EMAGE mouse gene expression spatial database

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EMAGE:7331

Lpl lipoprotein lipase ( MGI:96820)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004410. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004410_01	euxassay_004410_02	euxassay_004410_03	euxassay_004410_04

euxassay_004410_05	euxassay_004410_06	euxassay_004410_07	euxassay_004410_08	euxassay_004410_09

euxassay_004410_10	euxassay_004410_11	euxassay_004410_12	euxassay_004410_13	euxassay_004410_14

euxassay_004410_15	euxassay_004410_16	euxassay_004410_17	euxassay_004410_18	euxassay_004410_19

euxassay_004410_20	euxassay_004410_21	euxassay_004410_22	euxassay_004410_23	euxassay_004410_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7331_wholemount_strong.wlz

7331_wholemount_moderate.wlz

7331_wholemount_weak.wlz

7331_wholemount_possible.wlz

7331_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7331_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

spotted

strong expression: see section 01

forearm rest of mesenchyme

strong

spotted

strong expression: see section 01

upper arm rest of mesenchyme

strong

spotted

strong expression: see section 02 22 23 24

interdigital region between forelimb digits 1 and 2

strong

spotted

strong expression: see section 01 24 moderate expression: see section 02 03 04 23

interdigital region between forelimb digits 2 and 3

strong

spotted

strong expression: see section 01 24 moderate expression: see section 02 03 04 23

interdigital region between forelimb digits 3 and 4

strong

spotted

strong expression: see section 01 24 moderate expression: see section 02 03 04 23

interdigital region between forelimb digits 4 and 5

strong

spotted

strong expression: see section 01 24 moderate expression: see section 02 03 04 23

interdigital region between hindlimb digits 1 and 2

strong

spotted

strong expression: see section 06 07 08 09 20 21 22 weak expression: see section 10 11

interdigital region between hindlimb digits 2 and 3

strong

spotted

strong expression: see section 06 07 08 09 20 21 22 weak expression: see section 10 11

interdigital region between hindlimb digits 3 and 4

strong

spotted

strong expression: see section 06 07 08 09 20 21 22 weak expression: see section 10 11

interdigital region between hindlimb digits 4 and 5

strong

spotted

strong expression: see section 06 07 08 09 20 21 22 weak expression: see section 10 11

lower leg rest of mesenchyme

strong

spotted

strong expression: see section 01 02 03 04 05 22 23 24

upper leg rest of mesenchyme

strong

spotted

strong expression: see section 02 03 04 05 06 07 08 20 21 22 23 24

mesenchyme

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pectoral girdle and thoracic body wall muscle

strong

regional

strong expression: see section 02 03 04 05 06 07 08 19 20 21 22 23 24

submandibular gland primordium mesenchyme

strong

regional

strong expression: see section 07 08 09 10 18 19 20

cerebral cortex marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

metencephalon part of 4th ventricle choroid plexus

strong

spotted

strong expression: see section 13 14 15 16 moderate expression: see section 12

spinal cord mantle layer

strong

spotted

strong expression: see section 09 10 11 12 13 14 15 16

anterior naris

strong

regional

strong expression: see section 13 14 moderate expression: see section 12 15

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 17 18 moderate expression: see section 09 10 11 12 13 16

heart atrium

strong

spotted

strong expression: see section 20 21 22 moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

heart ventricle

moderate

spotted

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

tongue muscle

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail paraxial mesenchyme

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6430
Entity Detected:	Lpl, lipoprotein lipase ( MGI:96820)
Sequence:	sense strand is shown >T6430 GATTTCTCCTCCTACTCCTCCTCCGAGGAATTCTGCCCCCTGTAACTGTTCTGCCCTCCCCTTTAAAGGT TGACTTGCCCTACGGCGCTCCACCGCGCTCCAGTCCTCTTGCGCCTCCTGCTCAACCCGCTCCTGACTGC CCCACGCCGCGTAGTTCCAGCAGCAAAGCAGAAGGGTGCACCGGGAGATGGAGAGCAAAGCCCTGCTCCT GGTGGTCCTGGGAGTTTGGCTCCAGAGTTTGACCGCCTTCCGAGGAGGGGTGGCCGCAGCAGACGCAGGA AGAGATTTCTCAGACATCGAAAGCAAATTTGCCCTAAGGACCCCTGAAGACACAGCTGAGGACACTTGTC ATCTCATTCCTGGATTAGCAGACTCTGTGTCTAACTGCCACTTCAACCACAGCAGCAAGACCTTCGTGGT GATCCATGGATGGACGGTAACGGGAATGTATGAGAGTTGGGTGCCCAAACTTGTGGCCGCCCTGTACAAG AGAGAACCTGACTCCAATGTCATTGTAGTAGACTGGTTGTATCGGGCCCAGCAACATTATCCAGTGTCAG CTGGCTACACCAAGCTGGTGGGAAATGATGTGGCCAGATTCATCAACTGGATGGAGGAGGAGTTTAACTA CCCCCTAGACAACGTCCACCTCTTAGGGTACAGCCTTGGAGCCCATGCTGCTGGCGTAGCAGGAAGTCTG ACCAATAAGAAGGTCAATAGAATTACTGGTTTGGATCCAGCTGGGCCTAACTTTGAGTATGCAGAAGCCC CCAGTCGCCTTTCTCCTGATGACGCTGATTTTGTAGATGTCTTACACACATTTACCAGGGGGTCACCTGG TCGAAGTATTGGGATCCAGAAACCAGTGGGGCATGTTGACATTTATCCCAATGGAGGCACTTTCCAGCCA GGATGCAACATTGGAGAAGCCATCCGTGTGATTGCAGAGAGAGGACTCGGAGACGTGGACCAGCTGGTGA AGTGCTCGCATGAGCGCTCCATTCATCTCTTCATTGACTCCCTGCTGAATGAAGAAAACCCCAGCAAAGC ATACAGGTGCAACTCCAAGGAAGCCTTTGAGAAAGGGCTCTGCCTGAGTTGTAGAAAGAATCGCTGTAAC AATCTGGGCTATGAGATCAACAAGGTCAGAGCCAAGAGAAGCAGCAAGATGTACCTGAAGACTCGCTCTC AGATGCCCTACAAAGTGTTCCATTACCAAGTCAAGATTCACTTTTCTGGGACTGAGAATGGCAAGCAACA CAACCAGGCCTTCGAAATTTCTCTGTACGGCACAGTGGCCGAGAGCGAGAACATTCCCTTCACCCTGCCC GAGGTTTCCACAAATAAAACCTACTCCTTCTTGATTTACACGGAGGTGGACATCGGAGAACTGCTCATGA TGAAGCTTAAGTGGATGAGCGACTCCTACTTCAGCTGGCCCGACTGGTGGAGCAGCCCCAGCTTCGTCAT CGAGAGGATCCGAGTGAAAGCCGGAGAGACTCAGAAAAAGGTCATCTTCTGTGCTAGGGAGAAAGTTTCT CATCTGCAGAAGGGAAAGGACTCAGCAGTGTTTGTGAAATGCCATGACAAGTCTCTGAAGAAGTCTGGCT GACACTGGACAAACAAACAAGAGAAGAAAGCATCCGAGTTCTTTGAAGACAGAAGAAAACAAAGTAAATT TAATTTAAAAAAATAATACCCTTGTTTGGGTGTTTGAAAGTGGGTTTTCCTGAGTATTAATCCCAGCTCT ATCTTGTTAGTTAAACAGAAGACAGTCTCAAATATTAAACGGTGGCTAACCCAGGGTGAGGAATCTAATG GCCCATAGCAGGTCTTCCAGCATCAGAAGACATCAGGCAGGAGAAACATGCTGTCTTGTATCCCTTAAGA AGGAATCATTTGTTCCCAACAATATAAGACTCCATCATGTGACCCATTTGGTCATGGTCTAAAATTAGTA AGAACTCTGAGGTTTTATATTGAGACCTTTTCAAAGTTTTCTCAAAGTCTAATATAGACAATATTTTTTG TGGCATGAGTCAGGTCCATTTCTTTAGCGGTTGAAACACCTGGCCTTTGCAACTAGTTTTTTTTACCATT GGGATATATTCCCCCCACCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3584788 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3584788 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7328 same embryo
	EMAGE:7326 same embryo
	EMAGE:7330 same embryo
	EMAGE:7327 same embryo
	EMAGE:7329 same embryo
	EurExpress:euxassay_004410 same experiment
	MGI:4825972 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source