EMAGE mouse gene expression spatial database

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EMAGE:7629

Grm4 glutamate receptor, metabotropic 4 ( MGI:1351341)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004721. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004721_01	euxassay_004721_02	euxassay_004721_03	euxassay_004721_04

euxassay_004721_05	euxassay_004721_06	euxassay_004721_07	euxassay_004721_08	euxassay_004721_09

euxassay_004721_10	euxassay_004721_11	euxassay_004721_12	euxassay_004721_13	euxassay_004721_14

euxassay_004721_15	euxassay_004721_16	euxassay_004721_17	euxassay_004721_18	euxassay_004721_19

euxassay_004721_20	euxassay_004721_21	euxassay_004721_22	euxassay_004721_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7629_wholemount_strong.wlz

7629_wholemount_moderate.wlz

7629_wholemount_weak.wlz

7629_wholemount_possible.wlz

7629_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7629_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 17 18 19 20 21 22 23

humerus

moderate

regional

moderate expression: see section 23

foot

moderate

regional

moderate expression: see section 19 20 21 22 weak expression: see section 01 02

fibula

moderate

regional

moderate expression: see section 21 22 23

tibia

moderate

regional

moderate expression: see section 21 22 23

femur

moderate

regional

moderate expression: see section 01 19 20 weak expression: see section 17 18

brain

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 02

trigeminal v ganglion

moderate

regional

moderate expression: see section 20 21 weak expression: see section 03 04 05 06 07 08 16 17 18 19

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 08 15 16

mandible

moderate

regional

moderate expression: see section 04 05 06 07 08 15 16 17 18 19 20 weak expression: see section 03 14

meckel's cartilage

weak

regional

weak expression: see section 03 04 05 06 07 08 09 11 12 13 14 16 17 18 19

upper jaw skeleton

moderate

regional

moderate expression: see section 08

maxilla

moderate

regional

moderate expression: see section 04 05 06 07 16 17 18 19 weak expression: see section 08

axial skeleton

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

scapula

moderate

regional

moderate expression: see section 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8707
Entity Detected:	Grm4, glutamate receptor, metabotropic 4 ( MGI:1351341)
Sequence:	sense strand is shown >T8707 AATGGTCCACCCCATCGGCCTTCAGCAGCCATCCTGTTGCTGAAGCTGCGCTGTCCATGTACACCCAGAT CATGGGACCCAGGGCCTGCTAGGGCTAGGAGTGGGGCCCGCTATTCATGGGTCTCTAAGCCTTTCCGAAA TGTCCGGGAAGGGAGGCTGGGCCTGGTGGTGGGCCCGGCTGCCCCTCTGCCTACTCCTCAGCCTTTATGG CTCCTGGGTGCCTTCATCCCTAGGAAAGCCCAAGGGTCACCCCCACATGAACTCTATCCGTATCGATGGA GACATCACCCTGGGAGGCCTGTTTCCCGTCCACGGTCGCGGCTCCGAGGGCAAGGCCTGCGGCGAGTTGA AGAAGGAGAAAGGTATCCACCGGCTGGAGGCCATGCTCTTTGCCCTGGACCGCATCAACAACGACCCGGA CCTACTGCCCAACATCACGTTGGGCGCCCGCATTCTGGACACCTGCTCAAGGGATACCCACGCCCTGGAG CAGTCCCTGACCTTTGTGCAGGCGCTCATCGAGAAGGACGGCACGGAGGTCCGCTGCGGCAGCGGGGGCC CACCCATCATCACCAAGCCTGAACGAGTGGTGGGTGTCATCGGAGCTTCGGGGAGCTCCGTCTCGATCAT GGTGGCCAACATCCTCCGCCTCTTCAAGATCCCCCAGATCAGCTACGCCTCCACGGCTCCCGACTTGAGT GATAACAGCCGCTATGACTTCTTCTCCCGGGTCGTGCCCTCGGACACATACCAGGCCCAGGCCATGGTGG ACATCGTCCGGGCCCTCAAGTGGAACTATGTGTCCACGCTGGCCTCAGAGGGTAGCTACGGCGAGAGCGG CGTGGAGGCCTTTATCCAGAAGTCCCGAGAGAACGGAGGCGTGTGCATTGCCCAGTCGGTGAAGATTCCA CGGGAACCCAAGACCGGGGAGTTTGACAAGATCATCAAACGCCTTCTGGAAACGTCCAATGCCAGAGCCA TCATCATCTTTGCCAACGAGGATGATATCAGGAGGGTGCTGGAGGCAGCGCGCAGGGCCAACCAGACCGG CCACTTCTTTTGGATGGGTTCTGATAGCTGGGGCTCCAAGAGCGCCCCCGTGCTGCGCCTTGAGGAAGTG GCTGAAGGTGCAGTCACCATTCTTCCCAAGAGGACGTCTGTGCGAGGGTTTGACCGATACTTCTCCAGCC GCACGCTTGACAACAACAGGCGCAACATCTGGTTTGCTGAGTTCTGGGAGGACAACTTCCATTGCAAGTT GAGCCGCCACGCGCTCAAGAAGGGAAGCCACATCAAGAAGTGCACCAACCGAGAGCGCATCGGGCAGGAC TCGGCCTACGAACAGGAGGGGAAGGTGCAGTTTGTGATCGACGCCGTGTACGCCATGGGCCATGCTCTGC ACGCCATGCATCGTGACCTGTGTCCCGGCCGCGTAGGACTCTGCCCTCGAATGGACCCTGTGGATGGCAC CCAGCTGCTTAAGTACATCAGAAACGTCAACTTCTCAGGCATCGCCGGGAACCCGGTGACCTTCAACGAG AACGGAGACGCGCCAGGGCGTTATGACATCTACCAGTACCAACGTCGCAACGGCTCGGCTGAGTACAAGG TCATCGGCTCATGGACAGACCACTTGCACCTCAGAATAGAGCGGATGCAGTGGCCAGGGAGTGGCCAGCA GCTGCCACGCTCCATCTGCAGCCTGCCCTGCCAGCCAGGCGAGCGGAAGAAGACGGTGAAGGGCATGGCT TGCTGCTGGCACTGCGAGCCCTGCACGGGGTACCAGTACCAGGTGGACCGCTACACCTGTAAGACCTGCC CCTATGACATGCGGCCCACGGAGAACCGCACGAGCTGCCAGCCCATACCCATTGTCAAGTTGGAGTGGGA CTCACCCTGGGCTGTGCTGCCCCTCTTCCTGGCTGTGGTGGGCATTGCTGCCACGCTGTTCGTGGTGGTC ACTTTTGTGCGCTACAACGACACTCCGATCGTCAAGGCCTCGGGCCGGGAGCTGAGCTACGTCCTGCTGG CGGGCATCTTTCTCTGCTATGCCACCACCTTCCTCATGATCGCAGAGCCTGACCTGGGGACCTGTTCACT CCGCCGCATCTTCCTGGGGCTTGGCATGAGCATCAGCTACGCGGCCCTGCTGACCAAGACCAACCGCATC TACCGCATCTTTGAGCAGGGCAAGCGGTCGGTCAGCGCCCCACGGTTCATCAGCCCCGCCTCGCAGCTGG CCATCACCTTCGTCCTCATCTCGCTGCAGTTGCTTGGCATCTGCGTGTGGTTCGTGGTGGACCCCTCCCA CTCGGTGGTGGACTTCCAGGACCAGCGGACACTTGACCCCCGCTTCGCCCGGGGTGTGCTCAAGTGTGAC ATCTCGGACCTGTCGCTCATCTGCCTCCTGGGCTACAGCATGCTGCTGATGGTCACGTGTACTGTGTATG CCATCAAGACTCGAGGCGTGCCTGAGACCTTCAATGAGGCCAAGCCCATCGGCTTCACCATGTACACCAC CTGCATCGTCTGGCTGGCCTTCATCCCCATCTTTTTTGGCACCTCGCAGTCGGCTGACAAGGTAACCTCT GAGGCCCTGCCCGTGGAATTCAGCCCGCCATTGCTGGCACATAATTAAAGCATGTCACCATAATTTGCCT TTTTTCCCCCTTACAAAGCCATAATTATGTTTTTATTGCAAGTGAAGGGGGGAAAGCAGGGGAAAAGAAA ACAAAGAGAGGCGGGGCCGGGGCAGGAACCCGGGCTAGGCTTCCTTAGCCAGGGTCTCCCTGCCTTCTGG GGGCCACTTCCCCTCAGCATCTCTGGGAACTCCCTCTAGCCCTGCCTGATGGCCCGCCCAGGGGAGAGCA GAAACTGTGTCCGAAGGGATGATTCCTAAGTTGGGAGCAGGCACGAGGGAGCCCCTGCATCTTCTGCAGT TGTTGGAAATTTTCCATAATAAAAAGTTAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6402528 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6402528 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7625 same embryo
	EMAGE:7630 same embryo
	EMAGE:7627 same embryo
	EMAGE:7626 same embryo
	EMAGE:7628 same embryo
	EurExpress:euxassay_004721 same experiment
	MGI:4825240 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source