EMAGE mouse gene expression spatial database

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EMAGE:7607

Wnt7b wingless-related MMTV integration site 7B ( MGI:98962)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004708. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004708_01	euxassay_004708_02	euxassay_004708_03	euxassay_004708_04

euxassay_004708_05	euxassay_004708_06	euxassay_004708_07	euxassay_004708_08	euxassay_004708_09

euxassay_004708_10	euxassay_004708_11	euxassay_004708_12	euxassay_004708_13	euxassay_004708_14

euxassay_004708_15	euxassay_004708_16	euxassay_004708_17	euxassay_004708_18	euxassay_004708_19

euxassay_004708_20	euxassay_004708_21	euxassay_004708_22	euxassay_004708_23	euxassay_004708_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7607_wholemount_strong.wlz

7607_wholemount_moderate.wlz

7607_wholemount_weak.wlz

7607_wholemount_possible.wlz

7607_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7607_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

not examined

regional

not examined expression: see section 01

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

4th ventricle

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17

spinal cord ventricular layer

strong

regional

strong expression: see section 10 11 12 13

inner ear

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 24 moderate expression: see section 17 18 19 20 21 22 23

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 21 22 23 24

eye

moderate

regional

moderate expression: see section 03

cornea

moderate

regional

moderate expression: see section 03 04

lens

strong

regional

strong expression: see section 01 02 24

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 11 12 15 16 17 18

nasal cavity respiratory epithelium

weak

regional

weak expression: see section 11 12 15

oral epithelium

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 16 17 18 19 20

lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

trachea

strong

regional

strong expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8680
Entity Detected:	Wnt7b, wingless-related MMTV integration site 7B ( MGI:98962)
Sequence:	sense strand is shown >T8680 CGCCCGCCTCCCGAGCCGAAGCGCCGGCTGAGCGTGGTCCTACCGCAGCTCCCTGGCTCCTGCCCGGCCC CTGCCCACCCGCGCGTCCCCTCCGGCCGCAGCTGTCTATGGCGCAGCCCCCCTCCCTGGATCATGCACAG AAACTTTCGAAAGTGGATCTTTTACGTGTTTCTCTGCTTTGGCGTCCTCTACGTGAAGCTCGGAGCATTG TCATCCGTGGTGGCCCTGGTAGCCAACATCATCTGCAACAAGATTCCTGGCCTGGCCCCACGGCAGCGTG CCATCTGCCAGAGCCGACCCGATGCCATCATTGTGATCGGGGAGGGGGCGCAGATGGGCATCGACGAGTG CCAGCACCAGTTCCGATTCGGCCGCTGGAACTGCTCCGCCCTGGGCGAGAAGACCGTCTTCGGGCAAGAA CTCCGAGTAGGGAGTCGAGAGGCTGCCTTCACCTATGCCATCACGGCGGCGGGCGTGGCGCATGCTGTCA CCGCTGCCTGCAGCCAGGGCAATCTGAGCAATTGTGGCTGTGACCGGGAGAAGCAAGGCTACTACAACCA GGCGGAAGGCTGGAAGTGGGGGGGCTGCTCAGCGGACGTCCGCTACGGCATCGACTTTTCTCGTCGCTTT GTGGATGCCCGTGAGATCAAAAAGAACGCCAGGCGCCTCATGAACCTTCACAACAATGAGGCGGGCAGAA AGGTTCTGGAGGACCGCATGAAGCTGGAATGTAAGTGTCACGGTGTGTCAGGCTCCTGTACCACCAAAAC TTGCTGGACCACGCTACCTAAGTTCCGCGAGGTGGGCCACCTGCTCAAGGAGAAGTACAACGCAGCGGTG CAGGTGGAGGTGGTGCGAGCCAGCCGCCTGCGCCAGCCCACCTTCCTGCGCATCAAGCAGCTACGCAGCT ACCAGAAGCCTATGGAGACGGACCTGGTGTACATCGAGAAGTCGCCCAACTACTGCGAGGAGGACGCGGC CACGGGCAGCGTGGGCACGCAGGGCCGTCTGTGCAACCGCACCTCGCCGGGGGCCGACGGCTGTGACACC ATGTGCTGCGGCCGCGGCTACAACACGCACCAGTACACCAAGGTGTGGCAGTGTAACTGCAAATTCCACT GGTGTTGCTTCGTCAAGTGCAACACGTGCAGCGAGCGCACCGAGGTCTTCACCTGCAAGTGAGGCTCCCG CGCAGGCGCGCTCGGCCCCTGCCGACCCTGCGGCCCTCGCCATTATTTTGCACATCCTTCTTTGCTTCTG GAGCTGCCAGCTGCAGGCACAGGAGGGTGGGGATAGAGGTGGGGAGCTCGAGATACTCCAGGCTCCTTCC TACTCGCTCTGTCCCCGCCCAGCATCCAAGGTCAACGCAATGGTGGTCTGGTACCCAATGGAGACAAATC CCTTTACTTCTCTTTGGGAAAGTGAACCACAAAGGGACCATGAGACTCTGAGGGTCACCTCCCTGCCTGT GACTGGACACAGAAAGGCCACACCCACCAGTCACACTCAAAACGGTTTCCTGGGCTGTTTCCTGCCGGCC CTGGGCAGTGTGGATGGATGTTGACAAAATTATTTATGTTTTCTTAGCATCAGATGAGGACTCAGTACTA ACGACTGGGTAGCCAGACCTAACCCTATTTGAGGACACCCTTCCCTCACTCCTCCCGGCCCCTCCCTGCA GGGTCCTCTGCTCCTTGCAGAACTCGAGGATGTCAGAATTGGCACGGAAGCTGGCTGGTGGGGGGACTCC TTATCAGCACCTTGGGAGGGGCTTGGTGGCCCTACAAGGCCTGAGATGGCCGCAGAGGACAGCCAATCTT CCATTCCATTTGGAGACTGTCATGCAAATCAAATGTCCCTTGTGTCAGGCTCCAGGCATGCCTCGTCCTC TCCCTGGTCCTTCACCCTCCCAGCCTGCTGCCAACCTCCACCTCCAGTTTACAAATTCTCTTCTCCTCTG GAGCCAACCTGACACCCAGGACTGCCCCACAGGTTCAGGAGAGGTCAGGGACAGTTGCCCCACATGACAG ATGGACAGAGGGCAATCTGAAGATTTACTGGAGACCCCACGGCTCTGTGAAATAAATATACTGACACAGC CCCATCCAGCCCAACTCTGGAAGTTGCCAGGGTGATGGGAGGCTGCACCCCCTTTTCAGTACCTTGGGTT TTGTCCTTCTTCTGTGATCCTGATGCCAGAGAACTGACATCCAGAATTTAGGGATGTATTGGTCAGGCCC CCTGCCTAGTGTCCACTGATACCTGCTTCAGGGTCCTTATATTATGAGGACATGGGACCCTCAAACAGGG GTCCGTGGGAAGCTTAATGTCCCATTTCCTCAGGCCCTTCCAGATGGGGACAGAAGAACTCAGGCCTGGG CATATCCCACCCTTTCCTCCACAACACATGGCAGGGTAAGAAACTGCCAGGGCTGATAATACAACTGCCC ACAGCCTACCCCACACTAAGGTGTTTCATAGCAGAAGTCCATGGAAATGTGGGGTTTGGTGGCCACCAAG CCAGGTGGCCTGGACATTGACCTGGGGAAGGTGACCCTTGTTTGCCCTTGCCTTGCATCCAGCTGTGTGT CCCTATCATGTCAGGATGTTCCAAGCCTCTGGGCCACTGGAAATGTCCCACCCTGATCCTGGCCCCATCT CCTCACCCCAAGTCCTGGGATACCCACGTCCGTCGCCCAGTGTCCCCTGTGAGGAGCCTGGTTAACTTAT ATTGTTATATAGCGTCCCCTGTCTGTCATGTCTCTTAAGTTATTGTGACCTACACTGGGTACCGGAGGGG ATGGGGGATGGCTTCAGCTGCTGTCCCCCAAGCCAGGCTCCTCCTTCTGCTTGAAACAGACCCTCGGGGG CCCCTGATGCCACCGAGGCAATTCGCACTGTCCCTGGGCTGCCAGGCACCTGCGCCTGCACTCGGTCAGC CGCAGACCTTGCCTTGGGGGAGAGAGGTGGTTAGTGGACCCAGGCAGGGCACTGGCTGTCCCAATGCTGT GTGCTGGGGTGGAGGTGGCCGGGCACCACATGTCCTTGAAGTGCCCTACTTCTGATGGGCTGTGTTCCTG CCTCCTCTGGAGGGGAGCACTTAGCCCCAATAAAAGCTGGAATCAGAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:6401357 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6401357 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7611 same embryo
	EMAGE:7608 same embryo
	EMAGE:7610 same embryo
	EMAGE:7612 same embryo
	EMAGE:7609 same embryo
	EurExpress:euxassay_004708 same experiment
	MGI:4829227 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source