04

euxassay_004364_05	euxassay_004364_06	euxassay_004364_07	euxassay_004364_08	euxassay_004364_09

euxassay_004364_10	euxassay_004364_11	euxassay_004364_12	euxassay_004364_13	euxassay_004364_14

euxassay_004364_15	euxassay_004364_16	euxassay_004364_17	euxassay_004364_18	euxassay_004364_19

euxassay_004364_20	euxassay_004364_21	euxassay_004364_22	euxassay_004364_23	euxassay_004364_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7278_wholemount_strong.wlz

7278_wholemount_moderate.wlz

7278_wholemount_weak.wlz

7278_wholemount_possible.wlz

7278_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7278_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

moderate

regional

moderate expression: see section 05 06 07 08 09 13 14 15

vibrissa

strong

regional

strong expression: see section 05 06 07 19 20

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12

rest of cerebellum ventricular layer

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 not examined expression: see section 12

midbrain ventricular layer

strong

regional

strong expression: see section 11 12 13

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 14 15 17 18 weak expression: see section 07 08 16

esophagus

moderate

regional

moderate expression: see section 11 weak expression: see section 12

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

hindgut

moderate

regional

moderate expression: see section 11 12 13 14 15 16

rectum

moderate

regional

moderate expression: see section 10

midgut

moderate

regional

moderate expression: see section 11 12 13 14 15 16

midgut loop

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

bladder

moderate

regional

Expression in the fundus region.

kidney calyx

moderate

regional

moderate expression: see section 18 weak expression: see section 04 05 06 07 08 14 15 16 17

male reproductive system

weak

regional

weak expression: see section 11

urethra of male

weak

regional

weak expression: see section 10 11

trachea

weak

regional

weak expression: see section 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5163
Entity Detected:	Mboat1, membrane bound O-acyltransferase domain containing 1 ( MGI:2387184)
Sequence:	sense strand is shown >T5163 CCACGCGTCCGGCGGTGCGGAGCGGGAAGCAGAACGTGGGGGCCCTGCCCTCCCAGGCGCGGGGTCCCGC AGCGCCAGGTCCGCGCCCTGGAGTCGGCGCCAGGTTCCCGCGAGGTCCTCAGGAGCCCTCAAGGTGGCCA GTGTGCAGCTCCCGGGCGACAGAGCACCCTGAAGATGCAGGATGGCAGCACGGCCGCCCGCCAGCCTCTC TTACCGTACCACCGGCTCCACCTGCCTGCACCCGCTCAGCCAGCTCCTGGGCATCCCGCTGGATCAGGTT AACTTTGTGGCTTGCCAGCTCTTTGCCTTGTCTGCTGCTTTCTGGTTCAGAATCTACTTACATCCTGGTA AAGCCAGCCCTGAGGTCCGGCACACCTTGGCCACCATTTTGGGCATCTATTTTGTTGTGTTTTGTTTTGG TTGGTATGCTGTACATCTCTTTGTGCTGGTGTTGATGTGTTATGGGGTCATGGTCACTGCAAGTGTATCC AATATTCACAGGTATTCCTTTTTTGTAGCCATGGGCTACCTTACGATATGCCACATCAGCCGCATTTACA TCTTCCACTATGGAATTCTCACTACAGATTTTTCTGGGCCCCTGATGATTGTCACTCAGAAGATCACGAC GTTGGCTTTCCAAGTTCATGATGGATTGGGTCGAAAAGCTGAAGACCTTTCTGCTGAGCAACACCGACTT GCTGTGAAAGCGAAGCCCTCGCTTCTGGAATACTTAAGCTACCATCTCAACTTTATGAGTGTCATAGCCG GCCCTTGCAACAATTTCAAGGACTACGTAGCCTTCATCGAAGGGAGACATATACACATGAAGTTGCTGGA AGTGAACTGGACGCAAAGGGGTTTCCAGAGTTTGCCAGAGCCTTCTCCCATGGGAGCTGTGATACAGAAG TTGTGTGTGACCTTGATGTCTCTCCTGTTGTTTTTGACGCTCTCCAAGTCCTTTCCCGTCACCTTCCTTA TTGATGACTGGTTTGTACATAAGGCCAACTTTCTGAGTCGTCTCTGGTACTTATATGTCGTCATGCAAGC CGCAAAGCCCAAGTATTACTTTGCGTGGACATTAGCAGATGCGGTGCACAATGCAGCTGGATTCGGGTTC AATGGCATGGACACGGATGGGAAGTCTCGCTGGGATTTACTATCTAACCTGAACATCTGGAAGATTGAGA CTGCCACGAGTTTCAAAATGTACTTGGAAAACTGGAATATTCAGACATCTACGTGGCTGAAATGTGTGTG CTATGAGCGGGTTCCCTGGTACCCCACAGTGCTCACCTTCCTCCTGTCTGCTCTGTGGCACGGCGTCTAC CCTGGATACTACTTCACATTCCTAACTGGAGTCCCTGTCACATTGGCAGCCAGAGCGGTGAGGAACAACT ACAGACACCACTTCCTCTCTTCCAAAGCTCGAAAGATTGCCTATGACGTGGTGACCTGGGCTGTCACTCA GTTGGCTGTCTCTTACACGGCAGCGCCTTTCGTCATGTTGGCAGTCGAGCCAACCATCAGTTTATACAAG TCCGTGTTCTTTTTTTTACACATCATATGTCTGCTGATAATCCTCTTTCTGCCAATCAAACCACACCAGC CTCAAAGGCAGTCTCGGAGTCCAAATTCTGTAAAGAAGAAGGCAGACTGATACCCACAGAGAAACGGTGA ACAAGAGAGTTGCGGGACATTGGGAAGACATAACGGACAGGCTCAAGGGTTCTCCAGTGACTCGGAGGAG ATGTTTACATGTATTTTTTTTTTTCCGCTGCAGGGAATATTTTTATGTCTTTTTTTTTTTTTGTACCATT AAATCACCATGTCTAGCTTTGTCTGCATACTTTACAGGGCCTTTCAGAAACACAGCATAGACAAGAGGCC ACTGGGTAGGCTCTTCTAAAATCATTTGCACCTACTGAAATCTGGAAGCATGACTCCAGCCGTTGCCTCC GGCACTTAGGAGGTGTCTGCTGAGATGACTTGTCTGGAGTTTTGAAATCGGAACATTCCCAGGCATGATT TTTTTTTCCTCTTGGCAACTCCAGACCCTGGGATATGCAACTTAGTAGGCTGTGGACACAACTCTTATGC TCAAAAGCCCTTGGAGGAAAGAGATTGTGAAGGGAAGTTTTCAGGGTATAAAAAAAAAAAGAAAAACCTC CAGGAACTGGGAAGATTAGCCCCTGAATGCTTGAATGGATCGATGCGTCCCCAAACCAACTGCATTATCA CCCCAACTCAGCCAGGAATGTACCATTCCCTTTTTTCCTTCTGAATTGAGCCAAGATTGGACATCCGAAA GCTGCCTGCGTGCCCCAGGAGTTGGGGAGAGAGTGGGGGCTACTGGTTTCCATTTCCCGTGCTTGGATCT GATTGACTTCTATTGACCCTGAGAAGGCACCCGGATACCAACCAGCACACCGTCCAAAGGCGGCCAAATG TCTTTTCAAGAAATAGCTGCATTCAGCATGCAGAGGCTCCCTTTTGTCTCTGCATATCAAGGAAGTGTCG ATGATCACGGCCAACGTGTAGACACTAGGTAACGTCTGTAACGGGCTACAGCCAGCTTTATTTTAGGTGA TCTGTCATGTCGGAAAGCTGCAGGGGAACAACAGTGCTCTACTCTGTCTTTGTTATGAATGTGCTGTGGC TCCTTCGGGGAATTTCTACTGGATAAAATAGTCTATGTGAAAGAAGGAAACATAATATTTAAAATGTGGA CGATAATAATAATGATGATGATAATAATAATAATAAAATATGCGCTTCAATGATGTGCCTCAATCAAATG GCCTGAAAGGTGAAAATTTGTTCTGAACAGATGATTTTTAAAAAATTAGTTTCTCACTGAATAAATAATA TATTTCTATTTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5346000 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5346000 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7280 same embryo
	EMAGE:7277 same embryo
	EMAGE:7276 same embryo
	EMAGE:7279 same embryo
	EMAGE:7275 same embryo
	EurExpress:euxassay_004364 same experiment
	MGI:4826119 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source