EMAGE mouse gene expression spatial database

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EMAGE:7623

Hhip Hedgehog-interacting protein ( MGI:1341847)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004722. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004722_01	euxassay_004722_02	euxassay_004722_03	euxassay_004722_04

euxassay_004722_05	euxassay_004722_06	euxassay_004722_07	euxassay_004722_08	euxassay_004722_09

euxassay_004722_10	euxassay_004722_11	euxassay_004722_12	euxassay_004722_13	euxassay_004722_14

euxassay_004722_15	euxassay_004722_16	euxassay_004722_17	euxassay_004722_18	euxassay_004722_19

euxassay_004722_20	euxassay_004722_21	euxassay_004722_22	euxassay_004722_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7623_wholemount_strong.wlz

7623_wholemount_moderate.wlz

7623_wholemount_weak.wlz

7623_wholemount_possible.wlz

7623_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7623_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 14 15 16 17 18 19 20 21 22 23

hand

strong

regional

strong expression: see section 01 20 21 22 23

foot

strong

regional

strong expression: see section 01 03 04 05 19 20 21 22

fibula

strong

regional

strong expression: see section 02 23

tibia

strong

regional

strong expression: see section 02 23

femur

strong

regional

strong expression: see section 01 02 03 04 20 21

vibrissa

strong

regional

strong expression: see section 05 06 07 18 19 20

otic capsule

strong

regional

strong expression: see section 03 moderate expression: see section 04 07 08 15 16

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 16 17 moderate expression: see section 07 08

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 11

pharynx

strong

regional

strong expression: see section 10 13 moderate expression: see section 11 12

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06

midgut

moderate

regional

moderate expression: see section 06 07 08 09 10 11 13 14 15 16 17

mandible

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

maxilla

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 23

palatal shelf

strong

regional

strong expression: see section 13 14 15 16 17 moderate expression: see section 08 09 10 11 12

bladder

moderate

regional

moderate expression: see section 11 12 13 14

lung

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 21 22 moderate expression: see section 11 12

trachea

moderate

regional

moderate expression: see section 11 12

axial skeleton

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

cranium

strong

regional

strong expression: see section 01 02 03 04 05 18 19 20 21 22 23

clavicle

strong

regional

strong expression: see section 15 16 moderate expression: see section 07 08 14

scapula

strong

regional

strong expression: see section 01 02 moderate expression: see section 03

pelvic girdle skeleton

strong

regional

strong expression: see section 15 16 moderate expression: see section 07 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8704
Entity Detected:	Hhip, Hedgehog-interacting protein ( MGI:1341847)
Sequence:	sense strand is shown >T8704 AGAAGCTGCAGCCGCCGGCAGAGGAGACCTCAGCATCCTCGGGAGCCCAGCGCCAACCCTGCCTCCGCCC GGCCCGCTGCCACCACCGCCGCCCTTTCGGTTCCTGCTACTGTCTCACCTAAACAACTCCCATTCAGCGG ACAAGCGAAGTTCTATGACTCTCTTCTCCTCTCCTTCCTCCTCTTCTTCCAACTCCTTCTCCTCCCACTT CCCAACCGCTGTGGAAAGCCCCTAACCCAACAGACGCTGGCAAGGCTGCGGACAAGTGTCATCTGCACTT TATCTTGCTGCTCCTACTGCCCTAAGGCAAAGTTGCATAGCTCTACATCTTTCTTTCCCAGCCACCTCCC TCTGCCCCCAAGAGCGTCCCGCCGCCCCGCAGCACTCTCCTGGAGCTGCGCCCTAGTGCCCCTGCTGGGC AGTGGCCTTTCCCCCACCCCATCCTCCCGCGTCCTGCCCTTGCTGCTCCGGGCAGACGATGCTGAAGATG CTCTCGTTTAAGCTGCTACTGCTGGCCGTGGCTCTGGGCTTCTTTGAAGGAGATGCGAAGTTTGGGGAAA GGAACGAGGGGAGCGGAGCGAGAAGGAGACGGTGCCTGAATGGGAACCCCCCAAAGCGCCTAAAGAGAAG GGACAGGCGGGTGATGTCCCAGCTGGAGCTGCTCAGTGGAGGAGAGATCCTGTGTGGTGGCTTCTACCCA CGAGTATCTTGCTGCCTGCAGAGTGACAGCCCTGGATTGGGGCGTCTGGAGAACAAGATCTTTTCTGCCA CCAACAACTCAGAATGCAGCAGGCTGCTGGAGGAGATCCAATGTGCTCCCTGCTCCCCGCATTCCCAGAG CCTCTTCTACACACCTGAAAGAGATGTCCTGGATGGGGACCTAGCACTTCCACTCCTCTGCAAAGACTAC TGCAAAGAATTCTTTTATACTTGCCGAGGCCATATTCCAGGTCTTCTTCAAACAACTGCTGATGAATTTT GCTTTTACTATGCAAGAAAAGATGCTGGGTTATGCTTTCCAGACTTCCCGAGAAAGCAAGTCAGAGGACC AGCATCTAACTACTTGGGCCAGATGGAAGACTACGAGAAAGTGGGGGGGATCAGCAGAAAACACAAACAC AACTGCCTCTGTGTCCAGGAGGTCATGAGTGGGCTGCGGCAGCCTGTGAGCGCTGTGCACAGCGGGGATG GCTCCCATCGGCTCTTCATTCTAGAGAAGGAAGGCTACGTGAAGATTCTAACCCCAGAAGGAGAACTGTT CAAGGAGCCTTACTTGGACATTCACAAACTTGTTCAAAGTGGAATAAAGGGAGGAGACGAAAGGGGCCTG CTAAGCCTGGCATTCCATCCCAATTACAAGAAAAATGGAAAGCTGTATGTGTCTTATACCACCAACCAGG AACGGTGGGCTATTGGGCCTCACGACCACATTCTTCGGGTTGTGGAATACACAGTATCCAGGAAAAACCC CCATCAAGTTGATGTGAGAACAGCCAGGGTGTTTCTGGAAGTCGCAGAGCTCCACCGAAAGCATCTTGGG GGACAGCTGCTCTTTGGTCCTGATGGCTTTTTGTACATCATCCTTGGGGATGGTATGATCACATTGGATG ACATGGAAGAGATGGATGGGTTAAGTGACTTCACAGGCTCTGTGCTGAGGCTGGACGTGGACACCGACAT GTGCAATGTGCCTTATTCCATACCTCGGAGTAACCCTCACTTCAACAGCACCAACCAGCCCCCAGAAGTA TTTGCCCACGGCCTCCATGATCCAGGCAGATGTGCCGTGGATCGACATCCTACTGATATAAACATCAATT TAACAATACTTTGCTCAGATTCCAACGGGAAAAACAGGTCATCAGCCAGAATCCTACAGATAATAAAGGG AAGAGGTTATGAAAGTGAGCCATCTCTTCTTGAATTCAAGCCATTCAGTAACGGCCCTTTGGTTGGTGGA TTTGTTTACAGAGGCTGTCAGTCTGAAAGATTGTACGGAAGCTATGTGTTCGGAGATCGCAATGGGAATT TCTTAACCCTCCAGCAAAGCCCAGTGACCAAGCAATGGCAAGAAAAGCCGCTCTGCCTGGGTGCCAGCAG CTCCTGTCGAGGCTACTTTTCGGGTCACATCTTGGGATTTGGAGAAGATGAATTAGGAGAGGTTTACATT CTATCAAGCAGTAAGAGTATGACCCAGACTCACAATGGAAAACTCTACAAGATCGTAGACCCCAAAAGAC CTTTAATGCCTGAGGAATGCAGAGTCACAGTTCAACCTGCCCAGCCACTGACCTCCGATTGCTCCCGGCT CTGTCGAAACGGCTACTACACCCCCACTGGCAAGTGCTGCTGCAGTCCCGGCTGGGAGGGAGACTTCTGC AGAATTGCCAAGTGTGAGCCAGCGTGCCGTCATGGAGGTGTCTGTGTCAGACCGAACAAGTGCCTCTGTA AAAAGGGCTATCTTGGTCCTCAATGTGAACAAGTGGACAGGAACGTCCGCAGAGTGACCAGGGCAGGTAT CCTTGATCAGATCATTGACATGACGTCTTACTTGCTGGATCTCACAAGTTACATTGTATAGTTTCTGGGA CAGTTCGAGTCTATCTTTCCAGTGGGCATTTATTTTGACCTTGTCATCATTAAAAAGAGAGACTGTCCTT CTGCTACACACCTCTGTGATTTCATTCTTCTTTTAGTAATTTAAAAGCAATTATCAGAAATGTACAGCTC TCAGCATGTATGTTGGCACATTTCTTCACATCTGCATACACACGCACAACCCCAAACTCGAGGTTGTATA ACAGAAGGATTTTTTTGTTGTGTTTTGTTTTGTTTTTAATATCTACTTGCTGGTATGGAGTACTTTACAC AGAAGTTCCACTGCAAATTCGTCACAGAGTTTTTTATGGCATTGGAAGATATTCTTTTACATTTCAGGAA ATTCTGTCTGTAATTACTAAAGTTGACCTTAGTAGGGTTTTGAAATGTTCTTGTGCAATGTAGTGGAAGT ATTTTTATATTAAAGGTCTATAATAAGAGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6402422 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6402422 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7620 same embryo
	EMAGE:7624 same embryo
	EMAGE:7622 same embryo
	EMAGE:7621 same embryo
	EMAGE:7619 same embryo
	EurExpress:euxassay_004722 same experiment
	MGI:4825371 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source