EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7892

Casz1 castor homolog 1, zinc finger (Drosophila) ( MGI:1196251)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012815. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012815_01	euxassay_012815_02	euxassay_012815_03	euxassay_012815_04

euxassay_012815_05	euxassay_012815_06	euxassay_012815_07	euxassay_012815_08	euxassay_012815_09

euxassay_012815_10	euxassay_012815_11	euxassay_012815_12	euxassay_012815_13	euxassay_012815_14

euxassay_012815_15	euxassay_012815_16	euxassay_012815_17	euxassay_012815_18	euxassay_012815_19

euxassay_012815_20	euxassay_012815_21	euxassay_012815_22	euxassay_012815_23	euxassay_012815_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7892_wholemount_strong.wlz

7892_wholemount_moderate.wlz

7892_wholemount_weak.wlz

7892_wholemount_possible.wlz

7892_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7892_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 07 08 19 20 weak expression: see section 06

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 15 16 weak expression: see section 08

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 19 21 weak expression: see section 05

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 18 weak expression: see section 06

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 09 17 18 19 20 21 weak expression: see section 05 06 07 08

vagus x ganglion

weak

regional

weak expression: see section 07 17

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 18

trigeminal v nerve

moderate

regional

moderate expression: see section 09 17

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 14 15 weak expression: see section 13 16

lower jaw incisor

weak

regional

weak expression: see section 15 16

lower jaw molar

weak

regional

weak expression: see section 07 08 18

upper jaw incisor

weak

regional

weak expression: see section 15 16

upper jaw molar

weak

regional

weak expression: see section 07 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38200
Entity Detected:	Casz1, castor homolog 1, zinc finger (Drosophila) ( MGI:1196251)
Sequence:	sense strand is shown >T38200 CGAGGAATACATCCGTAAGCTCAAGGCCGGCGAGCAACTTCCCTGGCCAGCCCACGGGAGCAAAGCCGAG GACCGGGCAGGCAAGGAGGTGGTGGGTCCCTTACCCAGCCTACGGCTGCCCAGCAACACGGCCCACCTGG AAACCAAGGCCACCATCCTGCCACTGCCATCACACAGCAGTGTCCAGATGCAGAATCTGGTAGCTCGTGC TTCCAAGTATGACTTCTTCATCCACAAACTGAAGACAGGCGAGAACCTGAGGCCCCAGAATGGAAGCACT TACAAGAAGCCATCCAAGTATGACCTGGAGAATGTCAAGTACTTGCACCTCTTCAAACCCGGGGAAGGCA GCCCTGACATGGGCGGGGCCATCGCCTTCAAGACAGGCAAGGTGGGGCGCCCCTCTAAGTACGACGTTCG GGGCATCCAGAAGCCAGGCCCTACCAAGATTCCGCCCGCCCCCAGCCTGGTTCCTACACCCCTCACCAAT GTGCCCAGTGCTCCCAGCACCCCCGGACCAGGACCGGAGCCACCTGCCTCCTTGTCCTTCAACACTCCCG AGTACCTGAAGTCAACCTTTTCCAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 103682. Forward Primer - name:103682_F_cDNA_D4Ertd432e, sequence:CGAGGAATACATCCGTAAGCTC; Reverse Primer - name:103682_N_SP6_cDNA_D4Ertd432e, sequence:TTGGAAAAGGTTGACTTCAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7888 same embryo
	EMAGE:7890 same embryo
	EMAGE:7891 same embryo
	EMAGE:7889 same embryo
	EurExpress:euxassay_012815 same experiment
	MGI:4823648 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source