EMAGE mouse gene expression spatial database

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EMAGE:7543

Slc35e3 solute carrier family 35, member E3 ( MGI:2448489)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004678. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004678_01	euxassay_004678_02	euxassay_004678_03	euxassay_004678_04

euxassay_004678_05	euxassay_004678_06	euxassay_004678_07	euxassay_004678_08	euxassay_004678_09

euxassay_004678_10	euxassay_004678_11	euxassay_004678_12	euxassay_004678_13	euxassay_004678_14

euxassay_004678_15	euxassay_004678_16	euxassay_004678_17	euxassay_004678_18	euxassay_004678_19

euxassay_004678_20	euxassay_004678_21	euxassay_004678_22	euxassay_004678_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7543_wholemount_strong.wlz

7543_wholemount_moderate.wlz

7543_wholemount_weak.wlz

7543_wholemount_possible.wlz

7543_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7543_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

cervical intervertebral disc

strong

regional

strong expression: see section 16 moderate expression: see section 08

lumbar intervertebral disc

strong

regional

strong expression: see section 11 12 moderate expression: see section 10

thoracic intervertebral disc

strong

regional

strong expression: see section 11 17 moderate expression: see section 07 09 10 14

forearm mesenchyme

strong

regional

strong expression: see section 01

upper arm mesenchyme

strong

regional

strong expression: see section 03 04 05 20 21

hand mesenchyme

strong

regional

strong expression: see section 02 03 21 22 23

hindlimb digit 1 metatarsal

strong

regional

strong expression: see section 04 05 22

hindlimb digit 1 phalanx

strong

regional

strong expression: see section 06 22

hindlimb digit 2 metatarsal

strong

regional

strong expression: see section 04 05 23

hindlimb digit 2 phalanx

strong

regional

strong expression: see section 06 07 23

hindlimb digit 3 metatarsal

strong

regional

strong expression: see section 04 05 23

hindlimb digit 3 phalanx

strong

regional

strong expression: see section 06 07 23

hindlimb digit 4 metatarsal

strong

regional

strong expression: see section 04 05 23

hindlimb digit 4 phalanx

strong

regional

strong expression: see section 06 23

hindlimb digit 5 metatarsal

strong

regional

strong expression: see section 05 22

hindlimb digit 5 phalanx

strong

regional

strong expression: see section 06 22

foot mesenchyme

strong

regional

strong expression: see section 04 05 22 23

lower leg mesenchyme

strong

regional

strong expression: see section 01 02 03 23

upper leg mesenchyme

strong

regional

strong expression: see section 01 02 03 06 07 18 22 23

otic capsule

strong

regional

strong expression: see section 19 20 21 22 moderate expression: see section 01 02 03 04 05 06 07 08 15 16 17 weak expression: see section 18

nasal septum

strong

regional

strong expression: see section 08 09 10 11 12 13

viscerocranium

strong

regional

Expression in the turbinate bone.

stomach

weak

regional

weak expression: see section 04 05 06 07 08

cricoid cartilage

strong

regional

strong expression: see section 12 13

thyroid cartilage

strong

regional

strong expression: see section 10 11 12 14

trachea

moderate

regional

moderate expression: see section 13

sacral vertebral cartilage condensation

strong

regional

strong expression: see section 11 12 moderate expression: see section 10

cranium

strong

regional

strong expression: see section 01 02 03 04 05 06 11 12 16 17 18 19 20 21 22 23

clavicle

strong

regional

strong expression: see section 09 10 11 15 moderate expression: see section 16

axial skeleton tail region

strong

regional

strong expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8635
Entity Detected:	Slc35e3, solute carrier family 35, member E3 ( MGI:2448489)
Sequence:	sense strand is shown >T8635 AGCGACCGGGACCCGGCTGGCGCATGCCTGTCTCCGGCCGGACCGTGGAGCGGGCGGCTGCGGGCGCGCG GCGGCGGAGAGCCGGGACAGCGGGAGAGCGTCCGAGCGCCCCGCGGGGAGGCTGGACAGCCCGGGCTCGG CCGAGGACGCTGCTCCGCAGCCGGCGGGCATCATGGCATCGCTGGCGGACCGGGTCCGGGGCAACGGGCG CATCGCCGCCGGGCTCCTGTTCAACCTGCTGGTGTCCATCTGCATCGTGTTCCTCAACAAATGGATCTAT GTACACCACGGCTTCCCCAACATGAGCCTGACCCTGGTGCACTTCGTGGTCACCTGGCTGGGCTTGTACA TCTGCCAGAAACTGAACATCTTTGCCCCCAAAAGTCTGCCGCTCTCCAAGCTCCTCCTCCTGGCCCTCAG CTTCTGTGGCTTTGTGGTCTTCACCAACCTTTCTCTGCAGAACAACACCATAGGCACCTATCAGCTGGCC AAGGCCATGACCACGCCGGTGATCATAGCCATCCAAACCTTCTGGTACCAGAAGAGATTCTCCGTCAGAA TACAGCTCACGCTGATTCCTATTACTGTAGGTGTAATCCTAAATTCTTATTACGATGTGAAGTTTCATTC GCTTGGAATGGTGTTTGCCGCCCTTGGTGTGGTAGTCACGTCCCTTTATCAAGTGTGGGTCGGAGCCAAG CAGCACGAACTGCAGGTGAACTCCATGCAGCTACTTTACTACCAGGCCCCCATGTCTTCTGCCATGCTGC TGGTGGCTGTGCCCTTCTTCGAGCCAGTGTTTGCAGAGGGAGGAATATTTGGTCCCTGGTCAGTTTCTGC TTTGCTTATGGTACTGCTGTCTGGAATAATCGCTTTCATGGTGAACTTATCGATTTACTGGATCATTGGG AACACGTCACCAGTCACCTACAACATGTTCGGACACTTCAAGTTCTGCATCACTCTCTGCGGAGGATACA TTCTATTTAAGGACCCCCTGTCGGTAAACCAGGGACTCGGCATCCTATGCACGCTCTTCGGTATCCTCAC CTACACCCACTTTAAACTCAGCGAGCAGGAAGGCAGTAAGAGTAAGCTGGTCCAACGGCCCTGAGCTTTG GCAGAGAAGAGAAAGGCGAATCATGAGGATGAGAAGCGACAAACGTGTGCGTCGTCTATGGAAAAGCTCC CCGTGGAATTTAGTCAGTGACGTACAAGAAGTATTTTCTTTGCAGATAATGACAATTTTTAGTACCTTTT ATCGATTTAATTTTTAAAAACCAAAATGGTTCATTCAGGCTTCTCAAAGACTTAATGGACCTGGAGCTGG CACTCGGTAGTAGAGTGTTTGCCTCACACGCGTGAGACCCTGGCCTCAAACTCCAGCACCGTCAAAGAAA TGCTAGCAACTGCCTAGAAGATGGCTTCATCCTGCCAGCTGAAGCACGGGAGAGTTCGGCTCCATAGGAA TGACAGAGCGCTCCTTCGCTGCAGCTGTGCCGTGGCTGTCGGTGTCCAGTGTCCCTGTTCTCCCAGGAAG GGCAAGCCAACCTGCTGGCTCACCCAGGCCTTCCTGTCGGGAAGTTGAAGTTCTTTCTCGTTCGGACAGG GTGGTGGTCAGAGTTGTATTGAGACTGGATCTCACTGTGTCGCTCTGACTGGTCTGGAATTCTCTACATA GCCCAAACTGGCTTTGGACTCTACCACTCCATGTAGCCTTTAGCTTTCTGATTTCACTGCAGAACTGAAA CGTTTATAGAAAGGAAGCTGTCATCGACATGCTCATGAAACCAATTACATCTCTGAAAGTATCACCTTTT CAGTAGAAACTTAATCTAAGACATTCTTCCCTGTTAGACAGGGCAGGAGTGCTTGAGCCCAAAGCCCGCT CAGCAGCACAGCTCTGCCCGTGCTAGAGGGAGCGTCTGCAGACTGACAGTCTCATTGGTCTTCTCGCGGC TTTGGGCTTTGAGTGAGTTCTAGATACTATATATGGAGTTACAGGAAAACCCATCTTTAAACGGCTCAAA GCAAAGCTGTAGAGGGCCAGCAGGTAGAGCCAGTAACCGGGTCCCTGCCCAGCACTCATAGGTGGGTTCG CAGTCGTCCTGGTAACTGCTTCCCCAGCTTGCTTAATCCTTTCATCCCAGTGTTTTCTGATGTGGCCTCC CTCCTGTACTCCCTGCCTCCCACATCCTGAGCAGTAGATAAGGAGCGTTGTATGTATTGGAAAAACTGGA CTGAACCAGGCCTTGCAGTTTATGTGGCAAATCAGAGCAAAGGCTGCCCAAGTTCTAGGCAGAAACTTGG TGAAAAACAGGAAAGATATGAAAGACAGGTAGAAAACTAGCTAATATTTAGCTACTTGTATGAGGTTCCA GAGGAATTGAGAAATGTTTTTAGTTGAGATGTAGACAGAATACATGGGGCTCCAGCAAATAGAGCCCTGG GTATTTAACTGCAAAAGTGTGTGTCTGCCTTCAGGTATCTTGTCGTTTTGCCATTCACAGCAGCCGCTGA TGACCGCAGAAGAGTCGGGTCTCTTGGAATAATAACTTTTTCTAAGCATACACTTCCTGTGAGAAAGCAG CTTAAGACAAAGAGACGTGTCTTGTGATAATACATCTTATCTGGAATTTTCTTCTTCACAGGAAGAGGTA ACTTTTAATATATTAGGATTTTTTGTTGGTAGTGGTAGTTTTTAGGTTTTTTTTTTTTTTAACCAAATAA AAAAGCCTGGGTTGTATCCCCAGCACTGCCAAAACAAAGCCTTTTCAAGTCATCAGGCCACAGGAGTGCT GAGCTCGAGGAGAAGACACACCCCTAGGTCTCAAGGTTGGGCAGTGAGTCAGGGCTACGAGTTCCTTGGC CGTGTAGACCGCACAGACTTCCAGGGCAGCCCAAATCACATACTGAGTTCTGTTAAGGGGAAAGGAAGGG TGCCTGGTCTAGGACTTTTTTTTTTTTTCCTGGCCTCACTAAATGTGTATTTTGGAGAATGGCATTGTCT GCTGTGTATCAGTCCATGTGCTTCCGTGGGTCTTTGTTTTAAATATTGTCCTCAGTTTACAGAGTTGTGT TATTATGCCAAGAACTGCAGAACATGCCGTCTTCACCATCATGGACCTTTGGAATCATGCTCAAGAAACA TTTTCTGTGTATGTTTAATGTTTAACAGTTTCCCAGAAGCTAACGGAAAGTCTTCCAGCACAGAGAGCCT CAGGTTTCTGTGCATTAGGAACAACGGAGTTGATTTGTACTGCGTACAAATCCTAGCTGTAGATAAGCCC TGGGGACCGGAGTTGATTTGTACTGCGTACAAATCCTAGCTGTAGATAAGCCCTGGGGACCGGGGTGGTT TGAATAGGTAGGTGGGTGGGGTATGATAAGTGGCTGGTGTCAGTAGGAATGAGGGACAGAGTGGGAGTAT GGTGAATCAAGGCGTGTTTATATACGTGAATAAACTCTCTACTATTAGAACCAAAAAAAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5720169 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5720169 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7542 same embryo
	EMAGE:7541 same embryo
	EMAGE:7544 same embryo
	EMAGE:7545 same embryo
	EMAGE:7546 same embryo
	EurExpress:euxassay_004678 same experiment
	MGI:4828202 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source