EMAGE mouse gene expression spatial database

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EMAGE:7418

Rbm47 RNA binding motif protein 47 ( MGI:2384294)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004545. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004545_01	euxassay_004545_02	euxassay_004545_03	euxassay_004545_04

euxassay_004545_05	euxassay_004545_06	euxassay_004545_07	euxassay_004545_08	euxassay_004545_09

euxassay_004545_10	euxassay_004545_11	euxassay_004545_12	euxassay_004545_13	euxassay_004545_14

euxassay_004545_15	euxassay_004545_16	euxassay_004545_17	euxassay_004545_18	euxassay_004545_19

euxassay_004545_20	euxassay_004545_21	euxassay_004545_22	euxassay_004545_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7418_wholemount_strong.wlz

7418_wholemount_moderate.wlz

7418_wholemount_weak.wlz

7418_wholemount_possible.wlz

7418_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7418_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 06 07 08 09 16 17 18 19

pancreas

moderate

regional

moderate expression: see section 10 14 weak expression: see section 08 09

lateral ventricle choroid plexus

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

4th ventricle lateral recess

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 moderate expression: see section 04

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 15 16 17 18

stomach

moderate

regional

moderate expression: see section 05 06 07 09 10 11 weak expression: see section 03 04 08

hindgut

moderate

regional

moderate expression: see section 11 12 13 14

rectum

moderate

regional

moderate expression: see section 11

midgut

moderate

regional

moderate expression: see section 11 12 13 15 16 17

midgut loop

moderate

regional

moderate expression: see section 05 06 07 09 10 11 12 13 weak expression: see section 08

meckel's cartilage

weak

regional

weak expression: see section 07 08 09 10 11 15 16 17 18 19

kidney calyx

moderate

regional

moderate expression: see section 07 16 17 weak expression: see section 08 09 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7546
Entity Detected:	Rbm47, RNA binding motif protein 47 ( MGI:2384294)
Sequence:	sense strand is shown >T7546 CCCACGCGTCCGGGCAGGCCGTGGTCGTTCTTGCTCAGGTAACACACAAGTTAGAAGAACAGCTTGTTTC CTGCTACGCAGCCACCCTCAGCAGTTCCCAGCTCACTGAAGTTCGGGTTTGCCTCAGAGGACACGTCAAG AACTGATCAGAGAAATCCCAAGCGAGCTGCCTGAACCTTCGAGAATTCTCTTGTTCTACCTTCCATATAA AGAACCAGGACCAATCGCATTGGTGACCTTTGGTTTCCACCAGCTGTGGATGCCTTTGACAGTATGACTG CTGAAGATTCCGCCACAGCCATGAACAGCGATCCAACAGTGGGGTCCTCCACCAAGGTACCGGAGGGTGT GGCGGGCGCGCCCAATGAGGCTGCACTGTTGGCACTGATAGAGCGCACCGGCTACACCATGGTACAGGAG AACGGGCAGCGCAAGTACGGTGGACCCCCGCCCGGCTGGGAGGGCCCGCATCCCCAGCGTGGCTGTGAGG TCTTCGTGGGCAAGATCCCACGTGACGTGTATGAAGATGAGCTGGTGCCTGTGTTTGAGACCGTAGGTCG CATCTATGAGCTGCGACTCATGATGGATTTCGATGGCAAGAACCGCGGCTATGCCTTCGTGATGTACTGC CACAAGCATGAGGCCAAGCGAGCCGTTCGGGAACTCAACAACTACGAGATCCGCCCCGGCCGTCTGCTGG GTGTGTGCTGTAGTGTGGACAACTGCCGTCTCTTCATTGGTGGCATCCCCAAGATGAAGAAGCGCGGGGA GATCCTGGAGGAGATCGCCAAGGTCACCGAGGGCGTACTCAACGTGATTGTGTATGCCAGTGCGGCAGAC AAGATGAAAAACCGAGGCTTCGCCTTTGTGGAGTATGAGAGCCATCGTGCTGCAGCCATGGCACGCCGCA AGCTCATGCCGGGTCGGATCCAACTGTGGGGCCACCAGATTGCTGTGGACTGGGCCGAGCCAGAGATTGA CGTGGACGAAGATGTGATGCAGACAGTGAAGATCCTCTACGTGCGCAACCTCATGATCGAGACCACCGAG GAGACCATCAAGAAGAGCTTTGGCCAGTTCAACCCAGGCTGCGTGGAAAGGGTCAAGAAGATCCGTGACT ACGCCTTCGTGCACTTCACTAGCCGTGAGGATGCTGTGCATGCTATGAACAACCTCAACGGCACAGAGCT GGAGGGATCCTGCTTAGAAGTCACGCTGGCCAAGCCCGTGGACAAGGAACAGTACTCCCGCTACCAGAAG GCAGCCAAGGGTGGTGGTGGCTCGGCTGAGGCCGTAGCGCAGCAACCCAGCTACGTGTACTCCTGTGACC CCTACACGTTGGCCTACTACGGCTACCCCTACAACGCTCTCATCGGGCCCAACCGGGACTACTTCGTGAA AACAGGCAGCATAAGAGGCCGAGGGAGAGGCGCAGCTGGAAACAGGACCCCAGGGCCCAGGGGTTCCTAC CTCGGAGGATATTCCGCTGGCCGTGGTATATATAGCCGATATCACGAAGGCAAAGGCAAACAGCAGGAGA AAGGATATGAACTTGTGCCGAATTTGGAGATCTCTCCTGTCAATCCAGTTGCCATTAAACCTGGCACAGT GGCCATCCCTGCCATCGGTGCTCAGTACTCCATGTTCCAGGCAGCCCCAGCCCCGAAAATAATCGAAGAT GGCAAGATCCACACAATGGAACACATGATCAGCCCCATTGCGGTGCAGCCTGACCCCGCCACTGCTGCTG CCGCTGCAGCCGCTGCAGCCGCTGCTGCCGTCATTCCTGCGGTATCCACACCGCCACCTTTCCAGGGCCG TCCAATAACTCCTGTGTACACCGTGGCACCAAATGTTCAGAGAATCCCCACCGCCGGCATCTACGGGGCC AGCTACGTCCCCTTTGCTGCTCCCGCCACTGCCACAATCGCCACACTACAGAAGAACGCCGCGGCTGCCG TGTATGGGGGCTACGCCGGCTACATACCTCAGGCCTTCCCCGCTGCTCTCCAGGTGCCCATCCACGACGT CTACCAGACTTACTGAGAACGGGGGACCGGAGCCAAGGCAAGCCACCAAACCCCGCTGAAGGAACACTTT ATTATTTATGAAGAAAGAACGTGTCGGGTTGGTACACGTGCAAAACCTGCAGGTGAAGAAGAATGTTATC AAATAGCACACTGAAATATTTTATTATATCCATGAAGTTTTCACTCGCTTTTTTTTTTTTTTTTTTTTTT GAAAATGACTTTTCAATTTAGCAGGCCCGCGTTCATACATTTCTAAAAGACTTGCGACGCCTCACGCCTT AATGCCATCTCTTAAAGTTTGTATGCTCTTCTGCTTTTGTACAGAGGTTTGGTTTTTTGTTTTTTAAGGA TATATTTTCAGTATGAAGGTTATTTTCTTAACTTCTGCACTCCAGAGTTTTCTATTTTGTAGAACCTTTC AAAGTATATCAGCTATATGTATGTATATCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4240441 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4240441 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7422 same embryo
	EMAGE:7420 same embryo
	EMAGE:7423 same embryo
	EMAGE:7419 same embryo
	EMAGE:7421 same embryo
	EurExpress:euxassay_004545 same experiment
	MGI:4827654 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source